Tissue engineered cartilage derived from autologous nasal chondrocytes will be used to treat a patient with a patellar cartilage defect. 248 mg of nasal cartilage was harvested from the septum of the patient. Chondrocytes were isolated by overnight digestion in 5 mL of 0.3 % collagenase w/v per 100 mg of nasal septum. The nasal chondrocytes were then cultured in tissue culture flasks for 2 weeks, after which time they were harvested from the tissue culture flasks. The nasal chondrocyte cell suspension was centrifuged, the cell pellet resuspended in 4 mL and a cell count was taken. The total cell number obtained was 8 x 106 chondrocytes, of which 96 % were viable. What volume of 0.3% collagenase (mL) is required to digest the nasal cartilage (to 1 decimal place)? Number How much collagenase will you need (mg) to digest all of the collected cartilage (to 1 decimal place)? [¹ Number In order to begin the tissue engineering process, 500 000 cells are seeded onto a Hyalograft C scaffold What is the density of viable cells in the nasal chondrocyte suspension [cells/mL]? * Number What volume (L) of nasal chondrocyte cell suspension should be added to the Hyalograft C scaffold (to 1 decimal place? [ Number
Tissue engineered cartilage derived from autologous nasal chondrocytes will be used to treat a patient with a patellar cartilage defect. 248 mg of nasal cartilage was harvested from the septum of the patient. Chondrocytes were isolated by overnight digestion in 5 mL of 0.3 % collagenase w/v per 100 mg of nasal septum. The nasal chondrocytes were then cultured in tissue culture flasks for 2 weeks, after which time they were harvested from the tissue culture flasks. The nasal chondrocyte cell suspension was centrifuged, the cell pellet resuspended in 4 mL and a cell count was taken. The total cell number obtained was 8 x 106 chondrocytes, of which 96 % were viable. What volume of 0.3% collagenase (mL) is required to digest the nasal cartilage (to 1 decimal place)? Number How much collagenase will you need (mg) to digest all of the collected cartilage (to 1 decimal place)? [¹ Number In order to begin the tissue engineering process, 500 000 cells are seeded onto a Hyalograft C scaffold What is the density of viable cells in the nasal chondrocyte suspension [cells/mL]? * Number What volume (L) of nasal chondrocyte cell suspension should be added to the Hyalograft C scaffold (to 1 decimal place? [ Number
Chapter10: Reconstitution Of Powdered Drugs
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![Tissue engineered cartilage derived from autologous nasal chondrocytes will be used to treat a patient with a patellar
cartilage defect. 248 mg of nasal cartilage was harvested from the septum of the patient. Chondrocytes were isolated
by overnight digestion in 5 mL of 0.3% collagenase w/v per 100 mg of nasal septum. The nasal chondrocytes were
then cultured in tissue culture flasks for 2 weeks, after which time they were harvested from the tissue culture flasks.
The nasal chondrocyte cell suspension was centrifuged, the cell pellet resuspended in 4 mL and a cell count was
taken. The total cell number obtained was 8 x 106 chondrocytes, of which 96 % were viable.
What volume of 0.3% collagenase (mL) is required to digest the nasal cartilage (to 1 decimal place)?"
Number
How much collagenase will you need (mg) to digest all of the collected cartilage (to 1 decimal place)? [¹
Number
In order to begin the tissue engineering process, 500 000 cells are seeded onto a Hyalograft C scaffold
What is the density of viable cells in the nasal chondrocyte suspension [cells/mL]?
Number
What volume (L) of nasal chondrocyte cell suspension should be added to the Hyalograft C scaffold (to 1
decimal place? [
Number](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F112bd62e-8d0e-47ef-bb36-900b6b39b7ef%2F8ca9c49f-4f2d-4cdf-ac24-102d1dd165a0%2Fova6gyn_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Tissue engineered cartilage derived from autologous nasal chondrocytes will be used to treat a patient with a patellar
cartilage defect. 248 mg of nasal cartilage was harvested from the septum of the patient. Chondrocytes were isolated
by overnight digestion in 5 mL of 0.3% collagenase w/v per 100 mg of nasal septum. The nasal chondrocytes were
then cultured in tissue culture flasks for 2 weeks, after which time they were harvested from the tissue culture flasks.
The nasal chondrocyte cell suspension was centrifuged, the cell pellet resuspended in 4 mL and a cell count was
taken. The total cell number obtained was 8 x 106 chondrocytes, of which 96 % were viable.
What volume of 0.3% collagenase (mL) is required to digest the nasal cartilage (to 1 decimal place)?"
Number
How much collagenase will you need (mg) to digest all of the collected cartilage (to 1 decimal place)? [¹
Number
In order to begin the tissue engineering process, 500 000 cells are seeded onto a Hyalograft C scaffold
What is the density of viable cells in the nasal chondrocyte suspension [cells/mL]?
Number
What volume (L) of nasal chondrocyte cell suspension should be added to the Hyalograft C scaffold (to 1
decimal place? [
Number
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