A solution containing 3.47 mM of copper and 1.73 mM of a standard gave peak areas of 3473 and 10 222, respectively in a chromatographic separation. Calculate the response factor.

Q: A chromatographic analysis is performed with a sample containing toluene and benzene. The column…

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Q: Internal standard. A mixture containing 12.8 mM analyte (X) and 44.4 mM standard (S) gave…

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Q: explain what a response ratio is in Gas Chromatography and why it might be needed to quantify…

A: Gas chromatography (GC) is an analytical technique used to separate the chemical components of a…

Q: 3. Internal Standard. A solution containing 3.47 mM X (analyte) and 1.72 mM S (standard) gave peak…

A: Response factor is obtained from are of analyte peak and standard peak and concentration of analyte…

Q: Chromatographic analysis using gas-liquid chromatography with a 40-cm packed column gave the…

A: Given,             Compound                                Retention time, min…

Q: 1. Why do we use relative and not absolute retention times to characterize separated components?…

A: Retention time refers to the time that is spent by the solute in the stationary phase. The greater…

Q: The retention factor for analytes in a sample lie between 1 and 5 why?

A: The analyte is the substance to be separated during chromatography. It is also normally what is…

Q: A chromatogram from a certain column has a peak with a w1/ 2 of 12 mm and a tr of 650 mm as…

A: Given, Width of half peak height , w12= 12 mm retention time , tr = 650 mm

Q: An analyte is found to elute from a reversed-phase column with a retention factor of 12.5 when a 20%…

A: Given data  :- At 20% ethanol and 80% water concentration retention factor = 12.5

Q: 1. Calculate the difference in retention volumes for solutes A and B that are passed through a…

A: Chromatography is the technique for the separation of a chemical mixture. The mixture may be…

Q: What effect will the following factors have on a chromatographic separation? (a) Too strong an…

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: How many theoretical plates produce a chromatography peak eluting at 12.83 min with a width at…

A: The no. of theoretical plates can be calculated as: Where N is the no. of theoretical plates, tr is…

Q: Consider a separation performed on a 50.0 mm long open tubular column with a 0.50 mm diameter and a…

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Q: In a normal-phase liquid chromatographic column, a solute was found to have a retention time of 26.7…

A: Introduction: Chromatography is a technique used to separate the mixture if solution. There are two…

Q: A student researcher performed a chromatographic separation of caffeine and aspartame with methanol…

A: The retention time of methanol (tM), caffeine (tC) and aspartame (tA) is 34.9 s, 92.8 s and 153.5 s,…

Q: According to HPLC analysis by internal standard addition the calibration equation is as follows: y =…

A: Introduction: HPLC stands form High Performance Liquid Chromatography or High Pressure Liquid…

Q: Species A and B were separated using column chromatography. The elution time for species A (1) was…

A: The theoretical plate height H = L/N where L is the length of the column and N = (tr)2/(w/4)2 where…

Q: 16. In this chromatographic technique the mobile should consists of buffer solution or with known…

A: The mobile phase of Ion Exchange Chromatography (IEC) is usually a buffer solution or a pH known…

Q: Which of these is NOT an electrochemical measurement technique? O chronopotentiometry column…

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Q: Consider the peaks for heptane (14.56 min) and p-difluorobenzene (14.77 min) in the chromatogram.…

A: a.

Q: Analysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min…

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Q: A student researcher performed a chromatographic separation of caffeine and aspartame. The retention…

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Q: density

A: According to the question, the readings are: 0.24, 0.35, 0.67, 0.89. We need to determine the…

Q: 2. Different marker inks were analyzed using a simple paper chromatography experiment. Two marker…

A: To calculate the retention factor of each spot from A to E.

Q: Describe the procedural steps to achieve adequate separation of a homogeneous, liquid mixture…

A: Polarity : Benzoic acid > Phenol > propyl benzene. Benzoic acid is having polar COOH group.…

Q: A single analysis using chromatography produced a chromatogram with three peaks. The peak for…

A: Since you have asked a question with multiple sub-parts, we will solve first three sub-parts for…

Q: The separation of APAP-L,(12 = 0.28 min) from APAP-D (W12 = 0.35 min) on a chiral stationary phase…

A: APAP-L W12 = 0.28 min has a retention time of 4.78 min and APAP-D W12 = 0.35 min has aretention…

Q: Explain the following terms used in chromatography i) Dead time ii) Number of theoretical plates

A: Several factors contributes towards the separation efficiency of any given column in chromatography…

Q: For a chromatographic peak, what is the value of retention time of analyte tR if retention time of…

A: Correct Option :  A.   75.15 s

Q: Retention volume in a chromatographic experiment 1) increases with decreasing length of the column…

A: Retention volume VR is the volum of eluent carrier gas admitted to the column between the injection…

Q: 1a) Hexane, CH3(CH2)4CH3, is found to have a long retention time when separated from a mixture. A…

A: A) Hexane is a non-polar molecule. It will easily dissolve in the non-polar solvent or have higher…

Q: A chromatographic peak has a retention time of 120 s. the width at the base of the peak equals 3.2s,…

A: Gas chromatography is an analytical technique used for the separation of chemical components of a…

Q: A student used thin layer chromatography to confirm whether he could successfully separate two…

A: TLC means “Thin Layer Chromatography”. This experiment is done on Wattman paper. If the molecule has…

Q: If retention times found on a chromatogram are 0.60 min for CH4 (=tm), 7.0 min for pentane, 8.0 min…

A: We use the formula below to find Kovat's retention index(I): Kovat's retention index, I = 100*n +…

Q: True or False: Thin-layer chromatography yields thepositive identification of a material.…

A: Thin Layer chromatography in one of the important chromatographic technique which is most widely…

Q: Ms. Muna Al Naabi in the Analytical chemistry laboratory wanted to separate chlorophenol by liquid…

A: Number of theoretical plates and plate height can be calculated using respective formulas.

Q: Water and methanol are separated using a 1.0-m packed gas chromatography column. An unretained…

A: The linear velocity of the mobile phase is calculated with the help of equation (1) where L is the…

Q: Chromatographic analysis using gas-liquid chromatography with a 40-cm packed column gave the…

A:

Q: The table contains data for a liquid chromatography separation of four compounds, A, B, C, and D, on…

A: Compound Retention time (min) Width at the Base (min) A 5.44 0.439 B 13.3 1.09 C 14.2 1.18 D…

Q: Substances A, B, C and D were found to have retention times of 8.3 min (A), 10.6 min (B), 12.4 min…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three subparts…

Q: Sample contains (A, B, C, D) analyzed by thin layer chromatography using solvent system X the result…

A: Thin-layer chromatography has two phases a stationary phase and a mobile phase. The silica gel…

Q: chromatogram below show a separation of a mixture of 2 components injected in a 25

A: The separation technique in which the liquid is a mobile phase where the molecules and sample ions…

Q: What effect will the following factors have on a liquid chromatographic separation?

A: Liquid chromatography is defined as a technique which is used to separate a sample into its…

Q: Two components of a 12-mg sample are adequately separated by chromatography through a column 1.5 cm…

A: Given: 1) There are two components of a 12-mg sample = Small column mass 2) The column is 1.5 cm in…

Q: Consider a separation performed on a 40.0 mm long open tubular column with a 0.50 mm diameter and a…

A: Compound A eluted at 12.51 min; Compound B eluted at 13.37 min; unretained solvent eluted at 1.145…

Q: How we optimize chromatographic separations?

A: Chromatographic separation of mixture of various compounds are based on their distribution between a…

Q: In a chromatographic analysis of an unknown mixture, compound A has a retention time of 5.56 minutes…

A:

Q: A mixture of three components is separated using paper chromatography, and each component is…

A: Given: Absorbance of standard solution- Compound A = 0.72 Compound B = 0.55 Compound C = 0.80 For…

Q: . A third spectrophotometric method for the quantitative analysis of Pb in blood yields an Ssamp Of…

A: Concentration of Pb2+ in original sample can be calculated using standard addition method. In which…