What are the possible reasons why there are no spots on the Developed Plate after analysis?

Q: Discuss the factors that cause the broadening of a chromatographic peak based on the Van Deemter…

A: Given We know about the factors that cause the broadening of a chromatographic peak based on the…

Q: In an experiment that uses TLC, like the one performed in this course, which of the following…

A: Thin layer chromatography (TLC) is used to isolate and separate non-volatile mixtures based on their…

Q: Based on the readings and lecture on emulsions, and the procedures for this lab answer the following…

A: Given dispersed phase Air Oil Water Egg Dispersed phase =…

Q: For standard turbidity measurements, the increase in signal is due to a.) higher amounts of analytes…

A: Its intensity ( scattered light ) can also be measured by this, Precipitate size and shape are…

Q: What is the benefit of using fluted filter paper instead of not folding it?

A: “Fluted “ filter paper (LEFT) is used when we wish to separate a liquid and a solid, keeping the…

Q: Which is the more effective extraction technique, single or multiple extractions? Explain.

A: Extraction is a process for separating chemicals based on differences in solubility in two…

Q: Nitrogen Content Assay Method I is also known as? Distillation Micrometric Method Macromethod…

A: Nitrogen Content Assay Method I is also known as?

Q: The key to a successful HPLC procedure is good sample separation which is represented by a clean…

A: In this question, we have to choose the correct option from the given options.

Q: What are the different melting point apparatus that are available for routine analysis? Describe…

A: A melting point apparatus measures the melting Point of a given substance.

Q: what is the difference between liquid phase extraction and solid phase extraction

A: A question based on analytical process that is to be accomplished.

Q: Why line extraction from a point cloud is needed ?

A: Planar structure extraction from point clouds is an important process in many applications.

Q: Why do we know the aqueous layer is the upper layer during the wash steps?

A:

Q: Find the Rf value from given data. Thank you

A: In Thin Layer Chromatography, the compounds can be separated by the solvents in the TLC plate. The…

Q: The solvent level in the development chamber should be above the level of the spotted compounds on…

A: The solvent level in the development chamber should be above the level of the spotted compounds on…

Q: Which is more polar, the water solvent or the chromatogrphy paper?

A: In paper chromatography, paper is made up of cellulose in which there are various hydroxyl group…

Q: answer the following questions 1. What is the purpose of flame sterilizing the inoculating loop or…

A:

Q: Of Aspirin, ethanol, acetaminophen, ethyl acetate, hexane, and water which could go undetected…

A: HPLC or high perfomance liquid chromatography is a usefull technique to separate and analyse…

Q: Which of the ff. turbidity measurements is the least accurate? Secchi Disk Method Jackson Candle…

A: Answer  All given methods are used for measurement of turbidity

Q: Three sample, X (neutral), Y (basic), and Z (acidic), were mixed and dissolved together in…

A: Dissolve the mixture in dichloromethane and separate the organic and aqueous layer using liquid…

Q: why is it necessary to turn the separatory funnel upside down

A: It is a glassware used to separate two layer namely aqueous layer and organic layer.  It contains…

Q: What would be the effect of increasing the carrier gas flow rate on the retention rate?

A: Retention time is the distance that the compound cover from the time of injection till the maxima in…

Q: 10)Examine each of the errors below, discuss how the error would affect the separation. Describe how…

A: Hello. Since the question contains more than three sub-parts, the first three sub-parts shall be…

Q: In a liquid - liquid extraction, what is preferable? Three extractions with a small volume or one…

A: Liquid-liquid extraction is also known as solvent extraction or solvent partitioning. In this…

Q: For the TLCS shown below, both plates have the exact same materials spotted on each lane (that is,…

A: Thin layer chromatography.

Q: What will be the residue and what will be formed at cooler portion of test tubes if the tube…

A: Sugar: Sugar belongs to carbohydrate, which contains energy. It is white crystalline solid with…

Q: Before applying the eluent on the column, why is it important to drain the solvent to a level right…

A:

Q: The following conditions may not cause tailing EXCEPT a. Volatile solvent b.Uncovered chamber…

A: TLC is one of the basic technique to get a preliminary idea about the compound synthesized in the…

Q: Why should the syringe be removed from the sensor when setting the initial volume for each trial?

A: Option C

Q: What error is introduced into the determination of an Rf value if the top is left off the developing…

A: In chromatographic technique, the components of a mixture are separated by using a developing…

Q: What property determines which layer is on top or bottom in an extraction?

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: Determine the Kovats retention index for all six analytes.

A: In gas chromatography, Kovats retention index is used to convert retention times into…

Q: Liquid extraction What can you do if you don't know which layer is which in an extraction procedure?…

A: Extraction is a fundamental technique used to isolate one compound from a mixture. A liquid/liquid…

Q: Diagram of Paper Chromatogram: Marker Dyes Draw all spots that appear on the chromatogram, and mark…

A: First observe the solvent front. It could be nearly 11 cm as per your picture of paper and ruler.…

Q: What error would occur if the Sep-Pak C18 cartridge (the nonpolar stationary phase) was not…

A: C-18 cartridges are columns that are filled with packing material that act as stationary phase. The…

Q: PIGMENT SPOT PHI   WORK   DUEAN   MEEN

A: The crude reaction mixture has two or more reactions combined in a different molar ratio. Generally,…

Q: After acid-base extraction why is it important to wash organic layer at least once with pure water?

A: A question based on quantitative analysis, which is to be accomplished.

Q: For the TLCS shown below, both plates have the exact same materials spotted on each lane (that is,…

A: Distance Travelled by the Sample depends  upon the type of Polar mobile phase used . IF THE mobile…

Q: what will produce a smaller Rf value if the solvent and paper are both changed?

A: Increasing the polarity of solvent will result in increase in Rf value as the compound in even for…

Q: Why must the spots be above the level of the development solvent in the developing chamber?

A: Given statement,     The spots be above the level of the development solvent in the developing…

Q: Question 5 Which part of the HPLC contains majority of the solvent? O fraction collector O reservoir…

A: The beginning of the HPLC system occurs with the solvent reservoir which contains the solvent , and…

Q: Explain briefly a proper technique to use burette

A: A question based on tools in analytical chemistry that is to be accomplished.

Q: The following conditions may not cause tailing EXCEPT a. Thick filter paper as the stationary phase…

A: Tailing in thin layer chromatography :  Due to the using high concentration of solute or other…

Q: how does the intermolecular force of attraction lead to stain removal? state reference

A: Intermolecular forces are the type of forces operating in between the atoms or ions of different…

Q: In comparing direct headspace injections with SPME headspace injections, later eluting peaks (by GC)…

A: Chromatography is a laboratory technique for separation of mixtures. The mixture is dissolved in a…

Q: Which solvent is appropriate to use in the iodoform test? Select one: Dioxane Acetaldehyde…

A: The iodoform test is used to detect the compound that contains the carbonyl group and the structure…

Q: Because water was not supposed to react with any of the reagents or indicators used in this…

A: Given that, Water was not supposed to react with any of the reagents or indicators used in this…

Q: Coarse Dispersion What will be the results after you reconstitued a cefixime 60 ml a powder for…

A: A question based on colloidal solution, which is to be accomplished.

Q: why do we have to order samples (from least concentrated -most concentrated) when measuring the…

A: A question based on qualitative analysis, which is to be accomplished.