Q: What is the purpose of the alcohol (OH) in the gram staining process?
A: Usually, microbes are colorless, so it’s very difficult to see them even after magnifying under…
Q: Explain the reasons you would use a negative stain?
A: In a negative stain the slide is actually stained. This is because the negatively charged stain…
Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain
A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…
Q: What is the usual stain used for staining a sputum smear?
A: Essentially, sputum comprises of a combination of saliva and mucus. additionally named as phlegm, It…
Q: What are the sources of error in performing Gram staining?
A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…
Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin
A: The living cells can be seen under a microscope. The cells is not visible properly as it is…
Q: What is gram staining and its purpose? Why is it only applicable in bacteria?
A: Introduction: Differential staining methods like Gram staining, acid-fast staining, endospore…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes?
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: Why is the Gram stain considered a differential stain?
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: For a stain to be considered differential, what must it do?
A: Differential stain uses more than two stains.
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: Which step is the most crucial or most likely to cause poor results in the Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods
A: Ziehl neelsen staining principle The presence of mycolic acids in the cell walls of acid-fast…
Q: What is a differential stain? why is it used?
A: Differential stain is a type of stain use to identify organisms.
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.
A: Staining is a strategy used to improve contrast in examples, for the most part at the minuscule…
Q: What are the most commonly used methods in staining? why?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a tiny scale.…
Q: How are negative stains prepared?Name two applications for which this type of stain especially…
A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…
Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…
Q: What are the functions of a primary stain and a counterstain?
A: The main stain and the counterstain must be of different colours so that the two forms of bacterial…
Q: What are the Types of Differential Stains? explain with an example.
A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?
A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: Why is it necessary to perform a negative stain to visualize capsules?
A: Introduction: Negative staining: This uses an acidic stain. The results yield a clear cell with a…
Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: What is an example of a negative stain?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: What's the theory and conclusion of a acid fast stain?
A: It was Ziehl who originally devised differential staining procedures, that were later refined by…
Q: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic…
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: The decolorizer used in the Gram Stain, and the decolorizer used in the Acid Fast Stain, are the…
A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the…
Q: What is the difference between Giemsa and Wright stain?
A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…
Q: What is the mordant in the spore stain?
A: A mordant is any substance, which is used as a fixative to bind the dye on the substance.
Q: list the stains and decolorizing agent used in acid fast staining method.
A: Certain bacteria have a physical property known as acid fastness. Property:- Resistance to…
Q: What is a crystal violet stain and its purpose?
A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…
Q: Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid…
A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…
Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…
A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…
Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…
A: Answer First option Non-acid-fast microbes appear blue in a completed acid-fast stain.
Q: Why is it incorrect to say that the GFP is a “staining” method?
A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…
Q: Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each…
A: Bacteria are ubiquitous microscopic , one celled organism which possess variety of shape ,…
Q: k there are 2 staining reagents applied in differential s
A: Differential Staining- It is a staining process which uses more than one staining reagent. it is…
Q: What is the type of gram stain?
A: Gram stain or Gram staining, also called Gram's method, is a method of staining used to classify…
Q: What color wouod gram negative cells appear if the decolorizing step was left out of staining…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: What type of chromophore is associated with a negative stain?
A: Bacteria are unicellular prokaryotic organisms that maintain a definite shape since they possess the…
Q: What are the principle and basic concepts of GRAM STAIN?
A: Gram staining is among the most important microbiology staining procedures. It was first invented in…
Q: How can the thickness of the smear affect the staining procedure?
A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…
Q: What staining technique is used here? What is the primary stain, decolorizing agent, and counter…
A: Gram Staining is the common, important, and most used differential staining techniques in…
Q: What is the difference between simple and differential stains? Give an example of a differential…
A: Bacteria are microorganisms that are colorless for most of the part and in order to study their…
Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…
A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…
What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain, what is the mordant?
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- What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?When it comes to simple stains, why is time so important? And what is the purpose of rinsing the slide in water after each Gram staining procedure?What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.
