The thiolate anion of CoA acts as a/an nucleophile attacking the carbonyl carbon of the enzyme bound intermediate, displacing AMP and forming a fatty acyl CoA.
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- Using the ActiveModel for enoyl-CoA dehydratase, give an example of a case in which conserved residues in slightly different positions can change the catalytic rate of reaction.Figure 27.3 illustrates the response of R (ATP-regenerating) and U (ATP-utilizing) enzymes to energy charge. a. Would hexokinase be an R enzyme or a U enzyme? Would glutamine: PRPP amidotransferase, the second enzyme in purine biosynthesis, be an R enzyme or a U enzyme? b. If energy charge = 0.5: Is the activity of hexokinase high or low? Is ribose-5-P pyrophosphokinase activity high or low? c. If energy charge = 0.95: Is the activity of hexokinase high or low? Is ribose-5-P pyrophosphokinase activity high or low?Distinguishing the Mechanisms of Class I and Class I Aldolases Fructose bisphosphate aldolase in animal muscle is a class 1 aldolase, which forms a Schiff base intermediate between substrate (for example. fructose-1, 6-bisphosphate or dihydroxyacetone phosphate) and a lysine at the active site (see Figure I8.12). The chemical evidence for this intermediate conies from studies with aldolase and the reducing agent sodium borohydride, NaBH4. Incubation of the enzyme with dihydroxyacetone phosphate and NaBH4 inactivates the enzyme. Interestingly, no inactivation is observed if NabH4 is added to the enzyme in the absence of substrate. Write a mechanism that explains these observations and provides evidence for the formation of a Schiff base intermediate in the aldolase reaction.
- Using the ActiveModel for aldose reductase, describe the structure of the TIM barrel motif and the structure and location of the active site.A Deficiency on 3-Phosphogtycerate Dehydrogenase Can Affect Amino Acid Metabolism Although serine is a nonessential amino acid, serine deficiency syndrome has been observed in humans. One such form of the syndrome is traceable to a deficiency in 3-phosphoglycerate dehydrogenase (see Figure 25.31). Individuals with this syndrome not only arc serine-deficient but also arc impaired in their ability to synthesize another common ammo acid, as well as a class of lipids. Describe why.The Reactions and Meehanisms of the Leloir Pathway Write the reactions that permit galactose to be utilized in glycolysis. Write a suitable mechanism, tor one of these reactions.
- Which of the following biochemical conversions can be carried out by the least number of proteins? Assume proper metabolic conditions, abundance of cofactors, necessary accessory molecules, etc. 1 propionyl-CoA → 1 succinyl-CoA 1 palmitate (16:0) → 8 acetyl-CoA 1 acetyl-CoA + 7 malonyl-CoA → 1 palmitate (16:0) 1 palmitate (16:0) → 1 acetyl-CoA + 1 myristic acid (14:0) 1 acetyl-CoA → 1 succinate Please answer very soon will give rating surelyIn the context of fatty acid oxidation to acetyl CoA, indicate whether each of the following substances is involved in (1) fatty acid activation, (2) fatty acid trans-port, or (3) b-oxidation pathway. More than one choice may be correct in a given situation. a. AMp b. FAD c. Acyl CoA d. H2OAminolevulinate (ALA) synthase is a pyridoxal phosphate containing enzyme displaying a non-sequential (ping-pong) enzyme mechanism. It catalyses the reaction: glycine + succinyl-CoA ⇌ aminolevulinate + CO2 Draw a Cleland diagram (diagram illustrating when the substrates and products form an enzyme substrate complex) for this reaction if glycine is the first substrate to bind.
- Many components of the glycolytic pathway and the citricacid cycle are direct exit or entry points to metabolic pathways ofother substances. Indicate another pathway available to the following compounds:(a) Fructose-6-phosphate(b) Oxaloacetate(c) Glucose-6-phosphate(d) Acetyl-CoA(e) Glyceraldehyde-3-phosphate(f) -Ketoglutarate(g) Dihydroxyacetone phosphate(h) Succinyl-CoA(i) 3-Phosphoglycerate(j) Fumarate(k) Phosphoenolpyruvate(l) Citrate(m) PyruvateThe archaebacterium Haloarcula marismortui can use acetate (which is converted to acetyl-CoA) as a precursor for carbohydrate synthesis, but it lacks isocitrate lyase and therefore cannot use the glyoxylate pathway. Instead, it converts isocitrate to α-ketoglutarate, which is then converted to the amino acid methylaspartate in two more steps. This five-carbon compound is modifi ed and broken down to release glyoxylate. Describe the three reactions that lead from isocitrate to methylaspartate.Which of the following is most helpful in ensuring that wasteful (unproductive) hydrolysis of acetyl CoA does not occur in the reaction catalyzed by citrate synthase? Group of answer choices Citrate synthase is a tetramer Induced fit ensures oxaloacetate binds before acetyl CoA 3 molecules of oxaloacetate bind for every 1 molecule of acetyl CoA Citrate inhibits the reaction Which of the following catalyzes a step that does NOT produce CO2? Group of answer choices alpha-ketoglutarate dehydrogenase complex malate dehydrogenase pyruvate dehydrogenase complex isocitrate dehydrogenase Citrate synthase is regulated by... Group of answer choices Concentrations of substrate and products Fructose-2,6-Bisphosphate Covalent modification Allosteric regulation by the levels of NADDH