What is G-banding? O A) Most commonly used banding method B) Uses giemsa dye O C) Produces alternating dark and light banding O D) All of the above

Q: What is replica plating?

A:

Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain

A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…

Q: Why is the order of separation based on size opposite for gel filtration and gel electrophoresis,…

A: Gel filtration and gel electrophoresis are biochemical techniques used for the separation of…

Q: What is ninhydrin used to detect? How does it work?

A: Proteins are involved in several important body functions and are very much essential for all life…

Q: When viewing the capsule stain, why do the capsules appear colorless? Explain

A: According to guidelines we have to answer the first question only. so please kindly post the…

Q: Why safranin is used as the sample dye?

A: Safranin is utilized as a counterstain in some staining conventions, shading cell cores red. This is…

Q: What is covalently attached to the resin beads for us to purify GST-DHFR-His by IMAC? Group of…

A: Answer given below..

Q: Why might Gamma irradiation be considered better than using UV radiation?

A: It is required to provide reason that Gamma irradiation is considered better than using UV radiation…

Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…

A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…

Q: Why should there be 3 banding techniques (G, Q, and R)? Aside from their differences in appearance…

A: Banding techniques are utilized to understand the staining effects in the chromosomes so that the…

Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?

A: bacteria like  Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…

Q: Why were radioactive sulfur and phosphorous used to label bacteriophage in Hershey and Chase's…

A: Alfred Hershey and Martha chase did so in order to label the protein coat of virus with radioactive…

Q: What is the principle of Giemsa staining and  components of Giemsa stain?

A: The cells are the basic structural and fundamental unit of the living system. It consists of…

Q: Write the procedure for celloidin embedding.

A: Hi! Thanks for your question. But as you have posted multiple questions, I am answering the first…

Q: What is known as montreal protocol?

A: Montreal protocol is an international initiative for ozone depletion.

Q: Can two-dimensional gel electrophoresis be used as a purificationtechnique? Explain.

A: Gel electrophoresis is a laboratory technique, which is used to separate mixtures of DNA or RNA…

Q: hat Is Polychrome Methylene Blue?

A: Methylene blue is a popular dye that allows us to glimpse microscopic life in vibrant colors. Before…

Q: What do you mean by fluorescent-labeled probes?

A: Fluorescent marking is the interaction of covalently binding fluorescent colors to biomolecules,…

Q: What are the 5 steps of gel electrophoresis?

A: Gel electrophoresis is a method for separating DNA fragments (or other macromolecules like RNA and…

Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?

A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…

Q: Can colorless samples be analyzed using a UV-Vis spectrophotometer? Yes or No or Maybe?

A: UV-Vis Spectroscopy  It is defined as a quantitative technique that is used to identify the…

Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…

A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…

Q: Is K2EDTA same as Spray-coated K2EDTA?

A: K2EDTA stands for dipotassium ethylenediaminetetraacetic acid and is a strong anticoagulant used…

Q: Why

A: Introduction :- Electrophoresis is a technique commonly used used to separate charged molecules,…

Q: In extraction of albumin in egg white using 100-ml water, what does albumin look like? Also, what do…

A: In extraction of albumin in egg white and placed in water some reactions are occur..

Q: t is the principle of gel electrophoresis?

A: The purity of isolated nucleic acids is determined using electrophoresis.

Q: What is the principle of the gel electrophoresis separation technique?

A: Biological techniques are procedures or strategies for studying biological things. Biological…

Q: About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this…

A: Gram staining technique is a differential test based on the composition of bacteria cell wall. It…

Q: What are the benefits and disadvantages of IMAC for purifying proteins?

A: The purification of the proteins is performed by the use of different chromatographic procedures…

Q: What are the different applications of Gel Electrophoresis?

A: The variation in size and charge of distinct molecules in a sample is exploited by the gel…

Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: What is Ultraviolet (UV) light ?

A: The different kinds of EM radiation that are part of the electromagnetic spectrum, namely: Gamma…

Q: What is a crystal violet stain and its purpose?

A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…

Q: What are decapping enzymes ?

A: Introduction: Decapping is the process that includes the hydrolysis of the 5` cap structure from the…

Q: What types of compounds make up the gels used in electrophoresis?

A: The types of macromolecules usually separated on Agarose gel are : Nucleic acids-mainly DNA…

Q: in paper chromatography what is the basis of pigments separate?

A: Paper chromatography is a technique in which different types of pigments can be separated from each…

Q: what are two differences between agarose gel electrophoresis and SDS PAGE?

A: Agarose gel electrophoresis and the SDS page technique are used to separate the molecules. The…

Q: define the term multicolor banding,

A: Chromosomes are long thread-like structures that carry coded genetic information in the form of DNA.…

Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?

A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…

Q: The DNA fragments separated on an agarose gel can be visualised after staining with what?

A: Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis…

Q: What is gel electrophoresis?

A: All living systems are made up of chemical molecules, which interacts with each other to perform…

Q: how does polyacrylamide gel electrophoresis with SDS separate proteins ?

A: Answer: SDS = Sodium Dodecyl Sulfate is a detergent used in the polyacrylamide gel electrophoresis…

Q: What type of chromophore is associated with a negative stain?

A: Bacteria are unicellular prokaryotic organisms that maintain a definite shape since they possess the…

Q: What is the basic purpose of the paper chromatography?

A: Chromatography is an important analytical method used to separate a mixture of chemical substances…

Q: Name two fixatives used in staining?

A: Histology is the study of ultrastructure of tissues using thin sectioning techniques like microtomy,…

Q: .What methods are used to visualize radioactively labeled nucleic acids?

A: Electrophoresis is a laboratory method that uses an electric field to separate molecules. The charge…

Q: Write the procedure for celloidin  embedding

A: Answer: Celloidin = It is basically the semisolid solution of pyroxylin in ether or alcohol, which…