What is the purpose of adding 10mM of Tris-HCL (pH 7.5) during the extraction process? a. It ensures that the DNA that is released from the cell is turned into single stranded format. b. It denatures the proteins that were released from the cell.  c. It breaks down the cell membrane to release the DNA. d. It neutralizes the the lysis reaction.

Q: 1. How does real-time polymerase chain reaction work? 2. How is SYBR Green used in the experiment?

A: Solution: Answer: 1) How does real-time polymerase chain reaction work? Definition of real-time…

Q: Which amino acid listed in the pKa table may behave as a zwitterion at a pH of 7.0-7.4?

A: An amino acid acts as a zwitterion at its isoelectric point.

Q: Write the K, K2 and Kg reactions for arginine showing the structures of each form of argenine acid.…

A: Structure of arginine with the respective pka values of ionizable groups is:

Q: the same 10.00 mL of a 0.5 M NaOH is added to 150.0 mL of MOPS buffer. The buffer contains 0.500M…

A:

Q: Five students performed a Kjeldahl nitrogen analysis of a protein sample. The weight % nitrogen…

A:

Q: CH2 он Tyrosine Draw the structures of tyrosine as different pH values. Please refer to the lecture…

A: Solution -

Q: 6. The end of a new nucleotide is always added to the end of an existing strand. 7. Distinguish…

A: DNA is basically a genetic material and transfer genetic information necessary for synthesis of…

Q: HO 9. In the DNA double helix, why are purine bases on one DNA strand never paired with other purine…

A: Deoxyribonucleic acid (DNA) is a molecule made up of two polynucleotide chains that coil around with…

Q: 2. The a chain of haptoglobin exhibits genetic polymorphism since glu- tamic acid can be replaced by…

A: 2)First, we will see the difference between the SDS and Native polyacrylamide gel electrophoresis…

Q: How would you make 2.0 L of electrotransfer buffer for transferring the proteins from a gel onto…

A: The composition of electrotransfer buffer is given as 1) Concentration of Tris (this is a solid; MW…

Q: 1. Will both DNA and RNA give a positive test with nitric acid? Why?

A: DNA carries heridetary material, whereas RNA is known for making protien and genetic material source…

Q: viii) You have the following stock solutions available: 2.5M Tris, 0.5M Glycine and 10% SDS, how…

A: To prepare 1 liter solution of 25mM tris, 190mM glycine and 0.1% SDS can be prepared by following.

Q: alculate the Ksp for uraninite (reaction 1) and rutherfordine (reaction 2).             UO2 + 4 H+…

A: UO2+4H+ →U4+  + 2H2O Ksp = U4+Ksp = S Reaction  :1 Ksp  = S

Q: In iodometry, a student forgot to add H2SO4 before the addition of KI.   Is there a positive…

A: In iodometry, iodine is liberated by adding sulphuric acid to the solution of iodized salt.

Q: What additional single piece of information do you need in order to determine how much AP is being…

A: Answer: option c

Q: Human DNA contains approximately 30.4% A. Estimate the percentages of G, C, and T and compare them…

A:

Q: What is the Kb of a base whose pKa is 8.9?

A: The question is based on conjugate acid base principle. a strong acid has a weak conjugate base and…

Q: 1. Comment on the similarities and differences between X-Gal and indigo. What purpose do the halogen…

A: As per the guideline, since you have asked multiple questions, we have solved the first question for…

Q: what is Edman’s degradation? How will you break disulfide bonds in proteins?

A: A scientist named Pehr Edman discovered the process of Edman's degradation.

Q: Write the expression for the Ka of cysteine. Remember to use the acid dissociation reaction in water…

A: Here we are to find the expression of the dissociation of weak acid cysteine

Q: pectin gel formation.

A: Pectin formed by hydrogen bonding between the corboxylic group of acids and hydroxyl group OH of the…

Q: In the experiments of Barrick et al. (as shown), it was observed thatreplacement of histidine by a…

A: Hemoglobins have two alpha chains and two beta chains. When oxygen binds hemoglobin, there is a…

Q: Pls answer the following questions 1.What is the action of invertase? 2.What is the reagent used to…

A: Invertase is an enzyme which helps in hydrolysis of disaccharides sucrose to give glucose and…

Q: Five students performed a Kjeldahl nitrogen analysis of a protein sample. The weight % nitrogen…

A: Given values = 15.5, 14.0, 15.7, 15.4 15.8  We have to find Gcalc and tell whether outlined should…

Q: If the Ksp of Ca(IO)2 is 4 x 10-9 and the [Ca2+] is found to be 0.001M, then the [103'] is: O 6.5 x…

A: Since you have asked multiple type questions, we will solve only first  questions for you. If you…

Q: A) 30mL of a 0.2M solution of tripeptide Glu-Lys-His is adjusted to pH=9.5. The solution is then…

A:

Q: Explain the relationship between Piscirickettsiaceae and Which of the two bacteria may not have been…

A: The Piscirickettsiaceae belong to the family of proteobacteria. Almost, all the species belong to…

Q: How can we quantify the amount of energy required to generate a concentration gradient (Figure…

A: Gel electrophoresis s a technique used to separate DNA fragments based on their size. Samples are…

Q: What is it ACTIVITY 4B. Matching type. Match the terms in column A to column B. Write your answer on…

A: Carbohydrates  - are classified  into two classes  1) sugar - these are crystalline substances which…

Q: How do we prepare an RNA sample from yeast? Whats the purpose of heating the yeast with 10% NaOH?

A: DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) are genetic material in the living cells. DNA…

Q: Which of the following scenarios DOES NOT point to the procurement of an assay? A. a certain type of…

A: An assay includes an analytical procedure through which a sample is checked qualitatively or…

Q: Given the following peptideSEPIMAPVEYPK(a) Estimate the net charge at pH 7 and at pH 12. Assume the…

A:

Q: When the relaxivity of a Gd-based magnetic resonance agent is high, this means that slight changes…

A: The given statement is False. Slight changes in Gd concentration will result into big changes in…

Q: Calculate the Ksp for uraninite (reaction 1) and rutherfordine (reaction 2).             UO2 + 4 H+…

A: Solubility balance is a kind of complex balance that occurs when a solid chemicals compound is…

Q: Solve this

A: Proteins are formed by condensation reaction of amino acid in which by product such as water is…

Q: What is Depurination ?

A: DNA is a molecule carrying the genetic information. It has a double helical structure containing two…

Q: 1.What is the reagent used to detect glucose in the detection of invertase? What is its principle?…

A: The main function of invertase enzyme is to break down of sucrose into fructose and glucose .…

Q: What are annulenes ?

A: The compounds containing carbon and hydrogen only are termed as hydrocarbons. Hydrocarbons are…

Q: The recommended working buffer for the restriction enzyme Notl is compe 10 mM MgCh 100 mM NACI 50 mM…

A: Tris HCl is an organic compound often used in buffer solutions such as TAE or TBE for…

Q: What does high NaCl do to protein that is bound to a Sp column?

A: Column chromatography: Column chromatography is used to separate charge analytes. Column…

Q: This technique uses the principle of isoelectric point to separate a mixture of proteins into its…

A: 1. Electrophoresis : Neutral amino acid contains one -COOH and one -NH2 groups. Hence, they exist as…

Q: Seriously, You can't figure this one out. 日區 eLectro blule er TATIAAT Down: Across: 1. Source of RFP…

A: 1)DsRed 2) E coli 3) 4) Exonuclease 5) Enzyme 6) Gel Electrophoresis 7) Restriction Enzymes 8)…

Q: Pick all that are TRUE regarding analysis of quaternary structures of proteins using polyacrylamide…

A:

Q: 1. A suture is made of polylactide, after 30 wee percentage of the original mass remaining?

A: Poly-L-lactide (PLLA) is one of the most significant members of a group of polymers regarded as…

Q: 10. Draw the full chemical structure of the tetrapeptide G-H-F-A as it would look at pH 12.0. Use…

A: We have to predict the structure of peptide at pH of 12

Q: P UTF-8 Chapter 12 Solutions-20 x+…

A: Given-> Initial Volume = 2.0 L Initial molarity = 6.0 M Final volume = 6.0 L On dilution moles…

Q: What is the pI for the polypeptide ALRHEN? Use pKa of 8 for the N-terminus and 4 for the C-terminus

A: