Create a flowchart using the given reagents and equipment to perform bacterial DNA extraction. ● Tris-HCl buffer ● Lysozyme  ● Liquid nitrogen ● Dishwashing liquid ● Rubbing alcohol ● Mortar and pestle   ● Meat tenderizer

Q: Which of the alternatives below indicates techniques used both to purify and to concentrate…

A: Proteins are polymers of amino acids linked by peptide bond and it is one of the important…

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…

Q: Find the amount of dehydrated medium needed to prepare 15 BUTTS, 20 SLANTS, and 25 BUTT-SLANTS.

A: Slants, Butt-slants, and butts are different types of media prepared for bacterial growth. Slants…

Q: tate the apparatus used view small samples

A: The world is full of organisms. Organisms have developed from minute single celled structures to…

Q: Why would the optical tweezers be a method superior to dilution and liquid enrichment for obtaining…

A: Optical tweezers have been used to trap dielectric spheres, viruses, bacteria, living cells,…

Q: How are clinical chemistry test results used to confirm or rule out a presumptive diagnosis? Why is…

A: Any medical test's primary goal is to assess if a patient does or does not have a specific disorder.

Q: Since the development of the calcium chloride transformation procedure, other procedures have been…

A: Bacterial transformation is the transfer of  DNA released from a donor bacterium into the…

Q: Fill in the blanks with appropriate terms, phrases, or clauses to complete the following sentences…

A: Electrophoresis is the process of separation of charged molecules on application of electric field.…

Q: How would you identify this unknown bacteria using a flowchart and the bacteria below as a possible…

A: Biochemical tests in Microbiology help in distinguishing and identifying bacteria. Through the…

Q: Koch's postulates include all of the following EXCEPT O 1) Isolate the organism in pure culture. The…

A: Koch's postulates are a series of findings and experimental criteria suggested in the late 1800s by…

Q: Practical techniques used to identify unknown microorganisms’ You should include the types of tests…

A: Microbiology is the study of microorganisms, identification, and classification of a particular…

Q: In animal cells, is used to distinguish between living and dead cells when counting. Select one: a.…

A: Introduction:-  Trypan blue is a dye that is used to count live cells by identifying only dead…

Q: Which of the following was added to cell lysate to stabilize RNA molecules during the RNA…

A: RNA Extraction is the process of purification of RNA from biological samples. A Trizol Reagent is…

Q: Which reagent removes contaminant proteins from DNA solution? Sodium dodecyl sulfate (SDS) O Sodium…

A: A major goal of the nucleic acid isolation is the removal of proteins . The seperation of nucleic…

Q: Put the numbers in the space provided to indicate the order of Capsule Staining. If the statement is…

A: The primary function of capsule staining is to differentiate capsular material from bacterial cells.…

Q: Given the information provided, please answer the following question: Volume of cell suspension…

A: For setting up the new culture of the cells, the volume of cell suspension required is to be…

Q: Provide a brief description and state the function of the following tools and equipment used in the…

A: A laboratory is a facility that provides controlled conditions in which scientific or technological…

Q: Comment on the boiling technique for nucleic acid extraction? Why is it done?

A: DNA extraction involves three main steps. They are, Cell disruption. DNA extraction. DNA…

Q: Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the…

A: The wet mount is the most basic method of preparation, in which the specimen is placed on the slide…

Q: Ninhydrin Test Samples used:  Egg Albumin Gelatin Dispersion Added reagent: Ninhydrin Solution…

A: Ninhydrin is a general test for proteins and amino acids. It degrades amino acids into aldehydes,…

Q: A gel shows undigested lane 3000 bp while digested lane shows BS 2000 and repeat 1000. what does…

A:

Q: I missed a couple of my labs due to covid and am struggling to teach myself how to do serial…

A: The last two years have been extremely difficult because you can still learn and understand the…

Q: During the lumbar puncture 3 bottles are used: Why don’t they spin the sample that will be used for…

A:

Q: Which technique is preferred in the separation of fatty acyl methyl esters from a mixture?a)…

A: Chromatography is defined as the physical method that can be used for separating different…

Q: Four amino âĉid samples were subjected to a reverse-phase chromatography set up and yielded the…

A: Retention factors (Rf) values are important since they determine the differential separation of the…

Q: Describe three problems associated with using the standard plate count method for determining the…

A: Typically, an accurate count of bacterial colonies is required for biological processes. Microbial…

Q: Chromatography technique works on the principle of

A: Chromatography is a technique used for separation of mixture by using a moving solvent.

Q: Which of the following was added RNA extraction? Buffer RPE Wash buffer guanidine-isothiocyanate…

A: DNA molecule is a sritical methd. It helos in serine,  nRNa Nither momt tertibre

Q: In which reagent is extracted DNA suspended to put it in solution? O Sodium dodecyl sulfate (SDS)…

A: Deoxyribonucleic acid is a polymer made up of two polynucleotide chains that coil around one another…

Q: trospray mass spectro

A: The amino acid of any of a group of organic molecules that consist of a basic amino group (―NH2), an…

Q: Why annealing, denaturation temperatures are different?  Please answer at your own words.

A: Question: Why annealing, denaturation temperatures are different?

Q: Nucleic acids usually migrate towards the negative electrodes. Validate the statement.

A: NUCLEIC ACIDS- They have either single or double-stranded DNA or RNA molecules. The nucleic acids…

Q: Write the instructions, reagents, principles and the procedures for the following.  1. Periodic acid…

A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…

Q: Describe in no more than ten sentences what occurs during denaturation

A: Biomolecules are organic macromolecules that play an important role in the proper functioning of the…

Q: If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40…

A: In the question it is asked that they want to plate out 40 bacteria, and this count comes under the…

Q: Quadrant streak plate Pure culture T-streak plate Zig-zag streak plate Environment sample Pure…

A: Streak plate method is used for the isolation of a single bacterial colony in microbiology.

Q: When a sample presents some matrix interference, which of the ff. are carried out? Dilute sample to…

A: shift in analytical results caused by one or more specific constituents in the matrix, generally…

Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?

A: Introduction  We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…

Q: One of the following is a physical denaturation of proteins except: X-ray irradiation Vigorous…

A: Protein denaturation is a wide concept and dependent on multiple factors. whenever the protein is…

Q: Use the dilution scheme to answer. The concentration of bacteria in the original sample is CFU/ml.…

A: The Entire Viable Count refers to the total number of colonies (TVC). The cfu/ml (colony forming…

Q: Write down the materials, reagents, Principle, procedure  and results for Ziehl-Neelsen stain

A: It is a differential staining technique which was first developed by Ziehl and later on modified by…

Q: Which of the following methods for determining protein concentration is most commonly used? O Lowry…

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE…

A: A serial dilution is the stepwise dilution of a substance in solution. Normally the dilution factor…

Q: To visualize individual atoms in a protein or nucleic acid you would use: X-ray crystallography…

A: Crystals of many proteins and other biomolecules causes the diffraction of x-ray beam. The…

Q: Complete the table below by adding (+) or (–) if each of the sample (amino acid or protein)below…

A: biuret test:            Compounds with peptide bonds forms purpule colour when treated with biuret…

Q: Congo red is a red acidic stain and methylene blue is a l a bacterial smear with a mixture of eosin…

A: Eosin Methylene Blue (EMB) agar is both selective and differential culture medium. It is a selective…

Q: To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a…

A: Given Values: Volume of the 16 hour culture = 500 μl Final Volume of the culture media = 5 ml

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.