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What is the purpose of adding dishwashing liquid into the banana mixture? What reagent is used in research labs instead of dishwashing liquid?

What is the purpose of adding dishwashing liquid into the banana mixture? What reagent is used in research labs instead of dishwashing liquid?

Human Heredity: Principles and Issues (MindTap Course List)
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter14: Biotechnology And Society
Section: Chapter Questions
Problem 9QP
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  1. What is the purpose of adding dishwashing liquid into the banana mixture? What reagent is used in research labs instead of dishwashing liquid?
Extracting DNA in 10 Easy Steps 1. Mush half of the banana in the zip bag for about a minute until all the lumps are gone and it looks like pudding. 2. Fill a cup with the hot water and salt. Stir to dissolve salt. 3. Pour the saltwater mix into the bag. Close the bag and very gently squeeze and move the saltwater and banana mush together. Do this for 30 to 45 seconds. 4. Add the dishwashing soap into the bag and gently mix the contents. Avoid making too much foam. Set aside for about 1 min. 5. Place the coffee filter (bandage gauge) in a funnel piaced on top of a test tube/ graduated cylinder 6. Pour the banana mixture into the filter a little bit at a time to avoid breaking the filter. Let it sit until all of the liquid drips down into the cup. 7. Remove and throw away the used filter. O NO COPY. 8. Tilt the glass and slowly add cold aicohol down the side of the cup. You want the alcohol to form a layer on top of the banana mix, staying separated, so be careful not to pour it too fast. Make a layer of alcohol that is 1-2 inches thick. 9. After the alcohol layer is set up, wait for eight minutes. You may see some bubbles and cloudy material moving around in the alcohiol. This material is DNA clumping together. 10. Use the wooden stirrer to start poking the cloudy stuff in the alcohol layer. Spin the stirrer it in place to start gathering the cloudy stuff. When you are done, take a closer look at the stuff on the stirrer. You are looking at DNA! 11. Answer the worksheet at the end of this activity
Transcribed Image Text:Extracting DNA in 10 Easy Steps 1. Mush half of the banana in the zip bag for about a minute until all the lumps are gone and it looks like pudding. 2. Fill a cup with the hot water and salt. Stir to dissolve salt. 3. Pour the saltwater mix into the bag. Close the bag and very gently squeeze and move the saltwater and banana mush together. Do this for 30 to 45 seconds. 4. Add the dishwashing soap into the bag and gently mix the contents. Avoid making too much foam. Set aside for about 1 min. 5. Place the coffee filter (bandage gauge) in a funnel piaced on top of a test tube/ graduated cylinder 6. Pour the banana mixture into the filter a little bit at a time to avoid breaking the filter. Let it sit until all of the liquid drips down into the cup. 7. Remove and throw away the used filter. O NO COPY. 8. Tilt the glass and slowly add cold aicohol down the side of the cup. You want the alcohol to form a layer on top of the banana mix, staying separated, so be careful not to pour it too fast. Make a layer of alcohol that is 1-2 inches thick. 9. After the alcohol layer is set up, wait for eight minutes. You may see some bubbles and cloudy material moving around in the alcohiol. This material is DNA clumping together. 10. Use the wooden stirrer to start poking the cloudy stuff in the alcohol layer. Spin the stirrer it in place to start gathering the cloudy stuff. When you are done, take a closer look at the stuff on the stirrer. You are looking at DNA! 11. Answer the worksheet at the end of this activity
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