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What is the fate of the radioactive label when each of the following pairs of compounds is added to a cell extract containing the enzymes and cofactors of the glycolytic pathway, the citric acid cycle, and the pyruvate dehydrogenase complex? The 14C label is noted with an *. + unlabelled oxaloacetate a) H3C- pyruvate b) .CO + unlabelled pyruvate H2 охaloacetate Guidelines • Show enough structures along the way so your logic can be followed. You do not need to show every structure along the way. • Go around the citric acid cycle until all of the label has come off as CO2 (or until you can see a pattern develop - you can then just state the pattern in words). If you need to go around the citric acid cycle more than once (it won’t for this problem!), assume that the acetyl CoA that enters on all subsequent turns is not labeled.

What is the fate of the radioactive label when each of the following pairs of compounds is added to a cell extract containing the enzymes and cofactors of the glycolytic pathway, the citric acid cycle, and the pyruvate dehydrogenase complex? The 14C label is noted with an *. + unlabelled oxaloacetate a) H3C- pyruvate b) .CO + unlabelled pyruvate H2 охaloacetate Guidelines • Show enough structures along the way so your logic can be followed. You do not need to show every structure along the way. • Go around the citric acid cycle until all of the label has come off as CO2 (or until you can see a pattern develop - you can then just state the pattern in words). If you need to go around the citric acid cycle more than once (it won’t for this problem!), assume that the acetyl CoA that enters on all subsequent turns is not labeled.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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What is the fate of the radioactive label when each of the following pairs of compounds is added to a cell extract containing the enzymes and cofactors of the glycolytic pathway, the citric acid cycle, and the pyruvate dehydrogenase complex? The 14C label is noted with an *. || + unlabelled oxaloacetate a) H3C- * pyruvate b) unlabelled pyruvate + H2 охaloacetate Guidelines Show enough structures along the way so your logic can be followed. You do not need to show every structure along the way. • Go around the citric acid cycle until all of the label has come off as CO2 (or until you can see a pattern develop - you can then just state the pattern in words). If you need to go around the citric acid cycle more than once (it won't for this problem!), assume that the acetyl CoA that enters on all subsequent turns is not labeled.
Transcribed Image Text:What is the fate of the radioactive label when each of the following pairs of compounds is added to a cell extract containing the enzymes and cofactors of the glycolytic pathway, the citric acid cycle, and the pyruvate dehydrogenase complex? The 14C label is noted with an *. || + unlabelled oxaloacetate a) H3C- * pyruvate b) unlabelled pyruvate + H2 охaloacetate Guidelines Show enough structures along the way so your logic can be followed. You do not need to show every structure along the way. • Go around the citric acid cycle until all of the label has come off as CO2 (or until you can see a pattern develop - you can then just state the pattern in words). If you need to go around the citric acid cycle more than once (it won't for this problem!), assume that the acetyl CoA that enters on all subsequent turns is not labeled.
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