The mobility of an ion depends on its charge and if a large molecule, such as a protein, can be contrived to have zero net charge, then it does not respond to an electric field. This 'isoelectric point' can be reached by varying the pH of the medium. The speed with which bovine serum albumin (BSA) moves through water under the influence of an electricfield was monitored at several values of pH, and the data are listed below.pH 4.20 4.56 5.20 5.65 6.30 700Speed/(μm s-1) 0.40 0.18 -0.25 -0.51 -0.90 -125By drawing a plot of speed against pH to find the pH at which the speed is zero, determine the isoelectric point of the protein.
The mobility of an ion depends on its charge and if a large molecule, such as a protein, can be contrived to have zero net charge, then it does not respond to an electric field. This 'isoelectric point' can be reached by varying the pH of the medium. The speed with which bovine serum albumin (BSA) moves through water under the influence of an electricfield was monitored at several values of pH, and the data are listed below.pH 4.20 4.56 5.20 5.65 6.30 700Speed/(μm s-1) 0.40 0.18 -0.25 -0.51 -0.90 -125By drawing a plot of speed against pH to find the pH at which the speed is zero, determine the isoelectric point of the protein.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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The mobility of an ion depends on its charge and if a large molecule, such as a protein, can be contrived to have zero net charge, then it does not respond to an electric field. This 'isoelectric point' can be reached by varying the pH of the medium. The speed with which bovine serum albumin (BSA) moves through water under the influence of an electric
field was monitored at several values of pH, and the data are listed below.
pH 4.20 4.56 5.20 5.65 6.30 700
Speed/(μm s-1) 0.40 0.18 -0.25 -0.51 -0.90 -125
By drawing a plot of speed against pH to find the pH at which the speed is zero, determine the isoelectric point of the protein.
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