Two components in an HPLC separation have retention times that differ by 22 s. The first peak elutes in 10.5 min and the peak widths are approximately equal. Calculate the minimum number of theoretical plates needed to achieve an acceptable resolution

Q: 4. On a GC column with L= 30 m, a reference sample elutes in 13.1 min with w1/2 = 15 sec. What is…

A: “Since there are multiple questions and it is not mentioned that which one has to be solved so I…

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A: An inductively coupled plasma is abbreviated as ICP. This is a type of plasma source in which…

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A: First the value of K is calculated by using the following equation.

Q: In reversed phase HPLC, how will the retention time of a component of a mixture vary with the…

A: Retention time is a measure of the time that is taken by a solute to pass through a column.In…

Q: 1. Why do we use relative and not absolute retention times to characterize separated components?…

A: Retention time refers to the time that is spent by the solute in the stationary phase. The greater…

Q: C. >) Order the compound A, B and C from lowest to highest Rr. The sample is spotted on a silica gel…

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Q: Substances A and B have retention times of 6.40 and 7.20 min, respectively, on 15.0 cm liquid…

A: (a) The column capacity factor or say the retention factor is an important experimental quantity…

Q: A peak with a retention time of 10 minutes has a width at half-height of 7.6 s neighboring peak is…

A: given values from the above queston are ]- retention time for component 1= tr1 = 15sec. retention…

Q: Adenine and caffeine have retention times of 15.50 and 17.63 min, respectively, on a 30.0-cm column.…

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Q: Substances A and B have retention times of 12.75 and 14.28 min, respectively, on a 30.0-cm column.…

A: The formula to calculate resolution is, RS=2tB-tAWA+WB where, tA and tB are the retention time for…

Q: What characteristic makes a photodiode array spectrophotometer suitable for measuring the spectrum…

A: Ans: 1- Characteristics The following characteristic makes a photodiode array spectrophotometer…

Q: What would happen to the retention time of a compound if the followingchanges were made?a. Decrease…

A: Chromatography is the technique to separate components of a mixture.

Q: föllowing analysis of a compounds (A-D) by were obtaine HPLC: Peak Retention time (min) Width of…

A: The full form of HPLC is high performance liquid chromatography. It is used to identify or separate…

Q: A mixture of species of A, B, and C is injected into a liquid chromatographic column with length of…

A: Selectivity  factors measure as the distance between two chromatography peak. Retention  factor (k)…

Q: Methanol and ethanol are separated in a capillary GC column with retention times of 370 and 385 s,…

A: The solution of the question is given below:

Q: What is the HETP of a column that is 200mm with a retention time of 5mins. The (effective)…

A: To calculate HETP and separation factor, α

Q: PART A: Substance A was found to have a retention times of 3.15 minutes on a 70.00-cm column. An…

A: Hello. Since the question contains multiple subparts, the first three parts are solved. In case the…

Q: A sample that contained two components was separated on a column. The column had a length of 15.0…

A: Given: length of column = 15 cm initial resolution = 1.2 required resolution = 2.0 we have to find…

Q: A retention time of 85 s is obtained from a gas-chromatographic peak. The base width from the…

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Q: A mixture of A and B is injected into a liquid chromatographic column with length of 24.8 cm. The…

A: Given :  Separation resolution between A and B can be calculated using formula: Rs = 2RtB-RtAw1 +…

Q: Which of the following factors DOES NOT affect retention time of a sample undergoing normal phase…

A: Retention time is the time period where a sample spend on column. Here we have to tell which factor…

Q: What are the disadvantages of uses the Thick layer stationary phase in Thin layer Chromatography…

A: Thin layer chromatograpy is one of the conventional technique for the separation of mixture of…

Q: In a HPLC separation of two compounds, A and B, the retention times observed are 3.5 min. for A and…

A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…

Q: How does the retention time of a compound change with separation conditions? Is this change linear…

A: The interval between the injection of a sample and the detection of substances in that sample is…

Q: SUGGEST REASONABLE CLASSIC COLUMN DIMENSIONS FOR CHROMATOGRAPHING 0.5G OF SAMPLE ON ALUMNIA

A: The size of the column decide how much accurate is the separated product. The larger and wider size…

Q: In order to obtain better separation of the mixture, the above analysis was repeated using an HPLC…

A: HPLC is based on the principle where the sample (analyte) is distributed between a mobile phase…

Q: A mixture of A and B is injected into a liquid chromatographic column with length of 45 cm. The…

A: given- length of the plate= L = 45 cm plate height = H = ?

Q: Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm diameter and a…

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Q: 2. In UV and visible spectrophotometry, the specimen is generally dissolved in a solvent, and…

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Q: i) Determine the adjusted retention times for substance x and y. ii) Calculate the retention factors…

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Q: A solution containing 3.47 mM of copper and 1.73 mM of a standard gave peak areas of 3473 and 10…

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Q: The table contains data for a liquid chromatography separation of four compounds, A, B, C, and D, on…

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Q: Question attached

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Q: What is the retention factor of a sample when after injectiion the void time (tM) took 4.18 sec? The…

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Q: chromatogram below show a separation of a mixture of 2 components injected in a 25

A: The separation technique in which the liquid is a mobile phase where the molecules and sample ions…

Q: Resolution of neighboring peaks is the difference in retention time divided by the average peak…

A: Welcome to bartleby !  Introdduction :  We have to tell whether the statement is true or not .

Q: Which is NOT a factor that affects retention time? I. the pressure used II. The nature of the…

A: the retention time will vary depending on: the pressure used (because that affects the flow rate…

Q: How GC retention time can be affected by the mobile phase flow rate and the temperature of the…

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Q: Substances A and B have retention times of 16.40 and 17.63 min, respectively, on a 30.0-cm column.…

A: GIVEN: Retention times of A and B are 16.40 and 17.63 min. Peak width for A and B are 1.11 and 1.21…

Q: The following data are for a liquid chromatographic column: Length of Packing 24.7 cm Flow rate…

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Q: What is the retention factor of a sample when after injectiion the void time (tM) took 4.73 sec? The…

A: Answer: Retention factor is equal to the ratio of difference (between retention time and void time)…

Q: In HPLC, fill in the blanks with the choices below: - chromatogram retention time void volume…

A: To fill in the blanks for HPLC:

Q: The following data are for a GLC column: L= 35.5 cm, flow rate 0.303 mL/min A chromatogram of a…

A: Hello. Since the question contains multiple subparts, the first three parts are solved. In case the…

Q: Modes of Separation Normal-phase chromatography Reversed-phase chromatography Ion-exchange…

A: Modes of separation Mobile phase  stationary phase  Analyte / Separation  Normal phase…

Q: In a chromatographic analysis of an unknown mixture, compound A has a retention time of 5.56 minutes…

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Q: culate the selectivity factor (a) for the peak pairs of 1,2 and 3,4 and 5,6 in the chromatogram…

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Q: In a HPLC separation of two compounds, A and B, the retention times observed are 3.5 min. for A and…

A: Length of the column = 15 cm  Retention time for A  = 3.5 min Retention time for B  = 4.5 min

Q: 1. Methanol and ethanol are separated in a capillary GC column with retention times of 370 and 385…

A:

Q: A student spots an unknown sample on a TLC plate and develops it in dichloromethane solvent. Only…

A: TLC is thin-layer chromatography which involves distribution of a solid sample by solid-liquid…

Q: Consider the separation of components A and B on a GC column. The retention time for component A is…

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Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.