The majority of bacterial mutations that need isoleucine also require valine for growth. Why? Which enzyme or process would be deficient in a mutant that requires solely isoleucine for growth (rather than valine)?
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The majority of bacterial mutations that need isoleucine also require valine for growth. Why? Which enzyme or process would be deficient in a mutant that requires solely isoleucine for growth (rather than valine)?
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- Most bacterial mutants that require isoleucine for growth also require valine.Why? Which enzyme or reaction would be defective in a mutant requiringonly isoleucine (not valine) for growth?Why is there no net gain of methionine if homocysteine isconverted to methionine with S-adenosylmethionine as the methyldonor?Why would the mutation Ile -> Asp be likely to destabilize a protein? Why would the mutation Ile ->Val be likely to destabilize a protein? Why would the mutation Val -> Ile be likely to destabilize a protein?
- In studies using repeating copolymers, AC . . . incorporates threonine and histidine, and CAACAA . . . incorporates glutamine, asparagine, and threonine. What triplet code can definitely be assigned to threonine?In pyrimidine nucleosides, the anti conformation predominates. Explain. Do the purine nucleosides have similar interactions?If this molecule can inhibit toxic effects at 0.5mM against gastric carcinoma cells, does it mean that the dimer of this nucleoside analogue will exhibit an IC50 of about 0.25mM?
- Describe the chemical difference between mannose and heptyl alpha-D-mannose. Why does FimH have a higher affinity for heptyl a-D-mannose versus methyl a-D-mannose?What advantages are there for synthesizing an inactive protein that must subsequently be activated by posttranslationalmodifications?In some proteins, the side chain of serine appears to undergo ionization. Explain why ionization would be facilitated by the presence of an aspartate residue nearby.
- In a line of human cells grown in culture, a geneticist isolates a temperature-sensitive mutation at a locus that encodes an acetyltransferase enzyme; at temperatures above 38°C, the mutant cells produce a nonfunctional form of the enzyme. What would be the most likely effect of this mutation if the cells were grown at 40°C?What is the name for Gly-Tyr-Leu-Val Sequence?How does the action and mutagenic effect of 5-Bromouracil differ from that of nitrous acid? explain.