(This is taken mainly from Lehninger Principles of Biochemistry, 6th ed., ch. 6, #12, p. 239.) The kinetic data below are for the cyclooxygenase reaction catalyzed by prostaglandin H2 synthase ("COX"), producing prostaglandin G2, the precursor of other prostaglandins and of thromboxanes. Velocity was measured as a function of the concentration of the substrate arachidonic acid in the absence and in the presence of 10 mg/ml ibuprofen (C13H1802). The molecular mass of the undissociated acid form of ibuprofen is 206 g/mol. (Structure of the anionic form is on p. 846 of Lehninger Principles of Biochemistry, 6th ed.) The reciprocals of velocity and of [substrate] have been calculated for you! [arachidonic acid] (mm) 0.5 1.0 1.5 2.5 3.5 1/[S] (mM)-1 2.00 1.00 0.667 0.400 0.286 I velocity (rate of formation of PGG2) (mm/min) 23.5 32.2 36.9 41.8 44.0 1/v (mm/min)-1 0.0426 0.0311 0.0271 0.0239 0.0227 Velocity with 10 mg/ml ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 1/v with ibuprofen (mm/min-1 0.0600 0.0396 0.0328 0.0270 0.0257 Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine Km and Vmax in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units for all numerical answers.
(This is taken mainly from Lehninger Principles of Biochemistry, 6th ed., ch. 6, #12, p. 239.) The kinetic data below are for the cyclooxygenase reaction catalyzed by prostaglandin H2 synthase ("COX"), producing prostaglandin G2, the precursor of other prostaglandins and of thromboxanes. Velocity was measured as a function of the concentration of the substrate arachidonic acid in the absence and in the presence of 10 mg/ml ibuprofen (C13H1802). The molecular mass of the undissociated acid form of ibuprofen is 206 g/mol. (Structure of the anionic form is on p. 846 of Lehninger Principles of Biochemistry, 6th ed.) The reciprocals of velocity and of [substrate] have been calculated for you! [arachidonic acid] (mm) 0.5 1.0 1.5 2.5 3.5 1/[S] (mM)-1 2.00 1.00 0.667 0.400 0.286 I velocity (rate of formation of PGG2) (mm/min) 23.5 32.2 36.9 41.8 44.0 1/v (mm/min)-1 0.0426 0.0311 0.0271 0.0239 0.0227 Velocity with 10 mg/ml ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 1/v with ibuprofen (mm/min-1 0.0600 0.0396 0.0328 0.0270 0.0257 Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine Km and Vmax in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units for all numerical answers.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![(This is taken mainly from Lehninger Principles of Biochemistry, 6th ed., ch. 6, #12, p. 239.)
The kinetic data below are for the cyclooxygenase reaction catalyzed by prostaglandin H2 synthase
("COX"), producing prostaglandin G2, the precursor of other prostaglandins and of thromboxanes.
Velocity was measured as a function of the concentration of the substrate arachidonic acid in the
absence and in the presence of 10 mg/ml ibuprofen (C13H1802). The molecular mass of the
undissociated acid form of ibuprofen is 206 g/mol. (Structure of the anionic form is on p. 846 of
Lehninger Principles of Biochemistry, 6th ed.) The reciprocals of velocity and of [substrate] have
been calculated for you!
IOLC
[arachidonic acid]
(mM)
1/[S]
(mM)"
velocity (rate
of formation
1/v
Velocity with
10 mg/ml
ibuprofen
(mM/min)
1/v
(mM/min-1
HISTC
with ibuprofen
(mM/min-1
0.5
1.0
1.5
2.5
3.5
2.00
1.00
0.667
0.400
0.286
of PGG2)
(mM/min)
23.5
32.2
36.9
41.8
44.0
0.0426
0.0311
16.67
25.25
30.49
37.04
38.91
0.0600
0.0396
0.0328
0.0270
0.0257
Scho
0.0271
0.0239
0.0227
Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine Km and
Vmax in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units
UCR
for all numerical answers.
A. Km (no ibuprofen) =
B. Vmax (no ibuprofen) =
F, 2/18- The Spanish Voyages of Exploration, 1492-1522](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fcaf61854-7522-4075-8af1-447956fb28ae%2Fba3e75b3-e475-44a4-accd-4d3b6bfa1636%2Fjzs1mks_processed.jpeg&w=3840&q=75)
Transcribed Image Text:(This is taken mainly from Lehninger Principles of Biochemistry, 6th ed., ch. 6, #12, p. 239.)
The kinetic data below are for the cyclooxygenase reaction catalyzed by prostaglandin H2 synthase
("COX"), producing prostaglandin G2, the precursor of other prostaglandins and of thromboxanes.
Velocity was measured as a function of the concentration of the substrate arachidonic acid in the
absence and in the presence of 10 mg/ml ibuprofen (C13H1802). The molecular mass of the
undissociated acid form of ibuprofen is 206 g/mol. (Structure of the anionic form is on p. 846 of
Lehninger Principles of Biochemistry, 6th ed.) The reciprocals of velocity and of [substrate] have
been calculated for you!
IOLC
[arachidonic acid]
(mM)
1/[S]
(mM)"
velocity (rate
of formation
1/v
Velocity with
10 mg/ml
ibuprofen
(mM/min)
1/v
(mM/min-1
HISTC
with ibuprofen
(mM/min-1
0.5
1.0
1.5
2.5
3.5
2.00
1.00
0.667
0.400
0.286
of PGG2)
(mM/min)
23.5
32.2
36.9
41.8
44.0
0.0426
0.0311
16.67
25.25
30.49
37.04
38.91
0.0600
0.0396
0.0328
0.0270
0.0257
Scho
0.0271
0.0239
0.0227
Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine Km and
Vmax in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units
UCR
for all numerical answers.
A. Km (no ibuprofen) =
B. Vmax (no ibuprofen) =
F, 2/18- The Spanish Voyages of Exploration, 1492-1522
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