Four competitive inhibitors of an enzyme were found to exhibit the following K1 values. Which is the worst inhibitor? K1 = 7 x 10-5 M KI = 3 x 10-6 M KI = 1 x 10-4 M KI = 6 x 10-8 M
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A: Hello. Since your question has multiple sub-parts, we will solve first three sub-parts for you. If…
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- Question 1: ATP synthases contains 3 copies of each of the large subunits (called α and β in the E. coli enzyme). The number of c subunits can vary among different ATP synthases, ranging from 8 to 17. Part a: Briefly explain how this variation in the number of c subunits would affect the energetics of ATP synthesis. For a fixed value of the protonmotive force, how much energy can the gradient provide to drive one revolution of the ATP synthase, in a synthase with 8 c- subunits versus a synthase with 17 c-subunits?Question:- 33) Glucose-6-phosphate has different pathways that it can enter in the liver during the fed state. All of the following are possible except OA.) it is oxidized to acetyl CoA for fatty acid biosynthesis O B). it can enter the pathway for glycogen biosynthesis O c.) it is a substrate for glucose-6-phosphatase OD. )it is oxidized in glycolysis to provide energy for the bodyQuestion:- 2) oxaloacetate (OAA) occurs as an important intermediate in 2 metabolic processes a) indicate these reaction steps where OAA occurs b) indicate structure for OAA 3) how many reduced equivalents (as electron carrier) are obtained after an oxidation of C16H12O2? describe in detail the structure of these steps.
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- QUESTION 2An isocitrate dehydrogenase assay was performed on the enzyme sample and found to give an absorbance change at 340nm of 0.5 absorbance units perminute. Given that the molar absorption coefficient (E) is 6220 M-1 cm-1 and the pathlength is 1cm, what is the rate of the enzyme catalysed reaction in umol perminute per mL?Question:- 1.) If 2 molecules of glucose enters glycolysis, a total of how many carbon dioxide molecules are released after Krebs Cycle including those released during pyruvate processing?Question 9 - Under anaerobic conditions in the muscle, lactate dehydrogenase catalyzes the reaction converting pyruvate to lactate. What is the metabolic reason for this fermentation reaction? It is necessary to reduce pyruvate before it can be converted to acetyl-CoA and enter the citric acid cycle in muscle. NAD* must be replenished for glycolysis to continue. The reaction creates NADH which can produce additional energy in the electron transport chain. O Lactate is more oxidized than pyruvate.
- Question 1: The standard reduction potential for the the cytochrome c Fe3+/Fe2+ redox couple and for the dioxygen/water redox couple. Part a: Use these values, and the relevant equation, to compute the standard free energy change for the transfer of 4 electrons from cytochrome c to dioxygen. Part b: Next, compute the free energy needed to pump four protons across the mitochondrial membrane, using the relevant equation and values of -.14V for Δψ and 1.4 for ΔpH. Part c: If energy conservation by cytochrome c oxidase involved only the four pumped protons (and standard conditions applied), what would be the efficiency of energy conservation at this step? (what is the ratio [energy conserved in the gradient] / [redox energy used] ?)Question: A. To explore the consequences of coupling ATP hydrolysis under physiological conditions to a thermodynamically unfavorable biochemical reaction, consider the hypothetical transformation X⟶Y, for which Δ?′°=20.0 kJ/mol. What is the ratio of [Y]/[X][Y]/[X] at equilibrium? B. Suppose XX and YY participate in a sequence of reactions during which ATP is hydrolyzed to ADP and Pi. The overall reaction is X+ATP+H2O⟶Y+ADP+Pi Calculate [Y]/[X] for this reaction at equilibrium. Assume that the temperature is 25.0 °C and the equilibrium concentrations of ATP, ADP, and Pi are 1.00 M each. C. We know that [ATP], [ADP], and [Pi] are not 1.00 M under physiological conditions. Calculate [Y]/[X] for the ATP‑coupled reaction when the values of [ATP], [ADP], and [Pi] are those found in rat myocytes. Metabolite Concentration in rat myocytes (M) ATP 8.05x10-3 ADP 0.93x10-3 Pi 8.05x10-3Question 56 You (a healthy individual) are swimming underwater when you can no longer resist the urge to breathe. This overwhelming urg probably due to O the autorhymthic cells in your diaphragm contracting O the decrease in O 2 available to the cells of the body. O the increase in plasma H *. O the increase in pH has made your blood dangerously alkaline. 1.67