The hydrolysis of pyrophosphate to orthophosphate drives biosynthetic reactions such as DNA synthesis. In Escherichia coli, a pyrophosphatase catalyzes this hydrolytic reaction. The pyrophosphatase has a mass of 120 kDa and consists of six identical subunits. A unit of activity for this enzyme, U, is the amount of enzyme that hydrolyzes 10 umol of pyrophosphate in 15 minutes. The purified enzyme has a Vmax of 2800 U per milligram of enzyme. When (S) >> KM, how many micromoles of substrate can 1 mg of enzyme hydrolyze per second? umol-s-, mg-! max If cach enzyme subunit has one active site, how many micromoles of active sites, or (E]r, are there in 1 mg of enzyme? (E= umol - mg-

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A: Vmax. = 2800 U/ mg of enzyme 1 U = 10 μmol/15 min. 1 min. = 60 second 15 min =             =60 sec.…

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