This is the graph for gel filtrationchromatography. Can you please explain whythe graph has the maximum and minimumpeak and relate it with the gel filtrationconcept such as the volume of elution,molecular weight, and so on. Thank you .Graph for Result 1: (Volume of elution (mL) with 1% bromophenol blue)Volume of Elution (mL) vs Corrected OD1.7891.81.61.41.20.7450.80.4770.60.379.400.4520.4060.3930.254d28 0.250.49:1280.1610.1150.20.06026.10121416Volume of Elution (mL)Corrected OD

Gel filtration chromatography is a variant of size exclusion chromatography that can be employed to…

This is the graph for gel filtration chromatography. Can you please explain why the graph has the maximum and minimum peak and relate it with the gel filtration concept such as the volume of elution, molecular weight, and so on. Thank you .

This is the graph for gel filtration chromatography. Can you please explain why the graph has the maximum and minimum peak and relate it with the gel filtration concept such as the volume of elution, molecular weight, and so on. Thank you .

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter31: Introduction To Analytical Separations

Section: Chapter Questions

Problem 31.23QAP

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Question

This is the graph for gel filtration
chromatography. Can you please explain why
the graph has the maximum and minimum
peak and relate it with the gel filtration
concept such as the volume of elution,
molecular weight, and so on. Thank you .
Graph for Result 1: (Volume of elution (mL) with 1% bromophenol blue)
Volume of Elution (mL) vs Corrected OD
1.789
1.8
1.6
1.4
1.2
0.745
0.8
0.477
0.6
0.379.400.452
0.406
0.393
0.254
d28 0.25
0.4
9:128
0.161
0.115
0.2
0.060
2
6.
10
12
14
16
Volume of Elution (mL)
Corrected OD

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