The significance of using immersion oil when using 1OOX objective lens isThe significance of using immersion oll when uskng 100X objective lens is:It will increase the contrastby having same refractive index as the lens, loss of light by refraction, reflectionand diffraction is minimized.the specimens need not be killed, fixed and stainedmore light is captured for better illumination, allowing the specimen to fluorescei.il.iii.iv.A light microscope should have the following featurescontrastResolutionmagnificationfluorescenceii.iv.

Answer. Microscopy is a technique for making very small things visible to the unaided eye. An…

Answered: The significance of using immersion oll…

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter16: Fiber Optics And Lasers In Bioinstrumentation

Section: Chapter Questions

Problem 6P

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As with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images. a. refractive index b. light absorption c. natural fluorescence d. walls and coverings e. natural pigmentation
It is desirable that the microscope objectives be parfocal because the specimen will be .................. a)blurry due to the changes in the refractive index of the medium b)stay in focus when the light is turned off c)have more contrast and enhanced details d)remain in focus if the magnification is changed e)none of the answer choices
The total magnification of a microscope is calculated by:(a) Addition of the objective lens and ocular lens magnifi-cation powers(b) Multiplication of the objective lens and ocular lens mag-nification powers(c) Multiplication of the objective lens and condenser lensmagnification powers(d) The objective lens power squared(e) None of the above
In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.
The FOV on low power of a microscope is measured to be 4.5mm. Using the chart below and the above listed formulas calculate: FOvx = FOvlow X Maglow Magx S.S. = FOvx # times the specimen fits across the FOv Total Mag = Ocular lens x objective lens a) What is the FOV on High Power? (Magnification on High power is 40x) Can you please include the units too. asap please.
Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…
How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensity
The diffraction limit is an unbeatable barrier limiting the effective resolution of light microscopy due to the inherent wavelength of light.True or False
Which of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the center
Microscopic objects often are measured in micrometers. A micrometer equals1/1000 of a millimeter and is symbolized by μm. What is the diameter of thescanning power field of view in micrometers? ___________
Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the total magnification of the image under microscope? Show your solution.
Adjusting the objective aperture is an important step to obtain phase contrast in TEM. Compare the differences in objective aperture operation between diffraction contrast and phase contrast.

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