The net charge on this peptide at pH 9.5 is

Human Heredity: Principles and Issues (MindTap Course List)
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Chapter9: Gene Expression And Gene Regulation
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Problem 19QP: Below is the structure of glycine. Draw a tripeptide composed exclusively of glycine. Label the...
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Can you please help find what the net charge of ph 9.5 is?
Peptide lonization
Consider a peptide with the sequence Ser-His-Glu-Cys-Arg-Gln.
You will be asked to state the charge on the functional groups of this
peptide at pH 1 and pH 9.5.
You will also be asked to calculate the net charge on the peptide. You may
find it convenient to write down the charge on each group on a piece of
paper as you go along, so that you can add them up at the end.
Assume the following generic pK, values for ionizable functional groups:
a-carboxyl:
side-chain carboxyl:
imidazole:
3.1
4.1
6.0
a-amino:
8.0
thiol:
8.3
E-amino:
10.8
aromatic hydroxyl:
guanidino:
10.9
12.5
Transcribed Image Text:Peptide lonization Consider a peptide with the sequence Ser-His-Glu-Cys-Arg-Gln. You will be asked to state the charge on the functional groups of this peptide at pH 1 and pH 9.5. You will also be asked to calculate the net charge on the peptide. You may find it convenient to write down the charge on each group on a piece of paper as you go along, so that you can add them up at the end. Assume the following generic pK, values for ionizable functional groups: a-carboxyl: side-chain carboxyl: imidazole: 3.1 4.1 6.0 a-amino: 8.0 thiol: 8.3 E-amino: 10.8 aromatic hydroxyl: guanidino: 10.9 12.5
The net charge on this peptide at pH 9.5 is
-3
-1
+2
+1
-2
+3
Transcribed Image Text:The net charge on this peptide at pH 9.5 is -3 -1 +2 +1 -2 +3
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