Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
What is the impact of new km value 0.1mM compared to previous km value 0.05mM on affinity of the enzyme for substrate?

What is the impact of new km value 0.1mM compared to previous km value 0.05mM on affinity of the enzyme for substrate?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
What is the impact of new km value 0.1mM compared to previous km value 0.05mM on affinity of the enzyme for substrate?
Michaelis Menten Plot.als (Compatibility Model - Microso Home Insert Page Layout Formulas Data Review Acrobat * Cut a Copy Format Painter Calibri 11 AA Wrap Text General BIU - A FI ER Merge & Center S% A Conditional Form Formatting- as Tabi Cipboard Fent Agnment C20 K L M C D Calc. Vo A F. G H 1 (Ethanol) Vo delta 2 0.007 0.06 0.020242 0.001581 035 3 0.015 0.11 0.040357 0.00485 4 0.031 0.16 0.073219 0.007531 03 0.068 0.21 0.125236 0.007185 025 6 0.1 0.23 0.154704 0.00567 0.2 0.28 0.206272 0.005436 0.2 0.3 0.29 0.232056 0.003358 0.28 0.247526 0.001055 Calc. Vo 0.4 0.15 10 0.036664 01 11 Km 0.1 12 Vmax 0.309407 0.05 13 14 16 0.1 02 0.3 0.4 0.5 17 18 19 Fig. 9. Michaelis-Menten Plot using a value of 0.1 mM for Km. Notice: 1) that a new set of calculated values in column C has been generated and 2) that by using these new data a new best fit Michaelis-Menten plot (red curve) has been generated. Fig. 9 shows that the rates for the new enzyme-catalyzed reaction (red curve) were even lower than those obtained with a value of Km of 0.05 mM (red curve in Fig. 8).
Transcribed Image Text:Michaelis Menten Plot.als (Compatibility Model - Microso Home Insert Page Layout Formulas Data Review Acrobat * Cut a Copy Format Painter Calibri 11 AA Wrap Text General BIU - A FI ER Merge & Center S% A Conditional Form Formatting- as Tabi Cipboard Fent Agnment C20 K L M C D Calc. Vo A F. G H 1 (Ethanol) Vo delta 2 0.007 0.06 0.020242 0.001581 035 3 0.015 0.11 0.040357 0.00485 4 0.031 0.16 0.073219 0.007531 03 0.068 0.21 0.125236 0.007185 025 6 0.1 0.23 0.154704 0.00567 0.2 0.28 0.206272 0.005436 0.2 0.3 0.29 0.232056 0.003358 0.28 0.247526 0.001055 Calc. Vo 0.4 0.15 10 0.036664 01 11 Km 0.1 12 Vmax 0.309407 0.05 13 14 16 0.1 02 0.3 0.4 0.5 17 18 19 Fig. 9. Michaelis-Menten Plot using a value of 0.1 mM for Km. Notice: 1) that a new set of calculated values in column C has been generated and 2) that by using these new data a new best fit Michaelis-Menten plot (red curve) has been generated. Fig. 9 shows that the rates for the new enzyme-catalyzed reaction (red curve) were even lower than those obtained with a value of Km of 0.05 mM (red curve in Fig. 8).
Your worksheet and graph should look similar to the figure below. Michaelis Menten Plot.ds [Compatibility Mode] - Microsot Home Insert Page Layout Formulas Data Review View Add-Ins Acrobat * Cut Wrap Text General Calibri Copy Conditional Forma Paste BIU- E Merge & Center Format Painter Formatting as Tabl Clipboard Font Alignment Number C16 B G H K M 1 [Ethanol] Vo Calc. Vo delta^2 2. 0.007 0.06 0.037997 0.000484 0.35 0.015 0.11 0.071402 0.00149 4 0.031 0.16 0.118415 0.001729 0.3 5 0.068 0.21 0.178303 0.001005 0.25 0.1 0.23 0.206272 0.000563 0.2 0.28 0.247526 0.001055 0.2 0.3 0.29 0.265206 0.000615 • Vo 0.4 0.28 0.275029 2.47E-05 0.15 Calc. Vo 10 0.006965 0.1 11 Km 0.05 12 Vmax 0.309407 0.05 13 14 16 0.1 0.2 0.3 0.4 0.5 17 18 Fig. 8. Michaelis-Menten Plot using a value of 0.05 mM for Km. lotice: 1) that a new set of calculated values in column C has been generated anc
Transcribed Image Text:Your worksheet and graph should look similar to the figure below. Michaelis Menten Plot.ds [Compatibility Mode] - Microsot Home Insert Page Layout Formulas Data Review View Add-Ins Acrobat * Cut Wrap Text General Calibri Copy Conditional Forma Paste BIU- E Merge & Center Format Painter Formatting as Tabl Clipboard Font Alignment Number C16 B G H K M 1 [Ethanol] Vo Calc. Vo delta^2 2. 0.007 0.06 0.037997 0.000484 0.35 0.015 0.11 0.071402 0.00149 4 0.031 0.16 0.118415 0.001729 0.3 5 0.068 0.21 0.178303 0.001005 0.25 0.1 0.23 0.206272 0.000563 0.2 0.28 0.247526 0.001055 0.2 0.3 0.29 0.265206 0.000615 • Vo 0.4 0.28 0.275029 2.47E-05 0.15 Calc. Vo 10 0.006965 0.1 11 Km 0.05 12 Vmax 0.309407 0.05 13 14 16 0.1 0.2 0.3 0.4 0.5 17 18 Fig. 8. Michaelis-Menten Plot using a value of 0.05 mM for Km. lotice: 1) that a new set of calculated values in column C has been generated anc
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS