What are restriction enzymes. 2. Give 5 restriction enzymes and its source. 3. What is a restriction map. 4. Explain each step used in restriction mapping. STEP 1: Preparation of DNA for restriction analysis. STEP 2: Restriction digestion of DNA. STEP 3: Separation of restricted DNA. STEP 4: Collecting data. STEP 5: Construction of restriction map

What are restriction enzymes. 2. Give 5 restriction enzymes and its source. 3. What is a restriction map. 4. Explain each step used in restriction mapping. STEP 1: Preparation of DNA for restriction analysis. STEP 2: Restriction digestion of DNA. STEP 3: Separation of restricted DNA. STEP 4: Collecting data. STEP 5: Construction of restriction map

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 6QP

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1. What are restriction enzymes.2. Give 5 restriction enzymes and its source.3. What is a restriction map.4. Explain each step used in restriction mapping.STEP 1: Preparation of DNA for restriction analysis.STEP 2: Restriction digestion of DNA.STEP 3: Separation of restricted DNA.STEP 4: Collecting data.STEP 5: Construction of restriction map
In 5 sentences only, What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.
Describe the nature of recognition sites for restriction enzymes and the nature of the ends of the DNA that are left. Why do we need to run a gel electrophoresis after enzyme digestion
Explain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.
Restriction maps illustrate the lengths of DNA fragments between restriction sites. Which of the following information can be gathered from the analysis of restriction maps? Select all that apply. a gene sequence b presence of mutations c nucleotide sequence d disease identification
Restriction enzymes and DNA ligase play essential roles in DNA cloning. How is it that a bacterium that produces a restriction enzyme does not cut its own DNA? Describe some general features of restriction sites.
On the gel shown below are four DNA samples. Samples A to C are taken from tissues of landslide victims that are being identified, while sample D came from a hair sample brought by a mother looking for the remains of her son. (see img) i. If similar band patterns in a gel are created using the same restriction enzyme, what does that tell you about the DNA sequence of the samples? ii. In sample C, only two fragments were created. How many restriction sites (regions where enzymes cut) are present in sample C?
Which statement is NOT the function of restriction enzyme? Group of answer choices It cuts the vector’s plasmid and the desired gene from the original DNA. It ligates the desired to gene to vector’s plasmid. It acts as scissors of DNA that cut along a specific sequence. It serves as degrading proteins that cut plasmid on specific target site.
1. (a) Restriction sites are usually ______. Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequences (b) Involves joining a donor DNA fragment of interest to a vector Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequences
2. Which of these statements is INCORRECT about restriction enzymes Restriction enzymes form phosphodiester bonds when DNA are assembled Palindromic sequences are recognized by restriction enzymes Restriction enzymes recognize blunt ends Restriction enzymes recognize sticky ends Restriction enzymes originated from bacteria
All of the following are performed during restriction fragment length polymorphism analysis. 1. splitting of double-stranded into single-stranded DNA 2. gel electrophoresis 3. autoradiography 4. immersion in radioactive probes 5. digestion of DNA with restriction endonucleases 6. use of a positive charge to transfer single-stranded DNA from a gel to a membrane. The correct sequence of these operations is what
Illustrate the steps in restriction digestion and PCR [ Please make it clean and readable, labelled should be included ] ( THIS IS ALL ABOUT APPLICATIONS OF RECOMBINANT DNA).