What are the advantages and disadvantages of viable cell count and the turbidimetric methods?
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What are the advantages and disadvantages of viable cell count and the turbidimetric methods?
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- What is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?What are the advantages/benefit of isometric and allometric growth on an organism?When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies on your 1 x 106 plate. How many cells were in the original sample?
- What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?If the field diameter is 2.4 mm and there are 16 cells across the field of view when using the 40X objective, what is the true length of one cell in millimeters (mm)?If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperation
- What is the application of Gel Electrophoresis in healthcare? Cite at least 2 specific examples.What are the key factors that affect the success of cell culture techniques in a laboratory setting?A 2x10-6 dilution of your bacterial culture yielded 10 colonies. What was the cell density (incells/ml) of your bacterial culture?