What is the name of the method you used to isolate protein from bacterial sample
Q: How do you make liquid bacteria culture?
A: It is important for growing more numbers of bacteria to isolate enough plasmid DNA for experimental…
Q: How do you prepare 50 ml of 20 mg/ml proteinase K from 50 mg/ml stock solution
A: Proteinase K is a serine proteinase enzyme used to digest proteins. It is produced from a…
Q: Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…
Q: What is the purpose of the bacterial capsule? Answer in not more than 3 sentences.
A: Prokaryotic calls contain addition protective layer outside of the bacterial cell wall. It is…
Q: tate the apparatus used view small samples
A: The world is full of organisms. Organisms have developed from minute single celled structures to…
Q: Write introduction about Identification of bacteria &Bacterial Staining?
A: Bacteria are unicellular prokaryotic microorganisms (nucleus is not enclosed in a nuclear membrane)…
Q: Why is oil immersion necessary to see bacteria in the laboratory?
A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…
Q: What tools are used to transfer bacteria
A: Ans - An inoculation loop is used to transfer bacteria for microbiological culture. Petri dish.…
Q: What is another test you could use to determine the amount of protein?
A: The nutrients can be classified into three groups, water, micronutrients, and macronutrients. Water…
Q: Examples of bacteria observe in the microscope using Gram stain, Ziehl-Neelsen, and Kinyoun Method.
A: Ziehl–Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen…
Q: List 6 common bacterial shapes.
A: Bacteria is a type of microorganism and consists of a single, prokaryotic cell. They lack a…
Q: You are using bacterium E.coli to synthesize a protein from recombinant DNA. The protein remains in…
A: Escherichia coli is one among the organisms of selection for producing recombinant proteins. It's…
Q: What color is a GRAM NEGATIVE bacteria? purple pink
A: Microscopic, single celled organisms which are found in every environment in millions are known as…
Q: Discuss why you should incubate bacterial plate upside down with the lid at the bottom:
A: Management of the culture plates is important in the microbiology laboratory. It is studied under…
Q: Which one of the following is NOT considered a biohazard? old bacterial cultures slides…
A: Biohazard is defined as any biological substance that can pose or cause a threat and harm to biotic…
Q: Gram Stain Results: Staphylococcus aureus (a Gram-positive coccus) Escherichia coli (a Gram-negative…
A: Staining is the method of producing different colours that obtained by the reaction of reagents with…
Q: microbiology
A: For to obtain cultures and Sensitivity faster, depends on targeted bacterial strain. The media…
Q: When viewed under a microscope, bacteria appear as red rods after they have been stained. Explain a…
A: Introduction Bacteria Are Minute, Single-celled Organisms That Can Be Found In Large Numbers In All…
Q: what is the gram staining procedure for e-coli
A: Gram staining is the laboratory technique which is used to differentiate between the morphology of…
Q: Create a flowchart using the given reagents and equipment to perform bacterial DNA extraction. ●…
A: Introduction : DNA extraction is a very skilled process and it requires these four steps in…
Q: I missed a couple of my labs due to covid and am struggling to teach myself how to do serial…
A: The last two years have been extremely difficult because you can still learn and understand the…
Q: Explain how to perform a serial dilution
A: Serial dilution is stepwise dilution of a solution keeping the dilution factor constant in each…
Q: You and a partner conduct the acid-fast staining using the same two bacteria. You know that there…
A: Acid-fast staining is a differential staining technique that proves to be an important diagnostic…
Q: What is the order of staining in microbiology
A: Crystal Violet - Primary stain ( Stain the cell wall of the microorganisms) Gram's iodine - Acts…
Q: Describe three problems associated with using the standard plate count method for determining the…
A: Typically, an accurate count of bacterial colonies is required for biological processes. Microbial…
Q: Based on the photo, describe the bacteria observed from the different staining technique. -…
A: Microbiology was a blessing in the eradication of mass-destructive epidemics such as rabies,…
Q: lain how a wire loop and wire needle is used in microbiology laboratory.
A: A laboratory dedicated to the culturing, examination, and identification of microorganisms including…
Q: Why was knowledge of how to obtain a pure culture important for development of the science of…
A: A pure culture is one that contains only one species of an organism. The procedure of pure culture…
Q: What technology is available for faster bacterial culture?
A: BASIC INFORMATION BACTERIA It is one of the type of biological cell. They belong to prokaryotic…
Q: Does fructose can produce a positive result in Seliwanoff Test? Elaborate your answer
A: Seliwanoff’s test is a test for carbohydrates that can distinguish between aldose and ketose sugars.…
Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…
A: Serial Dilution -- Serial dilution are used to calculate the concentration of microorganism .It…
Q: trospray mass spectro
A: The amino acid of any of a group of organic molecules that consist of a basic amino group (―NH2), an…
Q: If you had a liquid culture containing three different species of bacteria, how could the use of a…
A: A microbiological culture, also known as a microbial culture, is a technique for multiplying…
Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…
A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…
Q: What is gram staining Step by step procedure Explain its basis and how it differentiate bacteria use…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40…
A: In the question it is asked that they want to plate out 40 bacteria, and this count comes under the…
Q: How do you make bacteria in the laboratory?
A: Bacteria are microscopic single-celled organisms that thrive in diverse environmental conditions.…
Q: What are the advantages and limitations of studying bacteria by means of the Culture method?
A: Introduction Culture:- It is a method of growing microorganisms, tissue cells, or other living…
Q: What is the principle of Grams staining based on the cell wall composition of bacterial cell.
A: Bacteria are a kind of organic cell. They comprise an enormous area of prokaryotic microorganisms.…
Q: You perform the Methyl-Red (right) and Voges-Proskauer test (left). Interpret the results. Which…
A: Voges-Proskauer (VP) is a test used to determine the acetoin in broth culture of bacteria. VP broth…
Q: Explain why an isolated colony and a pure culture are not the samething.
A: The isolation techniques: It consists of separation of bacterial cells from other cells from mixed…
Q: What are the general rules in grams staining classification of bacteria?
A: In microbiology, Gram staining is the most widely utilised, vital, and widely used differential…
Q: staining method would you use to verify that the isolates are not Mycobacterium species? Describe…
A: Staining is a method of coloring biological tissues to increase the contrast. This technique is…
Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…
A:
Q: How to make a bacterial cell competent
A: Bacterial cells can be made competent either naturally or artificially. Competent cells are cells…
Q: how else could bacteria in soil be characterized and identified other than gram staining
A: Gram staining method is one of the important ways to identify bacteria. This principle is based on…
Q: Explain, in your own words, why it is important to perform serial dilution when trying to quantify…
A: Serial dilution is type of dilution used in field of microbiology to identify the cell count.
Q: Exaplain in your own words how to Accumulate data and characteristics of the unknown until a…
A: Microorganisms like bacteria are smaller in size such that they are not visible through the naked…
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Solved in 2 steps
- Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series? Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.How do you make liquid bacteria culture?What if the purpose of bacterial culture? Why might it be useful to grow bacteria?
- how to find unknown bacteriaIf you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40 bacteria what would be the dilution scheme of using 4 dilutionsWhat is the purpose of staining bacteria? What is the purpose of collecting bacteria from different sources and staining them. What can you learn from this type of experiment?
- What is gram staining Step by step procedure Explain its basis and how it differentiate bacteria use correct terminologyexplanation on how a bacteria should be isolated from the infected organism and can be grown in pure culture .You perform the Methyl-Red (right) and Voges-Proskauer test (left). Interpret the results. Which methods would you use to determine is the bacterial sample is E. coli or P. vulargis? What results would help you to decide?