Under what circumstances should the product Ocuser-pilo be used? Compare the advantages and disadvantages of using this long-acting ocular insert.
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Q: For each individual on gel, determine if the individual is homozygous for presence of the Alu…
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Q: determine if the individual is homozygous for presence of the Alu insertion(++), heterozygous (+-),…
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Step by step
Solved in 4 steps
- For each individual on gel, determine if the individual is homozygous for presence of the Alu insertion (++), heterozygous (+-), homozygous for the absence of the Alu insertion (- -), or if the results are inconclusive (I) (explain in detail what leads to your determination of each individual on the gel and why). Explain what may have caused any unexpected bands (neither 850 nor 550) lack of bands.Can native gel electrophoresis of protiens be used to determine protein molecular weight? Explain.Explain the term FAD (flavin adenine dinucleotide) ? Define the importance of FAD (flavin adenine dinucleotide) ?
- What is the isoelectric point of this fragment? DHVTHLRKMGis bradford assay using dye Coomassie Brilliant Blue G-250 a different method from using a spectrophotometer ? if so how is it different to determine protein concentration?The oligonucleotide d-ATGCCTGACT was subjected to sequencing by Sanger’s dideoxy method, and the products were analyzed by electrophoresis on a polyacrylamide. Draw a diagram of the gel banding pattern obtained.
- Using the “target DNA” sequence provided in the attached image, what sizes of inserts can be prepared using HindIII digestion? Which one contains the coding sequence?what are the advantages and disadvantages of using recombinant protein and affinity chromatography for protein purification compared to gel filtration (size exclusion chromatography) and DEAE-sepharose chromatography (ion-exchange chromatography)?In the procedure shown, why was it necessary to link thecoding sequence for the A or B chains to the sequence forβ-galactosidase? How were the A or B chains separated fromβ-galactosidase after the fusion protein was synthesized in E. coli?
