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How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?
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- What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?You prepared 10-7, 10-8, and 10-9 dilutions of a bacterial suspension in sterile saline. Then you plated 0.15mL if each dilution onto each of 3 plates of nutrient agar. After incubation, the colonies were way too numerous to counton the plates prepared from the 10-7 dilution. The plates from the 10-8 dilution had 355, 360, and 350 colonies. The platesfrom the 10-9 dilution had 115, 117, and 118 colonies. Determine the concentration of colony forming units (cfu/mL) inthe original bacterial suspensionYou are testing a river water sample. Your "original" plate shows 100 coliform colonies. Assuming you did the serial dilution correctly, how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.
- Your broth culture contains 471,612,048 bacteria/mL. Design a dilution series that would result in a plate with a countable number of colonies.Based on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on? *If 1.0 mL of a 1:1000 dilution produces 200 colonies on a culture plate, how many bacteria were present per mL in the original (undiluted) sample? 200,000 20,000 2,000 200
- What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 × 106 cells/mL?What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of an E-test strip.You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the undiluted milk, you do serial dilutions, and plate 0.5 ml of 4th dilution on agar. If you counted 78 CFUs from the 4th plate, how many bacteria/ml do you expect? (show solution) Do you think standard plate counts are very accurate? Why or why not?
- Why do you think the continuous culture has higher chances of contamination than batch and fed-batch culture?What is the difference between a mixed culture and a contaminated culture if they both contain more than one type of organism ?In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?
