Explain the mechanism leading to the given reactions in the inoculated biochemical media.  1. Blackening of the medium in Triple Sugar Iron Test slant

Q: Briefly define culture medium.

A: Microbiology is the branch of science which deals with the study of microorganisms like, bacteria,…

Q: The number of bacteria in the samples need to be quantified. The laboratory uses a specific protocol…

A: Serial dilution is a process in which we dilute the sample and do passage, transfer, which is…

Q: For each of the following aspects, compare and contrast the EnteroPluri-Test System to the API® 20E…

A: EnteroPluri-Test is a 12-sector system that consists of special culture media. which allows…

Q: js1 /7 w Liquid media : Presence of 0.2-0.5% agar. e.g. Motility test. А. are correct В. are false…

A: Liquid media is usually preferred for growing large microorganisms along with some agar.

Q: Question 5 Match the media to its functional classification TS broth or TSA [ Choose ] [Choose ]…

A: A bacterial medium is a medium which supports the growth of bacteria by providing the necessary…

Q: Explain why the following steps are essential during subculturing:a. Flaming the inoculating…

A: A microbial culture made by transferring the cells from the previous/old culture medium into a fresh…

Q: Samples subjected to a test were given. Determine the reagent/material in the test.  Methyl…

A: Different chemical tests use different reagents and apparatus depending on their working principle.

Q: organisms are being differentiated in the butyrate disc test.

A: The Butyrate test is a rapid detection test for the butyrate esterase enzyme.

Q: In product testing, microbial growth is found.| Interpretation:  Rationale:

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: 4. Complete the table for Watson-Schwartz Test Results for porphobilinogen and urobilinogen…

A: Watson Schwartz test: This test detects the intermittent porphyria by identifying the presence of…

Q: Match the test with a positive result  a. DNA test  b. Paper chromatography test c. Celllar…

A: Genetic material is nothing but the sequence of nucleic acids which is called as DNA. It contains…

Q: 1. One mL of sludge was added to a 99 mL dilution blank. Three 1/10 dilutions were then made. From…

A: CFU/ml represents the number of colony-forming units in the sample. It gives us the estimation of…

Q: 1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating…

A: Agar is formed by two components, agarose, and agaropectin molecules. Agar is used as culture media…

Q: Explain the rationale behind Macrohematocrit test (Wintrobe method) and Microhematocrit test (Clay…

A: Hematocrit test is used to check the proportion of red blood corposcles (RBC) present. If the RBCs…

Q: Koch's postulates include all of the following EXCEPT O 1) Isolate the organism in pure culture. The…

A: Koch's postulates are a series of findings and experimental criteria suggested in the late 1800s by…

Q: In the Kirby-Bauer test, which of the following must be consistent? a. concentration of bacteria…

A: Kirby-Bauer test: It is an antibiotic test also known as KB testing. It is used to test which…

Q: Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions…

A: Note- we are supposed to answer three subpart of a question according to our guidelines. Please…

Q: neration time of the species is 1 hour, and the microbes are in the exponential (log) hase of…

A: Microorganism that experience exponential growth its number increases according to this equation…

Q: Can culture media be sterilized in the hot air sterilizer? Why or why not? (Answer in not more than…

A: Sterilization is defined as a process by which an agent is used to destroy or remove the…

Q: Provide a brief description and state the function of the following tools and equipment used in the…

A: A laboratory is a facility that provides controlled conditions in which scientific or technological…

Q: Citrate test What is the color of the medium before inoculation? Green or blue

A: Biochemical method of bacterial identification is a classical method that is used for the…

Q: Using the Table above as a guide,   indicating what media could be used to differentiate the…

A: Introduction :  The bacterium Staphylococcus epidermidis is gram-positive. It is a natural component…

Q: What are the two ways that nitrate can be reduced by the bacteria. Explain briefly.  2. What…

A: Denitrification - It is the process by which nitrate (NO3-) is reduced to produce nitrogen (N2) gas.…

Q: Give the importance and purpose of the Cetrimide Test. 2. being performed - Dry Filter Paper…

A: Disclaimer: Since you have asked multipart question I will answer  3 question. Introduction The…

Q: List the different reagent strip tests with their principle 2. Enumerate 5 clinical significance…

A: DIFFERENT REAGENT STRIP TEST:- 1.Urobilinogen 2.Bilirubin 3.Ketone 4.Blood 5.Protein 6.Nitrite…

Q: Citrate test Explain what is the color of the medium after incubation? Green or blue

A: To correctly identify a microbial species, we make use of various biochemical assays that have…

Q: The catalase test is used to primarily differentiate Staphylococi from _____________.     A.…

A: The enzyme, catalase, is produced in the bacteria that respire using oxygen. The main role of the…

Q: Procedure Take Add 50 ul of Top up to 50ml. fresh LB- ampicillin Mix Smi ml to the overnight culture…

A: Two culture mediums are prepared using E.coli and LB borth. Both the mediums are prepared in…

Q: 1. One gram of sludge was added to a 99 mL dilution blank. Three 1/10 dilutions were then made. From…

A: Colony-forming units per milliliter, or CFU/ml, are measured in the sample. It provides us with an…

Q: Test Description Terminal Terminal Medium, Incubation Incubation Results temperature time (°C)…

A: A difference in the physiology of bacteria from one to another is the necessity to utilize…

Q: An inoculating loop must be flamed to uniform orange-hot from base to tip   A. after culture is…

A: An inoculation loop is a tool used by microbiologists to transfer a small culture of microorganisms.…

Q: Which of the results shown in the figure (Test A, B, or C) used Biuret Reagent? TEST A TEST B TEST C…

A: Peptides are defined as a short string of amino acids where it contains only 2 to 50 amino acid…

Q: 5. Provided with the following data, compute the corresponding CFU/ml of the original culture.…

A: Answer :: Introduction The plate is considered to be countable if it has a colony count between 30…

Q: From figure  shows triplesugar iron agar. What biochemical characteristics does this…

A: “Triple sugar iron test” is one of the “biochemical tests” used to test the ability of microbe in…

Q: write a detailed description of each of the following tests/media: a. TSI Agar Slant b. SIM Medium…

A: The biochemical and microbial tests are performed to analyze the capability and function of the…

Q: Observations and Results Average Count per ml of Sample (CFU/ml) Bacterial Dilution Factor ml of…

A: Introduction The Stepwise Dilution Of A Substance In Solution Is Referred To As Serial Dilution.…

Q: Explain the concept of and apply the aseptic technique

A: Bacteria, viruses, and fungi are the microorganism that is present everywhere. Some of them cause…

Q: Give the use and advantages of the following cultures for the examination of colony characteristics:…

A: Culture media: a. Culture media provides an environment that supplies all essential nutrition for…

Q: How can I explain the results of my findings in lamen terms?

A: These test are the perfomed to identify positive and negative result for glucose fermentation.

Q: discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple…

A: A mixed culture is one that contains more than one type of organism growing in a sterile medium. The…

Q: Benedicts Test: Reagent’s Composition   Observations: Test Sample Observations 0.1M glucose   0.04…

A: Glucose is a monosaccharide that contains six atoms of carbon and an aldehyde group, hence, it is an…

Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. You then…

A: A serial dilution is any dilution in which the concentration is decreased by the same factor in each…

Q: Examine the given sets of table below. Which disinfectant is the MOST recommended? Support and…

A: given here are set of disinfectants with their phenol coefficient and growth of bacteria after…

Q: Use the dilution scheme to answer. The concentration of bacteria in the original sample is CFU/ml.…

A: The Entire Viable Count refers to the total number of colonies (TVC). The cfu/ml (colony forming…

Q: 5. Provided with the following data, compute the corresponding CFU/ml of the original culture.…

A: Introduction For CFU count the average no. of colonies chosen is 30-300. Above this range, the…

Q: Kligler’s iron agar and SIM are multiple test media.. Both media are stabbed but for different…

A: The liquid or solid substratum on which, the organ or cell explants can be grown. The medium…