Order the following choices to indicate the sequence of steps in the process of elongation of a growing peptide chain. Peptidyltransferase reaction catalyzed by large rRNA subunit. Aminoacyl-tRNA binds the ribosomal A site via EF1x-GTP hydrolysis. EF2-GTP hydrolysis promotes translocation of the ribosome. Release of EF1x-GDP.

Order the following choices to indicate the sequence of steps in the process of elongation of a growing peptide chain. Peptidyltransferase reaction catalyzed by large rRNA subunit. Aminoacyl-tRNA binds the ribosomal A site via EF1x-GTP hydrolysis. EF2-GTP hydrolysis promotes translocation of the ribosome. Release of EF1x-GDP.

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter15: Genes And Proteins

Section: Chapter Questions

Problem 3VCQ: Figure 15.16 Many antibiotics inhibit bacterial protein synthesis. For example, tetracycline blocks...

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Order the following choices to indicate the sequence of steps in the process of elongation of agrowing peptide chain. a. release of EF1-GDPb. EF2-GTP hydrolysis promotes translocation of the ribosomec. peptidyltransferase reaction catalyzed by large rRNA subunitd. aminoacyl-tRNA binds the ribosomal A site via EF1-GTP hydrolysis
Consider the following tRNAs, where the numbered forms represent the amino acids associated with them, answer briefly: PICTURE Question 1: The numbering indicates the order in which these tRNAs are recruited to the A site of the ribosome. Write the sequence of the mRNA being translated in the 5' - 3' direction Question 2: What is the amino acid sequence of the produced polypeptide? Question 3: Researchers discover that a mutation is in the anticodon of the gene encoding the proline tRNA of an individual. The anticodon sequence is normally 3' GGA 5', but in this individual the anticodon sequence is 3' GGG 5'. It appears that this individual suffers no adverse consequences. How can this be? (2 response items)
Which of the following cannot be said regarding aminoacyl tRNA synthetase? It is essential for the correct reading of codons. A single tRNA synthetase can attach different amino acids to their corresponding tRNA. It is first activated when it catalyzes a reaction between ATP and an amino acid to form an aminoacyladenylic acid (amino acyl-AMP). It is responsible for the attachment of the correct amino acid to the correct tRNA. There is a different tRNA synthetase for each amino acid that will be attached to a tRNA.
Which of the following statements regarding O-linked glycosylation is FALSE? A. It occurs on asparagines (Asn) in specific proteins. B. It is an important post-translational protein modification. C. It is built sequentially on proteins by individual enzymes that are found in each cisterna of the Golgi (i.e. cis, medial, trans). D. It occurs in the Golgi. It occurs on serine (Ser) and threonine (Thr) residues in specific proteins.
For each amino acid incorporated into a polypeptide the cell has to invest three high-energy phosphates (ATP or GTP). At which step of the overall process is energy investment NOT required? What is the correct answer from below? Ester bond formation between a tRNA and an amino acid Release of an aminoacyl-tRNA into the A-site of a ribosome Translocation of a ribosome along an mRNA molecule Peptide bond formation between amino acids none of the above
Indicate whether each of the following events occurs when tryptophan is high or when tryptophan is low by placing a check in each of the appropriate blanks. Share your reasoning. Event Tryptophan high Tryptophan low Ribosome does not stall at Trp codons ____________ ____________ Region 2 of the leader pairs with region 3 ____________ ____________ Ribosome covers part of region 2 of leader ____________ ____________ Transcription is terminated before structural genes are transcribed ____________ ____________
Choose the answer that has these events of protein synthesis in the proper sequence. (Note some steps may be missing) A peptide bond forms A small ribosomal subunit binds with the mRNA. The tRNA leaves the P site, and the ribosome translocates to the P site. The Methionine initiator-tRNA binds to the start codon. An incoming amino-acyl tRNA binds to the A site The large ribosomal subunit binds the small subunit Group of answer choices A 4, 1, 2, 5, 6, 3 B 2, 4, 6, 5, 1, 3 C 1, 3, 2, 4, 5, 6 D 4, 1, 3, 2, 5, 6 E 5, 4, 3, 2, 6, 1
Once the chains of peptides that make up lysyl-tRNA synthetase protein are synthesized in ribosomes, lysyl-tRNA synthetase needs to have the proper active site in order to perform its function, explain the process of protein folding necessary to have a proper 3-D structure, include effect of thermodynamics and different states in folding, including what happen when there are prolines that form peptide bonds with other amino acids, and any disulfide bridges
Which of the following is not true? A.) A single activating enzyme can interact with all the tRNAs for its corresponding amino acid B.) The selectivity of the aminoacyl-tRNA sunthetases for their tRNA molecules is oftern called the second Genetic code C.) There are two major classes of aminoacyl-tRNA syntetases D.) Aminoacyl-tRNA synthetases link amino acids to tRNA molecules without the need for an energy source
The peptidyltransferase reaction begins with the hydrolysis or breaking of the bond between the carboxyl group of the last amino acid added to the growing polypeptide and the 3' end of the tRNA in the P site. How does the ribosome get the necessary activation energy to catalyze that hydrolysis reaction?
The synthesis of a protein requires that the amino acids that constitute the growing polypeptide chain be covalently linked to the amino acid attached to the tRNA at the aminoacyl site of the ribosome. Which of the following catalyses this reaction? Options: the aminoacyl tRNA synthetase eEF2 eEF1-GTP a large ribosomal RNA the initiator tRNA met
Number the following steps of protein synthesis in order in which they occur, starting with 1 and ending with 9. a. ____ the stop codon is reached, and the polypeptide is released b.____ the small ribosomal subunit finds the start codon, and the large ribosomal subunit joins. c.____ the end of the gene is reached, and the pre-mRNA is released and then edited. d. ____ The transcription factor bonds the promoter. e. ____ the protein is folded and modified to become functional. f. ____ RNA polymerase builds the mRNA transcript. g. ____ mRNA and initiator tRNA bind the small ribosomal subunit. h. ____ new tRNAs are brought into the A site successively, and the peptide chain of the tRNA in the P site is joined to the amino acid of the tRNA in the A site. i. ____ mRNA exits the nucleus via a nuclear pore.