There are many ways you can accomplish a 1000-fold dilution. Propose 3 different serial dilution methods that will accomplish a dilution with a 1:1000 dilution factor. 2. Suppose you count 35 bacterial cells from a solution that has gone through the following serial dilutions: two 10-fold dilutions, followed by a 5-fold dilution, followed by 2-fold dilution. What should be the bacterial count from the original solution? Show your work! 3. When making a dilution you need to put different volumes of the stock solution and the buffer. Suppose you are doing a 2-fold dilution, where both volumes are the same. Will you use the same pipette tip to extract the stock and the buffer solution? Explain your reasoning.

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
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1. There are many ways you can accomplish a 1000-fold dilution. Propose 3 different serial dilution methods that will accomplish a dilution with a 1:1000 dilution factor.

2. Suppose you count 35 bacterial cells from a solution that has gone through the following serial dilutions: two 10-fold dilutions, followed by a 5-fold dilution, followed by 2-fold dilution. What should be the bacterial count from the original solution? Show your work!

3. When making a dilution you need to put different volumes of the stock solution and the buffer. Suppose you are doing a 2-fold dilution, where both volumes are the same. Will you use the same pipette tip to extract the stock and the buffer solution? Explain your reasoning.

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