An ion-exchange chromatographic separation is performed using a diethyl-aminoethyl- (DEAE)-sepharose column to separate proteins in a mixture. The mixture contains Protein A (pl=6.0), B (pl=5.0), C (pl=7.5), D (pl =1), and E (pl=4.0). The protein mixture is prepared in a buffer solution pH = 5. When the protein mixture is loaded onto the column, and the column is washed with a buffer solution pH 5, which protein(s) will be captured by DEAE-sepharose in the column? O Protein B because it is predominantly in net negative charge form. O Proteins D and E because they have predominately net negative charge in pH 5 solution O Proteins A, C, D and E because they have charges O Proteins A and C because both both predominantly have net positive charges O Proteins B and E because both predominantly have net positive charges
An ion-exchange chromatographic separation is performed using a diethyl-aminoethyl- (DEAE)-sepharose column to separate proteins in a mixture. The mixture contains Protein A (pl=6.0), B (pl=5.0), C (pl=7.5), D (pl =1), and E (pl=4.0). The protein mixture is prepared in a buffer solution pH = 5. When the protein mixture is loaded onto the column, and the column is washed with a buffer solution pH 5, which protein(s) will be captured by DEAE-sepharose in the column? O Protein B because it is predominantly in net negative charge form. O Proteins D and E because they have predominately net negative charge in pH 5 solution O Proteins A, C, D and E because they have charges O Proteins A and C because both both predominantly have net positive charges O Proteins B and E because both predominantly have net positive charges
Chapter1: Relative Value, Addition, And Subtraction Of Decimals
Section: Chapter Questions
Problem 39SST
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Transcribed Image Text:An ion-exchange chromatographic separation is performed using a diethyl-aminoethyl- (DEAE)-sepharose column to separate proteins in a
mixture. The mixture contains Protein A (pl=6.0), B (pl35.0), C (pl=7.5), D (pl =1), and E (pl=4.0).
The protein mixture is prepared in a buffer solution pH =5. When the protein mixture is loaded onto the column, and the column is washed
with a buffer solution pH 5, which protein(s) will be captured by DEAE-sepharose in the column?
O Protein B because it is predominantly in net negative charge form.
O Proteins D and E because they have predominately net negative charge in pH 5 solution
O Proteins A, C, D and E because they have charges
O Proteins A and C because both both predominantly have net positive charges
O Proteins Band E because both predominantly have net positive charges
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