The efficiency nof fluorescence resonance energy transfer (FRET) is given by: R$ = R + R° A solution is prepared containing linear molecules, each consisting of an anthracene donor and a tryptophyl peptide acceptor separated by n L-prolyl linker units. The solution absorbs 0.230 mJ of laser radiation with a wavelength of 273 nm and emits 1.35 ×10² mJ of fluorescence at a wavelength of 293 nm. Given that R, = 2.20 nm, ø; = 0.36 for anthracene, and the approximate length of one linker %3D unit is 0.34 nm, determine n.

The efficiency nof fluorescence resonance energy transfer (FRET) is given by: R$ = R + R° A solution is prepared containing linear molecules, each consisting of an anthracene donor and a tryptophyl peptide acceptor separated by n L-prolyl linker units. The solution absorbs 0.230 mJ of laser radiation with a wavelength of 273 nm and emits 1.35 ×10² mJ of fluorescence at a wavelength of 293 nm. Given that R, = 2.20 nm, ø; = 0.36 for anthracene, and the approximate length of one linker %3D unit is 0.34 nm, determine n.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter30: Capillary Electrophoresis, Electrochromatography, And Field-flow Fractionation

Section: Chapter Questions

Problem 30.9QAP

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