Q: Using the TABLE shown, identify which proteins would elute together in a gel filtration experiment.…
A: Chromatography is a laboratory technique used for the separation of solutes from the sample mixture.…
Q: Explain this example of the following water quality serial dilution. Note the dilution factors and…
A: Serial dilutions are done regularly for environmental samples expected to contain high bacterial…
Q: What are the pros and cons of serial dilution method and W/V % method?
A:
Q: Explain the purpose of thiosulfate in the medium.
A: Culture media is used to enrich the number of bacterial growth, to select certain bacteria and…
Q: James inoculated a species of bacteria into KIA media and realized that it has a yellow butt and a…
A: This question is about identification of lactose non fermenting strain which is to be grown in…
Q: Find out what the various components of the medium used for propagation of an explant in vitro are?
A: Plant tissue culture is a technique through which plant cells, tissues or plant parts are grown on…
Q: What is the purpose of the baking soda solution in the leaf disks experiment?
A: Leaf disks experiment is an experimental set up which is aimed at studying the effects of various…
Q: Explain the principle involved in O.D determination of microbial populations using the…
A: A spectrophotometer is an instrument that can determine the concentration of a compound or solution…
Q: Describe how you would prepare the required dilution series using the least amount of dilution water
A: Dilution is the process by which water is added to a solution or a chemical to make it less potent.…
Q: What salt did Meselson and Stahl use for their ultracentrifuge gradients? O NaCI O NH4CI O CSCI O…
A: Given: DNA replicates semiconservatively. Matthew messelson and Franklin stahl performed an…
Q: epare 100 cfu/mL bacterial suspension. Present in diagram if necessary and show all calculations…
A: A colony-forming unit, or CFU, is a measurement of microbe concentration in a test sample. To get a…
Q: setting up an experiment to support the hypothesis that "E. coli will grow best at a neutral pH"…
A: Microbiology is the study of microorganisms under the microscope. Microorganisms are bacteria,…
Q: Give an example of an industrial/clinical setting where quantifying viable bacteria would be useful.…
A: Quantifying viable bacteria is useful :-
Q: Design an experiment to isolate microorganisms able to degrade starch. Explain step wise
A: Starch is a polysaccharide present abundantly in plants and generally deposited in granule form in…
Q: how many bacteria/ml of the diluted broth would be present on average
A: 37 bacteria/ml of the diluted broth would be present on average.
Q: Would heating the culture in a sealed, small-diameter tube that is totally immersed in a water bath…
A: Microbial culture is a process of multiplication of microbial organisms by allowing them to…
Q: What would be the independent variables for the spinach leaf serial dilution experiment
A: The spinach leaf serial dilution experiment is a method, used to measure the rate of photosynthesis…
Q: Describe how to measure the effectiveness of a chemical disinfectant.
A: A chemical liquid or a substance that is used to kill microbes is referred to as a disinfectant.…
Q: When viewed under a microscope, bacteria appear as red rods after they have been stained. Explain a…
A: Introduction Bacteria Are Minute, Single-celled Organisms That Can Be Found In Large Numbers In All…
Q: How to prepare wet mounts and hanging drop mounts of live specimen in microbial motility?
A: Wet mounts and hanging drop mounts of live specimen are used in microbial motility.
Q: What are three pieces of information that you can obtain from observing the growth of bacteria on…
A: Solid agar is used as a nutrient media to culture bacteria. Agar is composed of linear…
Q: What are the difference in the movement of microorganisms under wet mount from hanging drop…
A: There are some plus point for both the techniques like the wet Mount and hanging drop method…
Q: What is the reagent used to detect glucose in the ph and temperature experiments? What is its…
A: Presence of Glucose is detected using Benedict's Test, Fehling's Test, and Barfoed's Test. Presence…
Q: I missed a couple of my labs due to covid and am struggling to teach myself how to do serial…
A: The last two years have been extremely difficult because you can still learn and understand the…
Q: How many microliters of a 2.5X restriction enzyme buffer would be required if the total volume of…
A: Restriction enzyme buffer: Buffer selection is very important for obtaining the high activity of…
Q: Explain how a recrystalization will purify a solid. You MUST use relevant drawings in your answer.
A: Recrystallization: It is a technique performed in the laboratory to purify solids based on their…
Q: Identify different ways to dilute. Learn how to solve a dilution problem
A: In a solution concentration of solute is reduced simply by mixing more water or by adding more…
Q: briefly explain the principle behind this technique; gel-filtration chromatography
A: First of all, let's discuss gel-filtration chromatography. This technique is also known as the size…
Q: Describe how to most easily calculate the generation time for a population of bacteria in a batch…
A: Bacteria grow when one bacterial cell divides to form two daughter cells. Thus, bacterial growth can…
Q: Supposed you are to work in your experimental research, with the first step of establishing presence…
A: Spectroscopy is the study of the absorption and emission of light and other radiation by matter, as…
Q: Define water activity and briefly describe how it can be determined. Why is it difficult for…
A: The tiny organisms that cannot be observed by unaided eyes are the microorganisms. The microbes…
Q: a. What is the function of the SDS in the lysis buffer? the NaOH? b. What does the potassium…
A: These question are regarding plasmid DNA isolation
Q: List down two important precautions in conducting brine solution experiment.
A: Brine solution experiment : The brine solution experiment is generally used to study the process of…
Q: Calculate the doubling time of a culture of E. coli that is started with 3x105 CFU/mL and grows to…
A: Given: No = 3x105 CFU/mL (number of bacteria at the beginning of a time interval) Nt = 4.6x108…
Q: What are the main types of immobilization techniques used in biosensors? (with a deep explanation)
A: Immobilization It is a technique that is used for the chemical and physical fixation of the…
Q: Discuss the functions of the following components in performing gel electrophoresis;…
A: Introduction: The technique of gel electrophoresis is used to separate DNA fragments based on their…
Q: Illustrate the dilution series used and label the final dilution of each dilution.
A: The serial dilution method is regularly used in the microbiology lab. In environmental samples like…
Q: Need answer of this question Identify the principles and procedures related to disinfection and
A: As we know Sterilization is the process of killing microorganisms along with their spores. It is the…
Q: a) What dilution is created when you dilute 1mL of bacteria into 99mL of water? 10 What is the…
A: The stock solution is the more concentrated solution, while when one adds the solvent to it, then it…
Q: What is Bioreactor? What are the advantages of Stirred tank Bioreactor over Shake flask.
A: Large-scale production of organic substances such as antibiotics, hormones, vitamins, acids,…
Q: Why do we use turbidity as a method to measure bacterial growth? How does it work? How do you…
A: Turbidity measurement is one of the methods to measure the microbial cultures, helps determine the…
Q: What are two advantages of filtration in microbial control? Explain how the filtration process…
A: According to the question, we have to describe two advantages of filtration in microbial control. In…
Q: WHAT IS THE CONNECTION OF BACTERIAL CULTIVATION TO MICROBIAL PHYSIOLOGY
A: Microorganisms are simple living organisms that can be observed through a microscope. The common…
Q: Briefly describe how you would make 500 mls of a 1X electrophoresis running buffer from a 15X stock…
A: Electrophoresis buffer: Electrophoresis buffer can be Tris-acetate EDTA (TAE) or Tris-borate EDTA…
Q: Make a schematic diagram of the procedure of Barfoed's Test which is also a test for reducing
A: Carbohydrates are polyhydroxy aldehydes or ketones which are associated with reducing properties.…
Step by step
Solved in 2 steps with 1 images
- Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series? Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration. show solutionDesign a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration.
- Devise a serial dilution scheme to prepare a 10-5 dilution on a plate using the least number of sterile water dilutiontubesIf a broth culture of bacteria, initially containing 37,000 bacteria/ml, were diluted to a 1:1,000 dilution, how many bacteria/ml of the diluted broth would be present on average? Show your solutionsYou dilute 1 mL of bacterial culture with 99 mL of water. What is the resulting dilution factor?