Transferrin is an iron-transport protein found in blood. Desferrioxamine B is an iron chelator used to treat patients suffering from iron overload. Both are colourless in the absence of iron. The following data was obtained in aqueous solution using a 1.00 cm path length cell for both species when fully complexed with iron: Molar absorptivity M-1 cm1 A (nm) iron-transferrin iron-desferrioxamine B 428 3540 2730 470 4170 2290 Calculate the absorbance at 470 nm of a solution that is 11.0 p M in iron-transferrin and 69.5 p M in iron-desferrioxamine B, assuming both are fully complexed and that the cell path length is 1.00 cm. O A. 0.412 О в. 0.206 O.0.103 O D.0.0206

Transferrin is an iron-transport protein found in blood. Desferrioxamine B is an iron chelator used to treat patients suffering from iron overload. Both are colourless in the absence of iron. The following data was obtained in aqueous solution using a 1.00 cm path length cell for both species when fully complexed with iron: Molar absorptivity M-1 cm1 A (nm) iron-transferrin iron-desferrioxamine B 428 3540 2730 470 4170 2290 Calculate the absorbance at 470 nm of a solution that is 11.0 p M in iron-transferrin and 69.5 p M in iron-desferrioxamine B, assuming both are fully complexed and that the cell path length is 1.00 cm. O A. 0.412 О в. 0.206 O.0.103 O D.0.0206

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 14.19QAP

See similar textbooks

Similar questions

kindly answer this problem and follow this format GIVEN REQUIRED SOLUTION and write regibly thank you so much For the adsorption of a substance A from aqueous solution by charcoal at25oC the freundlich constants are n=3.0 and k=0.50 for y in grams per gramand C in grams per liter. What weight of a W is adsorbed by 2 g of charcoalfrom 1 L of a solution containing originally 2 g of the substance?
b. Given that the cuvette path length was 1.17 cm, use the slope of this line to calculate the molar absorptivity of the cobalt complex at this wavelength. (Assume Beer’s law is obeyed.) c. An unknown cobalt sample was treated as follows: 40.8 mg of the unknown was dissolved in solvent to prepare 50.00 mL of solution in a volumetric flask. 4.00 mL of this solution was treated with thiocyanate, and diluted to a final volume of 50.00 mL. A sample of this final solution was placed in the same cuvette as the standards, and showed an absorbance of 0.38 at 625 nm. Determine the percentage of cobalt in the original unknown, given that the atomic mass of cobalt is 58.933 g/mol.
You want to determine the amount of iron(III) in acomplex salt. You make a 100.0 mL solution of the complex salt by dissolving 0.0670 g of complex salt with water to the mark on a 100.0 mL volumetric flask. You get an absorbance reading for the iron from the spectrometer of 0.334. The molar absorptivity is determined to be 17605 L/mole∙cm and the pathlength is 1.00 cm. Determine the molarity of iron(III) in solution. Determine the mass percentage of iron(III) in the complex salt.
Find the isoelectric and zwitter ion of lysine, show the steps
Which are true for polarography? I Polarography is amperometry conducted with a dropping Hg electrode. II The diffusion current in the plateau region of the polarographic wave is proportional to the concentration of the analyte and is used for quantitative analysis. III The half-wave potential for maximum current is characteristic of a given analyte in a given medium and can be used for qualitative analysis for the analyte. IV Fresh Hg drops give reproducible results. V Polarography is used to primarily to study oxidations Hg may reduce when studying reductions. A.I, III, and IV B. II, III, and V C. I and V D. III, IV, and V E. II, III, and IV
Molar absorptivities of Nickel and Cobalt with 8-quinilinol are εCo = 3529 and εNi = 3228 at 365 nm, and εCo = 428.9 and εNi = 0 at 700 nm. Calculate concentration of nickel and cobalt in solution that gave Abs = 0.617 at 365 nm and Abs = 0.0235 at 700 nm
A calibration curve for the colorimetric determination of phosphorous in urine is prepared by reacting standard solutions of phosphate with molybdenum(VI) and reducing phosphomolybdic acid complex to produce the characteristic blue color. The absorbance (A) is plotted against the concentration of phosphorous. From the following data, determine the linear least-squares straight line and calculate the concentration of phosphorous in the urine sample. Phosphorous (ppm) 1.00 2.00 3.00 4.00 Unknown urine sample Fluorescence intensity 0.205 0.410 0.615 0.820 0.625
Molar absorptivities of Nickel and Cobalt with 8-quinilinol are εCo = 3529 and εNi = 3228 at 365 nm, and εCo = 428.9 and εNi = 0 at 700 nm. calculate concentration of nickel and cobalt in solution that gave Abs=0.617 at 365nm and Abs=0.0235 at 700nm.
(d) By using the data provided below, calculate the PillingBedworth ratio for the nickel (Ni) – nickel oxide (NiO) system and determine whether NiO is protective against corrosion. Atomic mass of nickel = 58.7 g.mol-1 Density of nickel = 8.91 Mg.m-3 Molecular mass of nickel oxide = 74.7 g.mol-1 Density of nickel oxide = 6.67 Mg.m-3 (e) A stainless steel pressure vessel that has fracture toughness, K1C, of 197 MN.m-3/2 is going to be exposed to a stress of 398 MN.m-2 . By using this information explain how designers can determine the thickness of the vessel so that catastrophic failure can be avoided.
Ca+2 = 36.8mg/L Mg+2 = 2.9mg/L HCO3 = 98mg/L pH = 7.53 Temp = 9 degree Celsius Calculate the following: a. Hardness in units of mg/L as CaCO3 b. Ionic strength (be sure to include H++ and OH--) c. PCO2 in equilibrium with the sample d. Saturation index with respect to calcite (SIC) e. Saturation index with respect to dolomite (SID)
A 25 ml solution of unkown containing Fe+3 and Ca+2 required 16.06 ml of 0.051 M EDTA for complete the reaction. A 50 ml of the same of the unkown was treated with NH4F to protect Fe+3. then the Ca+2 was reduced and masked by addition thiourea. upon addition of 25 ml of 0.051 M EDTA, the Fe+3 was liberated fron its floride complex and formed EDTA complex . the excess ETDA required 19.77 ml of 0.01883 M pb+2 to reach the end point by using xylenol orange indicator. Find the concentarion of Ca+2 in the unkown sample?
For spectrophometric method to be viable, which of the following must be met? a. A high MW complex must be produced in the reaction b. Analyte must have high absorptivity c. Species must emit light d. A complex with intense color must be produced