What does the Kirby-Bauer (disk diffusion) test assess? O The ability of a microorganism to produce an antibiotic. O The ability of a microorganism to be susceptible to antibiotics. The ability of a microorganism to swim away from an antibiotic. O The ability of an organism to mutate in the presence of an antibiotic. O The ability of a microorganism to synthesize an antibiotic,

What does the Kirby-Bauer (disk diffusion) test assess? O The ability of a microorganism to produce an antibiotic. O The ability of a microorganism to be susceptible to antibiotics. The ability of a microorganism to swim away from an antibiotic. O The ability of an organism to mutate in the presence of an antibiotic. O The ability of a microorganism to synthesize an antibiotic,

Name: What does the Kirby-Bauer (disk diffusion) test assess?O The ability
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Description: What does the Kirby-Bauer (disk diffusion) test assess?O The ability of a microorganism to produce an antibiotic.O The ability of a microorganism to be susceptible to antibiotics.O The ability of a microorganism to swim away from an antibiotic.O The

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter22: Prokaryotes: Bacteria And Archaea

Section: Chapter Questions

Problem 23RQ: Which of these statements is true? An antibiotic is any substance produced by a organism that is...

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During the Ames test, his− bacteria are exposed to a mixture of chemical compounds extracted from SurbWay Sandwich. If the his− bacteria revert to his+, then what can we say about the chemical?
Bacterial species that are harmful, as well as others that are beneficial, are found living in the human body. To slow the rate of developing antibiotic resistance in bacteria, physicians are being encouraged to use "narrow-spectrum" antibiotics- those that target only a few bacterial types. How can the information learned by antibiotic sensitivity testing be used by physicians who must choose antibiotics that inhibit the growth of bacteria causing disease but that do not interfere with beneficial bacteria?
The Kirby-Bauer test examines the effectiveness of antibiotics and disinfectants or antiseptics on separately plated bacteria. Which of the following Zones of Inhibition (ZOI) measurements on a plate of Klebsiella pneumoniae shows the strongest inhibition of the bacterium's growth? a) Penicillin, 7 mm ZOI b) Actinomycin, 9 mm ZOI c) Tetracycline, 26 mm ZOI d) Chloramphenicol 2 mm ZOI e) Streptomycin, 23 mm ZOI
Can the results of an MIC test be used to determine whether an antibiotic is bactericidal or bacteriostatic? If not, set up an experimental procedure to determine whether the effect is bactericidal or bacteriostatic.
If a bacterial species is not susceptible to an antibacterial drug at the concentration present in a particular disk, does that necessarily mean the species is completely resistant to the drug? Explain your answer. Did you notice any colonies (isolated mounds of cells) growing within any of the zones of inhibition? If so, which plate(s) and which drug(s)? What would cause growth of colonies within a zone of inhibition? please answer both questions.
. Which agar plate(s) would tell you if bacteria have been transformed? Circle the right answer(s) and explain why. ((a) LB / Ampicillin +pGLO (b) LB / Ampicillin / Arabinose +pGLO (c) LB / Ampicillin -pGLO (d) LB -pGLO (e) All the above And Why?
PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in.
PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in. • Leave the petri dishes uncovered while the bacteria grow. , cover the petri dishes while the bacteria grow. Treat the bacterial culture with a disinfectant before spreading on the agar plate Wear personal protective equipment (gloves, mask, lab coat) while performing the procedure Wear…
Petri dish. Explain in your own words how bacteria are grown to form the sample for the antibiotic resistance test?
Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.
Compare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects on the optical density (OD) and viable count of a bacterial culture, respectively?
A bacteriophage stock with a concentration of 1.45 x 1014 PFU/ml is prepared for an experiment. After dilution, 0.5 ml of the stock is plated and 100 plaques are found. What is the dilution factor used in this process? Answer in scientific notion with 3 significant digits.