Table 17G.3 Values of R, for some donor-acceptor pairs* Donor Acсеptor R/nm Naphthalene Dansyl 2.2 Dansyl ODR 4.3 Pyrene Coumarin 3.9 1.5-I AEDANS FITC 4.9 Tryptophan 1.5-I AEDANS 2.2 Tryptophan Haem (heme) 2.9 "Additional values may be found in J.R. Lacowicz, Principles of fluorescence spectros- copy, Kluwer Academic/Plenum, New York (1999). *Abbreviations: Dansyl: 5-dimethylamino-1-naphthalenesulfonic acid FITC: fluorescein 5-isothiocyanate 1.5-I AEDANS: 5-(((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (3) ODR: octadecyl-rhodamine

Fundamentals Of Analytical Chemistry
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Chapter10: Effect Of Electrolytes On Chemical Equilibria
Section: Chapter Questions
Problem 10.11QAP
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An amino acid on the surface of a protein was labelled covalently with 1.5-I AEDANS and another was labelled covalently with FITC. The fluorescence quantum yield of 1.5-I AEDANS decreased by 10 per cent due to quenching by FITC. What is the distance between the amino acids? (Refer to Table 17G.3 for the appropriate value of R0.)

Table 17G.3 Values of R, for some donor-acceptor pairs*
Donor
Acсеptor
R/nm
Naphthalene
Dansyl
2.2
Dansyl
ODR
4.3
Pyrene
Coumarin
3.9
1.5-I AEDANS
FITC
4.9
Tryptophan
1.5-I AEDANS
2.2
Tryptophan
Haem (heme)
2.9
"Additional values may be found in J.R. Lacowicz, Principles of fluorescence spectros-
copy, Kluwer Academic/Plenum, New York (1999).
*Abbreviations:
Dansyl: 5-dimethylamino-1-naphthalenesulfonic acid
FITC: fluorescein 5-isothiocyanate
1.5-I AEDANS: 5-(((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (3)
ODR: octadecyl-rhodamine
Transcribed Image Text:Table 17G.3 Values of R, for some donor-acceptor pairs* Donor Acсеptor R/nm Naphthalene Dansyl 2.2 Dansyl ODR 4.3 Pyrene Coumarin 3.9 1.5-I AEDANS FITC 4.9 Tryptophan 1.5-I AEDANS 2.2 Tryptophan Haem (heme) 2.9 "Additional values may be found in J.R. Lacowicz, Principles of fluorescence spectros- copy, Kluwer Academic/Plenum, New York (1999). *Abbreviations: Dansyl: 5-dimethylamino-1-naphthalenesulfonic acid FITC: fluorescein 5-isothiocyanate 1.5-I AEDANS: 5-(((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (3) ODR: octadecyl-rhodamine
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