What is the isoelectric point, pl, for the pentapeptide, MGHFD 4.8 3.1 Show work O 7.8 2.3 O 9.5

The discovery of green fluorescent protein (GFP) in the bioluminescent jellyfish Aequorea victoria earned the Nobel Prize in chemistry in 2008. Briefly describe an important application of GFP.

How do we know that IBr is polar and CO2 is non polar?

Describe two examples of protein folding patterns and identify what level of protein structure they belong to. What other reagents might you use besides ferric chloride to test affinity for metal ions? How many grams of the protein are in one 200 g glass of milk?

Suppose a chloride ion and a sodium ion are separated by a center–centerdistance of 5 Å. Is the interaction energy (the energy required to pull theminfinitely far apart) predicted to be larger if the medium between them iswater, or if it is n-pentane? (as shown)If Ca2+, Na+, and F- each have ionic radii ~1.16. Which ionic bond is stronger: Ca-F or Na-F?If Ca2+ is often bound on the surface of a protein by carboxylic acid functional groups. If the pKa of a particular -COOH group is 4.2, wouldyou predict Ca2+ to be most tightly bound at pH 8, pH 4.2, or pH 3? Explain your answer.

Suppose two phosphate groups in DNA (each with a charge of 21) are separated by 12 Å. What is the energy of the ionic interaction between these two phos- phates assuming a dielectric constant of 80? Repeat the calculation assuming a dielectric constant of 2.

Consider the formation of a dimeric protein 2P  ->P2   At 25.0 °C , the enthalpy of the reaction is 17 kj/mol and the entropy of the reaction is 65 j/kmol. Is the dimerization favored at this temperature ? Thermodynamically speaking, explain/support your answer. Show work.

Suppose a chloride ion and a sodium ion are separated by a center–center distance of 5 Å. Is the interaction energy (the energy required to pull them infinitely far apart) predicted to be larger if the medium between them is water, or if it is n-pentane?  If Ca 2+, Na+, and F - each have ionic radii ∙1.16. Which ionic bond is stronger: Ca-F or Na-F? If Ca 2+ is often bound on the surface of a protein by carboxylic acid functional groups. If the pKa of a particular ¬COOH group is 4.2, would you predict Ca 2+ to be most tightly bound at pH 8, pH 4.2, or pH 3? Explain your answer.

NH3 has a dipole moment of 1.47 D. Consider the interaction energy between Na+ and NH3 in an aqueous solution with a separation distance of 0.2 nm. (εH2O = 80.10) Calculate the interaction potential for each of the ion-dipole orientations shown below and determine the orientation with the lowest potential energy.

Define the term SDS page.