The table contains data about how different ASFV mutants affect enzyme activity-- for example the D29A mutant, where the D at position 29 was mutated to an A. Select three mutants at random, and for each mutant explain how that mutation could affect intermolecular force interactions (an example: hydrogen bonding could be disrupted by this mutation because… Kcat/Km(M¯1 s-1)EnzymeКт (иM)Keat (s)aDUTWTADUTD29AaDUTR71AaDUT$72AaDUT$73AADUTL89AaDUT190AaDUTD91AaDUTY94AaDUTQ120AADUTA147aDUTR149AADUTR149KaDUTF154YSDUTWT0.98 ± 0.24.37 + 0.154.46 × 1064.62 × 10*6.89 ± 0.20.31 ± 0.14.89 ± 0.354.16 ± 0.190.48 + 0.29.85 x 10*1.34 x 1060.54 × 101.58 x 1065.58 + 0.35.44 ± 0.52.94 ± 0.70.47 + 0.260.75 + 0.216.76 ± 1.210.58 +0.43.51 x 10%4.51 + 0.70.15 + 0.126.45 ± 0.20.58 ± 0.131.5 + 0.15.58 ± 0.60.33 x 1050.56 x 1059.60 × 106

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Description: The table contains data about how different ASFV mutants affect enzyme activity-- for example the D29A mutant, where the D at position 29 was mutated to an A. Select three mutants at random, and for each mutant explain how that mutation could affect

Mutations are the abrupt changes in DNA molecule. DNA is made up of nucleotides and nucleotides…

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Biology 2e

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Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter33: The Animal Body: Basic Form And Function

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The table contains data about how different ASFV mutants affect enzyme activity-- for example the D29A mutant, where the D at position 29 was mutated to an A. Select three mutants at random, and for each mutant explain how that mutation could affect intermolecular force interactions (an example: hydrogen bonding could be disrupted by this mutation because…

Kcat/Km
(M¯1 s-1)
Enzyme
Кт (иM)
Keat (s)
aDUTWT
ADUTD29A
aDUTR71A
aDUT$72A
aDUT$73A
ADUTL89A
aDUT190A
aDUTD91A
aDUTY94A
aDUTQ120A
ADUTA147
aDUTR149A
ADUTR149K
aDUTF154Y
SDUTWT
0.98 ± 0.2
4.37 + 0.15
4.46 × 106
4.62 × 10*
6.89 ± 0.2
0.31 ± 0.1
4.89 ± 0.35
4.16 ± 0.19
0.48 + 0.2
9.85 x 10*
1.34 x 106
0.54 × 10
1.58 x 106
5.58 + 0.3
5.44 ± 0.5
2.94 ± 0.7
0.47 + 0.26
0.75 + 0.2
16.76 ± 1.21
0.58 +0.4
3.51 x 10%
4.51 + 0.7
0.15 + 0.1
26.45 ± 0.2
0.58 ± 0.13
1.5 + 0.1
5.58 ± 0.6
0.33 x 105
0.56 x 105
9.60 × 106

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