A technician investigated the effect oftemperature on the rate of an enzyme-controlled reaction. At each temperature, shestarted the reaction using the sameconcentration of substrate.15amount of37 Cproduct in11. Give one other factors the technician wouldhave controlled.1060 "C12. Explain the difference in the initial rate ofreaction at 60°C and 37°C.13. Explain the difference in the rates ofreaction at 60°C and 37°C between 20 and102030Time after start of reaction i minutes40 minutes.

The substrate molecules and catalysts undergo distinct changes in the reaction when the enzyme…

Answered: A technician investigated the effect of…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Please answer clearly and directlyAn enzyme that works best at pH 2 and temperature 35-39°C was dissolved in water then boiled. Afterwards, the substrate was added. After a while, no reaction occurred and the substrate wasn't converted to products. Explain why the result was negative assuming that the enzyme is correct for the substrate
A biochemst wants to dtermine the effect of inhibitor on a certain enzyme. See attached data. Using linaer regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor 1. y- int ( up to 5 decimal places) 2. slope 3. Vmax 4. Km Using linear regression analysis determine the values of Vmax and Km of the enzyme in the presence of inhibitor 1. y- int ( up to 5 decimal places) 2. slope 3. Vmax 4. Km
An enzyme with a Km of 3 X 10-4 M was tested at an initial substrate concentration of 10-5 M. In 1 minute, 5% of the substrate was found to be utilized. (a) What percentage of the substrate was utilized in 5 minutes? (b) If the initial substrate concentration had been 8 X 10-7 M, what percentage of the substrate would have been utilized in 5 minutes? (c) Vmax =? (d) How long would it take to utilize 50% of the substrate at 8 X 10-7 M? (e) How long would it take to utilize 75% of the substrate at 10-5 M?
A student determined the Km, Vmax and turnover number (kcat) for an enzyme by performing reactions with varying amounts of substrate and a concentration of enzyme at 10nm The student then decided to repeat the reaction with the same substrate concentration but with increased enzyme concentration to 20nm. Would the following increase, decrease or stay the same? Km Vmax Kcat
Using drawn graphs or image, xan you explain the factors like pH, temperature, substrate concentration, and enzyme concentration on its effect on the activity of an enzyme. Thank you!
What is the CORRECT sequence of events to activate an enzyme? Group of answer choices a. enzyme/product complex-enzyme/substrate complex-products leaving active site-substrate entering active site b. enzyme/product complex-products leaving active site-enzyme/substrate complex-substrate entering active site c. substrate entering active site- enzyme/product complex-enzyme/substrate complex-products leaving active site d. substrate entering active site-enzyme/substrate complex-enzyme/product complex-products leaving active site
In an enzyme experiment, when the enzyme is added only to the substrate, the Vmax value is 0.828 and the Km value is 17.76. When the substrate and activator are added to this enzyme, the Vmax is 0.964 and the Km value is 10.6066. When substrate and inhibitor are added to the enzyme, the Vmax value is 0.550 and the Km value is 11.34. Estimate the effect of the substance added. Are these substances suitable activators/inhibitors for the laccase enzyme?
Please handraw this graph with all the necessary detailed information: Imagine that I text enzyme rate for four different temperatures: 10 degrees celsius, 20 degrees celsisus, 30 degree celsius, and 40 degree celsius, in separate tubes. The enzyme appears to work faster as temperature increases, but completely ceases activity at 40 degrees celcius. Sketch a graph to show this outcome, but here you will graph product formation (nmoles/mL) vs. time (minutes). The graph should be 4 lines and HANDDRAWN. Include a legend if necessary. You do not need precise quantitivate values, but most show the correct trends on the graph.
A biologist investigating enzyme function plotted the activity of a particular enzyme (y-axis) vs pH (x-axis). Experiment A was performed at 30 oC, experiment B at 10 oC and experiment C at 100 oC. Experiments A, B, and C all used the same type of enzyme, they simple tested the enzyme's activity at different temperatures. Using your knowledge of enzyme activity, answer the questions based on the results shown in the graph. (Answer question B) Graph included B. Why is there no hill in the middle of line C?
The graph is an example of the rate of product production by an enzyme when the substrate is in excess. Theorize why the reaction rate plateaus. asap
The rate of an enzyme-catalysed reaction was measured in the absence or presence of a non-competitive inhibitor. Which of the following Lineweaver-Burk plots would you expect to see? a. (a) b. (b) c. (c) d. (d)
A biologist investigating enzyme function plotted the activity of a particular enzyme (y-axis) vs pH (x-axis). Experiment A was performed at 30 oC, experiment B at 10 oC and experiment C at 100 oC. Experiments A, B, and C all used the same type of enzyme, they simple tested the enzyme's activity at different temperatures. Using your knowledge of enzyme activity, answer the questions based on the results shown in the graph. (Answer question 1 ) Graph included 1. After conducting experiment C, the investigator cools the reaction vessel to 30 oC and repeats the experiment using the exact same enzyme (not just the same type of enzyme, but the very same enzymes that was was used in experiment C). Draw the shape of the line that you would expect to see on a graph for this new experiment.

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