could you elaborate more on the process of step 2? it isn't explained vey well Get live help whenever you need from online tutors! Try bartleby tutor today >Q SEARCH& ASK AN EXPERTA CHATVx MATH SOLVERAnenzymeis discovered that catalyzes the chemical reaction A team of motivated researchers sets out tostudy the enzyme, which they callhappyase. They find that the kcat for happyase is 600 s and carry out several additional experiments.When [Et] = 20 nM and [SAD] = 40 µM, the reaction velocity, V0, is 9.6µM s. Calculate Km for the substrate-SAD.SAD = HAPPYTranscribed Image Text: SAD = HAPPYExpert SolutionStep 1Enzymes are the biomolecules which acts as catalysts for many biochemical reactions and increases therate of reaction by decreasing the activation energy required for the reaction.Step 2In the given question, the given enzymatic reaction that is catalyzed by enzyme Happyase is:SAD = HAPPYThe given concentration of substrate [SAD] is 40 uM, and enzyme concentration is 20 nM. The Kcat forthe enzyme is 600 /sec. The rate of reaction (Vo) is 9.6 uM/sec. The Km can be calculated as follows:VтахутахTE; ]Vmax[SKm + [SКcat 3D— Кcat x| E, Vтах — 600 x 20 х 10-3 риM/secVmax12 иМ/secVoStep 3Hence, the Km for the substrate SAD is 10 µM.

Michaelis menten constant, Km is the substrate concentration required to produce half maximum…

Answered: When [Et] = 20 nM and [SAD] = 40 µM,…

Science

Biochemistry

When [Et] = 20 nM and [SAD] = 40 µM, the reaction velocity, V0, is 9.6µM s1. Calculate Km for the substrate SAD.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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could you elaborate more on the process of step 2? it isn't explained vey well

Get live help whenever you need from online tutors! Try bartleby tutor today >
Q SEARCH
& ASK AN EXPERT
A CHAT
Vx MATH SOLVER
An
enzyme
is discovered that catalyzes the chemical reaction A team of motivated researchers sets out to
study the enzyme, which they call
happyase. They find that the kcat for happyase is 600 s and carry out several additional experiments.
When [Et] = 20 nM and [SAD] = 40 µM, the reaction velocity, V0, is 9.6µM s. Calculate Km for the substrate
-
SAD.
SAD = HAPPY
Transcribed Image Text: SAD = HAPPY
Expert Solution
Step 1
Enzymes are the biomolecules which acts as catalysts for many biochemical reactions and increases the
rate of reaction by decreasing the activation energy required for the reaction.
Step 2
In the given question, the given enzymatic reaction that is catalyzed by enzyme Happyase is:
SAD = HAPPY
The given concentration of substrate [SAD] is 40 uM, and enzyme concentration is 20 nM. The Kcat for
the enzyme is 600 /sec. The rate of reaction (Vo) is 9.6 uM/sec. The Km can be calculated as follows:
Vтахутах
TE; ]
Vmax[S
Km + [S
Кcat 3D
— Кcat x| E, Vтах — 600 x 20 х 10-3 риM/secVmax
12 иМ/secVo
Step 3
Hence, the Km for the substrate SAD is 10 µM.

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