viii) You have the following stock solutions available: 2.5M Tris, 0.5M Glycine and 10% SDS, how would you prepare a 1 Litre solution of 25mM Tris, 190mM Glycine and 0.1% SDS for use in a protein separation experiment.
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A: At lower pH that means in acidic conditions the carboxylate ion takes up proton and exists as…
Q: A typical amino acid with one amino group and one carboxylic acid group, such as serine can exist in…
A: At lower pH that means in acidic conditions the carboxylate ion takes up proton and exists as…
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Q: A sample containing histidine (pKas 1.8, 6.0, 9.3), lysine (pKas 2.2, 9.1, 10.5), and leucine (pKas…
A: Because a protein's net charge is pH-dependent, the sample may be eluted from IEX medium by varying…
Q: A purified protein is in a HEPES (N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)) buffer at…
A: Given: concentration of NaCl = 600 mM Since, 1 mL of sample, 600 mM NaCl and HEPES buffer is…
Q: Consider an amino acid with the following pKa values: pKa1 = 2.71 and pK, = 9.31 At what pH value in…
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Q: Tyrosine gave a positive reaction to these tests except l. Hopkins-Cole |I. Biuret I.Sakaguchi IV.…
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A: A question based on absorption that is to be accomplished.
Q: Use the following chromatogram of standard amino acids: 24 16 19 21 12 15 6,6 9 10 23 13 14 17 18 10…
A: In chromatography, a higher retention time is linked to a higher affinity for the stationary phase…
Q: The electrophoresis of a sample containing 2-amino-4-methylpentanoic acid. (pKa of a-COOH = 2.2, pKa…
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Q: A student performed a chromatography experiment to determine the Rf value of an unknown amino acid.…
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Q: Find the isoelectric and zwitter ion of lysine
A: isoelectric point means the characteristic pH at which net effective charge of an amino acid is…
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Q: Which most accurately describes what the diagram below represent? Reflected light Incident light…
A: It's a Schematic diagram of an SPR based genosensor. The incident polarized light is coupled by a…
Q: they have a binding site for X with a Kd (dissociation constant) of 10⁻⁵and 10⁻⁸ M, respectively.…
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Q: A protein with a pI of 7.5 will be purified using ion-exchange chromatography. Which ion exchange…
A: b) A resin with sulfonic acid group is the correct option.
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A: Idometric titration:
Q: which of the following chromatographic methods is best used for separating amino acids?
A: Chromatography is the technique that is used for the separation of different components from the…
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A: The given data is as follows. A1 = 0.396 (first point) A2 = 0.599 (second point) C1 =…
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A: As per the bartleby expert guidelines, I am allowed to answer one question at a time . Please…
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A: Isoelectric focusing: It separates the different amino acids based on their differences in their pI…
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Q: Question attached
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A: Concentration of HRP sample = 2.0 mg/mL
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A: Here we are required to find the pH of weak base solution.
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A: Here, we are titrating a tetrapeptide Arg-His-Ile-Glu against HCl.
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A: As there are three question are present I answered only one. you can repost your remaining two…
Q: 1) Compare between three steps for Kjeldahl method for determination of protein?I
A: INTRODUCTION: Kjeldahl method is defined as the method which is used for quantitative determination…
Q: A 5.00 mL sample of filtered orange juice was treated with 50.00 mL of 0.01019 M I3– to oxidize…
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Q: what are the advantages of mediator-based amperometric enzyme sensors for glucose compred to…
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Q: A 5.00 ml sample of filtered orange juice was treated with 50.00 mL of 0.01019 M I3 to oxidize…
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A: Given-> Initial Volume = 2.0 L Initial molarity = 6.0 M Final volume = 6.0 L On dilution moles…
Q: A purified protein is in a HEPES (N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)) buffer at…
A: Initial concentration of NaCl solution, Minitial = 600 mM Initial volume taken, Vinitial = 1 mL…
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A:
Q: 13.) A mixture containing 50% mole of A is distilled at a temperature T, what is the distillate to…
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Q: You would like to calculate the concentration of a protein in a solution measured in a 96 well…
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- A juice concentrate was colorimetrically assayed using Nelson’s test. One milliliter (1.00 mL) of the sample solution and various concentrations of the standard glucose solution were treated with freshly prepared Nelson’s reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbances at 480 nm for distilled water, glucose standard, and for the sample are 0.052, 1.702, and 0.926, respectively. What is the reducing sugar concentration (mg/mL) in the juice concentrate? The equation of the line was plotted to be: y = 1.6656x - 0.0885 0.578 mg/mL 577.9 mg/mL 57.7 mg/mL 5.78 mg/mLA brewed tea brand sample was subjected to solvent extraction to extract caffeine. The cup of tea measuring 200 mL was extracted with petroleum ether. With KD = 20.0 (caffeine in pet ether-water), the % caffeine (194.10) extracted after 3 extractions with a total of 75.0 mL pet ether used is: A. 97.7% B. 28.6% C. 71.4% D. 2.33%The separation and purification processes given below and the method used against them are given. Which or which of these pairings are correct? I. Brewing of tea - ExtractionII. Purification of water impurities - ChromatographyIII. Separating olive pomace from olive oil while producing olive oil-DecantationIV.Petroleum fractions obtaining - Ordinary distillationV. Obtaining essential oils and using them in perfume making - Water-steam distillationA. I, II, III, IVB. I, II, III, IV, VC. I, IV, VD. I, II, III, VE. I, II, V
- 20 tablets containing the dibasic drug ethambutol hydrochloride (MW 277.2 g/mol) were powdered. 0.3354 g of tablet powder was dissolved in 10 mL of 2 M sodium hydroxide and was extracted with 5 x 25 mL quantities of chloroform. The combined extracts were evaporated and the residue was dissolved in 100 mL of anhydrous acetic acid. Non-aqueous titration was carried out using 1-naphtholbenzein solution as indicator and 0.1014 M acetous perchloric acid. Volume of acetous perchloric acid required = 14.2 mL, weight of 20 tablets = 3.367 g, state content of ethambutol hydrochloride per tablet = 100 mg. Calculate the % of stated content in the tablets.Acetophenone is soluble in diethyl ether, isopropanol, and nhexane. Identify and justify the most suitable solvent for UV- Vis characterization?A 18 g of unknown organic sample was dissolve in 756 mL of benzene. The boiling point of benzene was increased by 3.36oC. As the first step of analysis, determine the moecular weight of the unknow sample? Kb of benzene= 2.64oC/m Bb of benzene = 80.09 oC density of benzene = 0.874 g/mL at 25 °C Answer in whole number, no units required.
- A 5.00-mL sample of blood was treated with trichloroacetic acid to precipitate proteins. After centrifugation, the resulting solution was brought to pH 3 and extracted with two 5-mL portions of methyl isobutyl ketone containing the lead-complexing agent APCD. The extract was aspirated directly into an air/acetylene flame and yielded an absorbance of 0.569 at 283.3 nm. Five-milliliter aliquots of standard solutions containing 0.400 and 0.600 ppm of lead were treated in the same way and yielded absorbances of 0.396 and 0.599. Please calculate the concentration of lead in the sample in ppm.Which of the following could DECREASE the amount of the recovered crystals in the purification by recrystallization experiment? Collecting the recrystallized benzoic acid by filtration using a pre-weighed filter paper. Using a long-stemmed funnel in the hot filtration step. Cooling the filtered solution rapidly. Washing the recovered crystals using hot solvent. a. II, III b. II, IV c. II, III, IV d. I, II, III, IVA dermatologic lotion contains 1.25 mL of liquefied phenol in 500 mL. Calculate thepercent strength of liquefied phenol in the lotion.
- Next, 2.00 mL of 9.63 mM S was added to 10.00 mL of a solution of unknown concentration of X, and the mixture was diluted to 25.0mL. Chromatographic analysis of this solution produced peak areas of 4822 for X and 4099 for S. What was the concentration of 5 (in mM) of the 25.0mL solution?A 5.00-mL sample of blood was treated with trichloroacetic acid to precipitate proteins. After centrifugation, the resulting solution was brought to pH 3 and extracted with two 5-mL portions of methyl isobutyl ketone containing the lead-complexing agent APCD. The extract was aspirated directly into an air/acetylene flame and yielded an absorbance of 0.527 at 283.3 nm. Five-milliliter aliquots of standard solutions containing 0.400 and 0.600 ppm of lead were treated in the same way and yielded absorbances of 0.396 and 0.599. Find the concentration of lead in the sample in ppm assuming that Beer’s law is followed.3. Assume that in extraction from water into toulene, analyte A has distribution ratio of 10. A 20 mL portion of an aqueous solution of A is extracted with toulene. Which of thw following procedures will result in most efficient removal of A from the aqueous phase into toulene? (Please show the solution of the correct answer) A. 1 extraction with 20 mL of touleneB. 1 extraction with 40 mL of touleneC. 2 extractions with 20 mL of toulene eachD. 4 extractions with 10 mL of toulene each