Table 17G.3 Values of R, for some donor-acceptor pairs* Donor Acсеptor R/nm Naphthalene Dansyl 2.2 Dansyl ODR 4.3 Pyrene Coumarin 3.9 1.5-I AEDANS FITC 4.9 Tryptophan 1.5-I AEDANS 2.2 Tryptophan Haem (heme) 2.9 "Additional values may be found in J.R. Lacowicz, Principles of fluorescence spectros- copy, Kluwer Academic/Plenum, New York (1999). *Abbreviations: Dansyl: 5-dimethylamino-1-naphthalenesulfonic acid FITC: fluorescein 5-isothiocyanate 1.5-I AEDANS: 5-(((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (3) ODR: octadecyl-rhodamine
Table 17G.3 Values of R, for some donor-acceptor pairs* Donor Acсеptor R/nm Naphthalene Dansyl 2.2 Dansyl ODR 4.3 Pyrene Coumarin 3.9 1.5-I AEDANS FITC 4.9 Tryptophan 1.5-I AEDANS 2.2 Tryptophan Haem (heme) 2.9 "Additional values may be found in J.R. Lacowicz, Principles of fluorescence spectros- copy, Kluwer Academic/Plenum, New York (1999). *Abbreviations: Dansyl: 5-dimethylamino-1-naphthalenesulfonic acid FITC: fluorescein 5-isothiocyanate 1.5-I AEDANS: 5-(((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (3) ODR: octadecyl-rhodamine
Chapter10: Effect Of Electrolytes On Chemical Equilibria
Section: Chapter Questions
Problem 10.11QAP
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An amino acid on the surface of a protein was labelled covalently with 1.5-I AEDANS and another was labelled covalently with FITC. The fluorescence quantum yield of 1.5-I AEDANS decreased by 10 per cent due to quenching by FITC. What is the distance between the amino acids? (Refer to Table 17G.3 for the appropriate value of R0.)
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