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What error would occur if the Sep-Pak C18 cartridge (the nonpolar stationary phase) was not subjected to prewetting, and why?
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- 1.what data must be available when HPLC method is passed on 2.why eluents should be degassedThe key to a successful HPLC procedure is good sample separation which is represented by a clean peak for each individual compound. What should we do to ensure a good sample preparation? A. Use a column and a mobile phase with different polarity B. Use a column and a mobile phase with the same polarity C. Use a lower temperature higher than the mobile phase boiling point D. All of thoseIn the video (liquid-liquid extraction), why is it necessary to turn the separatory funnel upside down and to open the stopcock?
- Explain how solid-phase microextraction works. Why is cold trapping necessary during injection with this technique? Is all the analyte in an unknown extracted into the fiber in solid-phase microextraction?The following conditions may not cause tailing EXCEPT a. Volatile solvent b.Uncovered chamber c.Thick filter paper as the stationary phase d.Heavy sample spottingSilica gel is very polar stationary phase. Therefore, more polar molecule sticks to silica gel on the TLC plate and has higherRfvalue. True False
- An ideal RF value for a compound on a developed TLC plate is 0.30. How could you adjust the TLC developing conditions to obtain an RF of 0.30 if it's too low or too high (Hint: Think about chromatography phases).Explain why it is needed to filter the solvent before introducing it to the HPLC systemA student spots an unknown sample on a TLC plate. A single spot with an Rf of 0.55 showed up on the plate after developing the sample in hexanes-ethyl acetate 50:50. Does this prove that the unknown is a pure compound?
- Why is splitless injection used with purge and trap sample preparation?A small quantity of compound A was applied to the mid-point of the following TLC plate’s line of application (drawing is not to scale). After development of this plate by a suitable solvent, compound A was determined to have an Rf value of 0.22. Once the solvent had evaporated from the TLC plate it was re-developed using the same solvent as before. What distance (in mm) did compound A travel during the second development? Please carry maximum number of significant figures through to final answer then round to one decimal place. Do not include units.Of Aspirin, ethanol, acetaminophen, ethyl acetate, hexane, and water which could go undetected during HPLC analysis, but could result in a lower than expected melting point, why are they not detected by HPLC analysis?