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- Microscopic objects often are measured in micrometers. A micrometer equals1/1000 of a millimeter and is symbolized by μm. What is the diameter of thescanning power field of view in micrometers? ___________1. What total magnification will be achieved if the 10× eyepiece and the 10× objective are used?___________Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's Law
- 2. Matching the type of instrument you would use to measure the following Group of answer choices Volume [ Choose ] meter stick graduated cylinder test tube scale Distance [ Choose ] meter stick graduated cylinder test tube scale Weight [ Choose ] meter stick graduated cylinder test tube scale3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A.The cell is being viewed under high power. Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm
- Match the following items: [4] 1. 540 nm A. Wavescan 2. Quantity of light of a specific wavelength absorbed by a sample B. Standard series 3. Reference samples with a concentration range C. Absorbance 4. Absorbance values at all wavelengths D. Purpleif the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?Which of the following results in green output in spectrophotometry experiment? a. Violet light b. Blue light c. Green light d. Yellow light
- f I use the 10x eyepiece and the 40x objective, what is the magnification? Select one: 4 diameters 40 diameters 4000 diameters 400 diameters1. after going through all 4 objectives, which would give you the largest field of view? greatest depth of focus? why? 2. how do you calculate the total magnification of a specimen? show sample3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm
