Understand the difference between DF and concentration! What is your dilution factor if you have added 8 ml buffer to 1 ml bacterial culture? Round to nearest whole number. The dilution factor is What is your dilution factor if you have added 8 ml of water to 1 ml of 50XTBE buffer? How many times have you diluted your 50XTBE? Round to nearest whole number. The dilution factor is I have diluted my 50x TBE times. What is the concentration of your diluted solution if you added 8 ml of water to 1 ml of 50XTBE buffer? Round to nearest whole number. The concentration of the dilution is X.

Understand the difference between DF and concentration! What is your dilution factor if you have added 8 ml buffer to 1 ml bacterial culture? Round to nearest whole number. The dilution factor is What is your dilution factor if you have added 8 ml of water to 1 ml of 50XTBE buffer? How many times have you diluted your 50XTBE? Round to nearest whole number. The dilution factor is I have diluted my 50x TBE times. What is the concentration of your diluted solution if you added 8 ml of water to 1 ml of 50XTBE buffer? Round to nearest whole number. The concentration of the dilution is X.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Titration 1 Titration 2 Titration 3 Volume of HCl solution (mL) 5.0 ml 5.0 ml 5.0 ml Initial buret reading (mL) 0.5 ml 0.5 ml 0.5 ml Final buret reading (mL) 45.61 ml 45.56 ml 45.64 ml Volume of NaOH used (mL)
1Upon the addition of 30.00 mL of 0.350 M HCl to a 80.00-mL of the weak base amine (Kb = 4.95 x 10-10) whose concentration is 0.530M, the resulting pH is then equal to 8.80. True or False? 2.When 100 mL of a 1.0M strong acid is 50% titrated with 1.0M strong base, the volume of the base should be added is 50mL and this corresponds the equivalence point. True or False? 3. The pH of a buffer can be calculated using the formula: 14-pOH = pKb + log of [proton acceptor]/[acid component]. True or False?
Construct a curve for the titration of 20.00 mL 0.500 N succinic acid (HO2CCH2CH2CO2H) by 0.1000 N NaOH solution. Use MS Excel for convenience. Titrant volumes: 0.00 mL, 5.00 mL, 10.00 mL, 15.00 mL, 20.00 mL, 25.00 mL, and 30.00 mL. (CH2)2(CO2H)2⇄(CH2)2(CO2H)(CO2)^- +H^+ Ka1= 6.21 x 10^-5; pKa1 =4.207 (CH2)2(CO2H)(CO2)^-⇄(CH2)2(CO2)2^2-+ H^+ Ka2= 2.31 x 10^-6; pKa2 =5.636
A 1.0-LL buffer solution initially contains 0.30 molmol of NH3NH3 (Kb=1.76×10−5)(Kb=1.76×10−5) and 0.30 molmol of NH4ClNH4Cl. What mass of the correct reagent should you add? Express your answer using two significant figures.
Suppose a solution of NaOH was titrated and found to have a molarity of 0.250 M. This solution is now used to titrate 25.00 mL of an unknown H2SO4 solution: H2SO4(aq) + 2NaOH(aq) -> 2H2O(l) + Na2SO4(aq) If 10.00 mL of the NaOH solution were needed to reach the phenolphthalein endpoint a) how many moles of NaOH were used in the titration? b) how many moles of H2SO4 were neutralized? c) what is the molarity of the unknown H2SO4 solution?
Indicate using true or false for each of the following statements: 7. The pH of a solution containing ephedrine 0.1 M and ephedrine HCI 0.01 M? (Kb for ephedrine = 2.3 x 10-5) is 7.99. 8. The maximum buffer capacity for solutions can be achieved when the required pH double than pKa of the acid.. 9. When one of the buffer components is depleted completely the solution lose its buffering capacity and can no longer resist the change in pH. 10. At a hydrogen ion concentration of 1.75 x 10^-5, what is the capacity of a buffer containing 0.10 mole each of acetic acid and sodium acetate per liter of solution? (Ka = 1.75 x 10^-5 ) is 9.11.
Many of the Western blot immunodetection buffers are based on a buffer solution abbreviated TBS-T. Tris Buffered Saline-Tween (TBS-T) 50 mM Tris-Cl, pH 7.6 150 mM NaCl 0.1% Tween-20 note that Tris and NaCl are crystals but Tween-20 is a detergent (similar to SDS but neutral) that is bought as a concentrate of 10% and will foam if stirred excessively We will need about 700 ml of TBS-T overall. Write out exactly how you would make this solution.
Mola and Rity were tasked to analyze the acidity of a 10.0-mL fermented milk sample diluted with water in a 250-mL volumetric flask. Mola obtained a 15.0-mL aliquot of this solution, diluted it to 50.0 mL, and then titrated this diluted sample. Titration data were given to Rity and she found out that the 50.0-mL diluted sample contains 0.21 mmol of lactic acid. MW: Lactic Acid (90.08) Follow proper significant figure rulesa. What is the lactic acid concentration (in M) of the titrated sample? b. Determine the lactic acid content (in M) of the 10.0-mL fermented milk sample. c. What is the lactic acid concentration (in %w/v) of the 10.0-mL fermented milk sample?
Mola and Rity were tasked to analyze the acidity of a 10.0-mL fermented milk sample diluted with water in a 250-mL volumetric flask. Mola obtained a 15.0-mL aliquot of this solution, diluted it to 50.0 mL, and then titrated this diluted sample. Titration data were given to Rity and she found out that the 50.0-mL diluted sample contains 0.21 mmol of lactic acid. What is the lactic acid concentration (in M) of the titrated sample?
Mola and Rity were tasked to analyze the acidity of a 10.0-mL fermented milk sample diluted with water in a 250-mL volumetric flask. Mola obtained a 15.0-mL aliquot of this solution, diluted it to 50.0 mL, and then titrated this diluted sample. Titration data were given to Rity and she found out that the 50.0-mL diluted sample contains 0.21 mmol of lactic acid. MW: Lactic Acid (90.08) a. What is the lactic acid concentration (in M) of the titrated sample? b. Determine the lactic acid content (in M) of the 10.0-mL fermented milk sample. c. What is the lactic acid concentration (in %w/v) of the 10.0-mL fermented milk sample?
What volume of 0.12 M HCl must be added to 1.5 g glycine amide to make buffer at pH 8.5? What volume of 0.08 M NaOH must be added to 1.1 g glycine amide hydrochloride to get a pH of 7.5?Suppose you want to make 250 mL 0.0700 M glycine buffer at pH 8.00. What mass of glycine amide and glycine amide HCl are needed? Hint: you need 2 eqns w/ 2 unknowns. Use the mb eqn and HH eqn. What mass of glycine amide and what volume of 0.400 M HCl are needed? What mass of glycine amide HCl and what volume of 0.600 M NaOH are needed?