In extraction of albumin in egg white using 100-ml water, what does albumin look like? Also, what do you call the bubbles produced at the top of the solution?
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In extraction of albumin in egg white using 100-ml water, what does albumin look like? Also, what do you call the bubbles produced at the top of the solution?
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- If you have an absorbance of 2.0 mL of stock dye, what kind of a dilution is needed to get it to 1.0? How do you go about this dilution and how do you calculate?in paper chromatography what is the basis of pigments separate?What molecules are present in regions of gel corresponding to A? What happens upon heatshock? What does Band B represent?
- How long does it take fir alcohol to completely denature the albumin proteinsUsing absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?What will be happen if you mix; 1ml of 5% solution of albumin and 5 mL of 95% ethanol 1ml of 5% solution of gelatin and 5 mL of 95% ethanol 1ml of 5% solution of peptone and 5 mL of 95% ethanol Mix and observe the formation of precipitate.
- In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?What makes transparent softening lotion different from suspension softening lotion? Is it because of capric triglyceride makes it suspension?Why do detergent powders removes stain? Does a type of detergent powders differs in terms of rate in removing stains? Why or why not? Explain. Why do you think is the reaspn why some stains are easy to remove while the others are tough? Is it something to do with the detergent powder used or to the type of stain? Expain and justify.
- Why is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.For a TLC experiment, what is the purpose of the pentane extraction of spinach juice? Why wouldn’t we want to spot an aqueous solution onto the silica gel?How is xylol used to clean the lens of a microscope? What may happenif xylol is constantly used for this purpose?
