1) based on the difference in appearance between mixed culture streak plates and broth culture plates, what would lead you to believe that you have created a pure culture when you inoculated the broths? 2) when using the loop dilution technique to produce pour plates, did all the plates produce isolated colonies? If your goal was to ultimately create pure cultures of E. coli, M. luteus, and S. morcescens, which plate or which dilution would you use, and why would you use this plate? 3) What are the advantages and disadvantages of streak plating compared with pour plating?

1) based on the difference in appearance between mixed culture streak plates and broth culture plates, what would lead you to believe that you have created a pure culture when you inoculated the broths? 2) when using the loop dilution technique to produce pour plates, did all the plates produce isolated colonies? If your goal was to ultimately create pure cultures of E. coli, M. luteus, and S. morcescens, which plate or which dilution would you use, and why would you use this plate? 3) What are the advantages and disadvantages of streak plating compared with pour plating?

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter13: Aquatic Structures And Equipment

Section: Chapter Questions

Problem 7E

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Similar questions

What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?
After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.
you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each other from this mixed culture?( Besides using plate technique)
A culture of E. coli is diluted as follows:(1) 65mL are added to 435mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-3 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?c) How many milliliters of water is needed to make a 10-3 dilution using 1000 uL from the original culture?
If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many fewer bacteria the broth used for the plate picture here), would you expect to see single, isolated colored in quadrant 4 or quadrant3? Explain your answer.
Are the large numbers of microorganisms found in the mouth cause for concern? Explain. How can you determine whether a culture or subculture is pure? What kinds of clinical speciments may yield a mixed flora in bacterial cultures? Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
Why do you think the continuous culture has higher chances of contamination than batch and fed-batch culture?
What is the difference between a mixed culture and a contaminated culture if they both contain more than one type of organism ?
The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?
In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL
You were given a mixed nutrient agar broth culture of bacteria 1a. How will you determine the different types of bacteria present in the mixed culture without using an agar plate 1b. How will you make a pure culture of these bacteria on a slant nutrient agar 1c. How will you identify these bacteria from the pure culture without using an agar plate
What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 × 106 cells/mL?