Why is betadine a good DIY substitute for gram’s iodine or dissolved Potassium iodide which is normally used in the laboratory?
Q: What is the purpose of the alcohol (OH) in the gram staining process?
A: Usually, microbes are colorless, so it’s very difficult to see them even after magnifying under…
Q: What types of samples can be measured with Circular Dichroism? Can information be extracted?
A: Circulate dichroism is the difference in the absorption of left handed circularly polarised…
Q: what effect would destaining with ethanol for one minute have on your judgment of the gram reaction…
A: Gram staining is a differential stain which differentiate bacteria into two groups gram positive and…
Q: Why does motility media contain a lower amount of agar than ordinary agar?
A: Agar is a phyco-colloid polymer of galactose monomers obtained from red algae (Gelidium and…
Q: Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled…
A: Agar or agar-agar is a jelly-like substances obtained from red algae. Agar is a solidifying agent…
Q: What is the logic behind testing for E. coli to detect fecalcontamination of water?
A: Bacteria are unicellular prokaryotic organism that maintains a definite shape since it contains the…
Q: Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.
A: Escherichia coli is the gram negative bacteria.
Q: Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when…
A: The oxidase is a test used in microbiology to identify bacteria that produce cytochrome c oxidase.…
Q: If you perform the motility test on Motility Medium and hanging drop for Bacillus cereus and B.…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Organism 1 Organism 2
A:
Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…
A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…
Q: FOOD MICROSCOPY: STARCH IDENTIFICATION The following figures are the photomicrographs of the…
A: Starch is mainly found in a plant source, as starch is a storage material.
Q: Why are the streak plates for microorganisms in coconut water incubated at 7°C?
A: The benefits of streaking for isolation is to produce isolated colonies of an organism on an agar…
Q: What is the correct Gram reaction of the unknown bacterium growing on your MacConkey agar (Gram (+)…
A: MAC is a selective solid growth media in which the bacteria can be classified and observed on the…
Q: What is the EFFECT and RATIONAL of Molten agar mixed with bacterial suspension has temperature…
A: Bacteria can be cultured in either a liquid media or a solid media based on the purpose of culturing…
Q: The following data were obtained by incubating gram-positive bacteria in nutrient medium +…
A: Gram-positive bacteria are bacteria that test positive in the Gram stain test, which has…
Q: Which of the following can most likely increase the yield of recrystallized benzoic acid? CHOICES:…
A: Recrystallization is a method that is used for the purification of solid compounds by the removal of…
Q: ould be the effect on your judement of a Gram reaction of an organism if you stain a thick smear…
A: Staining is a process of the well-known structure of the microscopy technique for enhancing contrast…
Q: What are high level disinfectants? What is an example of one? What is the method of action and the…
A: Disinfectants are chemicals that are used to kill the microorganisms present in the surroundings.…
Q: You saw no color change in both the first step and second step of the Nitrate Reduction test. What…
A: A nitrate reduction test is a test done to test whether the Enterobacteriaceae member produces the…
Q: why do We need to Wait for the iron Agar to set and then Add another thin layer of 45°C Iron Agar?
A: The test that uses iron agar is the Triple Sugar Iron (TSI) Test and is used to determine the…
Q: Membrane filters
A: Membrane filter are made up of cellulose.
Q: why is it important that a small amount of inoculum be used in preparation of an agar slant
A: Agar is a jellylike substance derived from the purified cell walls of red algae. It is added to…
Q: What would you expect to see if you cultured Gram-positive, lactose-fermenting bacteria on MacConkey…
A: Given: What would happen if you culture gram positive, lactose fermenting bacteria in MacConkey…
Q: In a nutrient broth, where do microorganisms acquire their requirements for mineral elements like…
A: Microorganisms are grown in laboratories using culture media. Culture media contains all the…
Q: Why do the leaf disks in the baking soda solution (treatment) begin to float?
A: The baking soda solution used in floating disk photosynthesis demonstration. Release of Oxygen in…
Q: What is the purpose of the vial that does not contain chara? (the solution with chara turned…
A: bromothymol blue is an acid base indicator. It is blue if the solution is alkaline and it become…
Q: What is the purpose of iodine in the Gram stain? To act as a counterstain None of the other…
A: There are various staining techniques that are used to stain different cellular structures. The Gram…
Q: How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…
A: Bacillus subtilis is a very well bacteria that is widely used as a Gram-positive bacteria model…
Q: What makes agar good support for microbial growth?
A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…
Q: WHAT IS THE TYPE OF Growth patterns on Agar Slant and Broth Medium
A: Growth and morphogenesis of plant is determined by the composition of culture media. There are few…
Q: Do Gram-positive bacteria grow on PEA agar? If not, what prevents their growth?
A: Bacteria are common, mostly free-living organisms that are often made up of only one biological…
Q: Why do we add powdered zinc to the nitrate reduction test?
A: Bacteria are prokaryotic simple unicellular organisms. Their mode of respiration may be aerobic or…
Q: Of what practical importance are air borne microorganisms to the laboratory workers? What…
A:
Q: Which of the polysaccharides WILL DECREASE GELLING if acid is added to the sample? pectins…
A: Gels are solid, jelly-like structures made of colloid polysaccharides, proteins, and polymers…
Q: Why is it necessary to store the molten agar in a water bath at 50 degrees Celsius?
A: Introduction:- Agar is made up of two polysaccharides: agarose and agaropectin, with agarose…
Q: What type of agar is MacConkey agar?
A: MacConkey agar or MAC which is also described as a bacterial culture medium was named after the…
Q: Why should you filter some liquids for sterilization, rather than autoclaving them?
A: To control microbial growth or contamination there are a number of aseptic techniques developed that…
Q: what effect would using an old Gram's crystal violet preparartion containing crystallized dye have…
A: A microbe is a living entity that is so tiny that it cannot be seen with the naked eye. Microbiology…
Q: Which dye will not kill microorganisms if a wet preparation is made? Why?
A: Microorganisms are defined as organisms that cannot be viewed by the unaided eyes and are studied…
Q: What is diluent? Why is it useful for working with bacterial samples?
A: Dilution is done to decrease the concentration of the original sample that is being tested. Dilution…
Q: You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show…
A: Introduction :- A test to determine whether you have a bacterial infection is a bacterium culture.…
Q: Why should be avoided boiling more than 5 minutes in Barfoid test?
A: Barfoed test is carried out to detect the presence of monosaccharides in the sample. This test also…
Q: How many different types of colonies can you find on the blood agar plate? describe each. (As shown…
A: Blood agar is general medium that is provided with the nutrients and can be use as a general growth…
Q: Why did we add agar after we measure the pH?
A: pH means p stands for potential or power.H stands for Hydrogen atom.It is the power of the Hydrogen…
Q: Why do we use turbidity as a method to measure bacterial growth? How does it work? How do you…
A: Turbidity measurement is one of the methods to measure the microbial cultures, helps determine the…
Q: The soil bacterium Azotobacter can be enriched from soil using liquid media, which must contain the…
A: Azotobacter are nonsymbiotic bacteria. They are capable of fixing nitrogen aerobically.
Q: Please explain why is 70% ethanol used as an effective disinfecting solution in labs? Why not 95%…
A: Only 70% alcohol is effective as a disinfectant that is killing the microorganism on the surfaces.…
Q: What are the two components the medie must contain to test the bacteria for desulfurases
A: SIM (Sulfur, Iindole, Motility) medium differential medium is used mainly to test the ability of an…
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- Why would hydrogen peroxide be an effective disinfectant?Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when performing the oxidase test in microbiology lab?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?
- A disk-diffusion test using E. coli gave the following results: Disinfectant Zone of inhibition (diameter in mm) A 15 B 2.0 C 10 D 5 In the table above, which compound was the most effective against E. coli? A) A B) B C) C D) D E) The answer cannot be determined based on the information provided.In a nutrient broth, where do microorganisms acquire their requirements for mineral elements like Ca, Fe, etc. from? Can gelatin be used in culture media preparation? How is it different from agar? During sterilization, pH of culture media may change. How can this be fixed? How does pH affect the shelf life of unsterilized media?Which of the following can most likely increase the yield of recrystallized benzoic acid? CHOICES: A fluted filter paper was used during cold filtration. The receiving flask from hot filtration does not contain a small amount of solvent. Activated charcoal was not added to the solution. The solution was filtered immediately after hot filtration.
- Agar is a complex polysaccharide obtained from seaweed and cannot be digested by most microorganisms. Why does this property make agar an excellent solidifying agent for microbiological media?What are high level disinfectants? What is an example of one? What is the method of action and the advantages/disadvantages?If a less concentrated initial solution of socium bicarbonate was used in beaker C, would that solution require more or less bicarbonate to neutralize the acid? Why?