You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing arginine at a concentration of 10 micrograms/mL. How much of the stock solution do you add to 1 liter of plate media?

You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing arginine at a concentration of 10 micrograms/mL. How much of the stock solution do you add to 1 liter of plate media?

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter9: Parenteral Medication Labels And Dosage Calculation

Section: Chapter Questions

Problem 3.3P

See similar textbooks

Related questions

Q: volumetric flask is made to prepare a certain chemical concentration through dilution for example if…

A: Asked : There is no volumetric flasks prepare 0.2N of acetic acid from 1 N acetic acid stock…

Q: If you run the Lugol's test on an unknown solution and you get a black color at the end, what does…

A: The use of Lugol's iodine reagent is used to differentiate starch and glycogen from other…

Q: What is Xanthan ? What is the importance of using Xanthan in the emulsion experiment?

A: Biopolymers are polymers, mainly polysaccharides, which are produced by microbes. They are mainly…

Q: How do you prepare 50 ml of 20 mg/ml proteinase K from 50 mg/ml stock solution

A: Proteinase K is a serine proteinase enzyme used to digest proteins. It is produced from a…

Q: If I want to make a 100mL buffer that has 2% SDS, 100mM tris pH 8, 10mM EDTA, and proteinase K…

A: Introduction: Buffer is defined as a solution that resists change in pH on the addition of acid or…

Q: 2 L of 5 mg/mL gelatin solution from 1 g/mL gelatin solution

A: Gelatin is a food ingredient that is derived from collagen. It is basically a protein in nature.

Q: Our laboratory has a stock of 50 X TAE (Tris-Acetate) and the laboratory manager asks you to prepare…

A: Gel electrophoresis is a standard molecular biology procedure used for the separation of DNA by…

Q: What are the mechanisms of samples separation work in Thin layer Chromatography?

A: Chromatography is a physicochemical technique for the division of compound mixtures, in view of the…

Q: Which of the following chromatographic techniques has an attached ligand on the stationary phase?…

A: Chromatography is a laboratory technique that is used to separate components, or solutes, of a…

Q: Using DEAE-cellulose as ion exhange resin, indicate the starting and ending pH for the narrowest…

A: Chromatography is a technique for separating different components of a sample, where the analyte is…

Q: A chromatographic separation of four compounds gave the following results. The dead time (tm) is…

A: Chromatography is a technique for separating a mixture in the laboratory. The combination is…

Q: In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?

A: Gel-filtration chromatography is a type of partition chromatography that is used to separate…

Q: If you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the…

A: Dilute formalin is used as a fixative in histological slides. The standard stock solution of…

Q: How will you prepare a 5% BSA solution? Show all your steps.

A: BSA stands for bovine serum albumin, which is a protein extracted from cows' serum albumin. In lab…

Q: What is the basis for the separation of proteins by the following techniques? (a) gel-filtration…

A: Chromatography is a separation technique of chemical substances into individual components. and the…

Q: Why is it important to select immiscible solvents when performing liquid-liquid extraction? In…

A: Liquid-liquid extraction is a process of separating components present in solutions by their…

Q: You receive an order for 750 mL of 27% dextrose solution. You have available D50W, 1000 mL. How many…

A: Given, D50W means 50% dextrose solution, 100 ml 750 ml of 27% dextrose is desired Sterile water

Q: For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much…

A: Here we are trying to prepare the stock solution from the raw red cell (RBC) solution. The parameter…

Q: hich compound is more likely to absorb at 200-

A: Amino acids Proteins are the polymers of nitrogenous compounds called amino acids. Each amino acid…

Q: Each liter of an intranasal antibiotic solution contains 160 mg of gentamicin. You have 2-mL…

A: A number of gram-negative infections are treated with the aminoglycoside antibiotic gentamicin.…

Q: 10mL of 1x solution from a 2x stock solution.

A: Dilution formula - C1V1 = C2V2 C1 = concentration of stock C2 = final concentration V1 = volume of…

Q: If you have an absorbance of 2.0 mL of stock dye, what kind of a dilution is needed to get it to…

A: Biochemistry is the science of study of the internal biochemical processes of organisms. The…

Q: In a polyacrylamide gel electrophoresis, the gel consists of specific gel components. name them

A: A type of gel electrophoresis (GE) that is used to quantify and separate desired protein molecules…

Q: To make a 1:100 dilution of a concentrated solution, you could O Mix 1 ml concentrated solution and…

A: Dilution in chemistry is the process of lessening the concentration power of a solute by adding in…

Q: If you had a 50 mL volume of 1% agarose gel to create with appropriate amount of SYBR Gold how would…

A: In molecular biology, agarose gel is used in electrophoresis to separate the DNA and RNA molecules…

Q: five components of an isoelectric focussing gel

A: The technique that separates proteins based on their isoelectric point (pI) are called isoelectric…

Q: Would a sample solution that has a high absorbance reading, have a low or a high concentration

A: Absorbance is a number that measures the diminishing of the transmitted radiant power in a solution.…

Q: In a spectrophotometer, what should be the appearance on the graph if the sample is pure? presence…

A: Spectrophometry is a widely used technique in identification and confirmation of chemical…

Q: How much agarose powder (g) would you use to make a 50 ml, 1.4% agarose gel?

A: Introduction:- Agarose powder is a linear polysaccharide that forms a gel when it is mixed with…

Q: Why absorbance is taken at 550nm in Biuret test? Does this absorbance change based on the detection…

A: In the UV-visible area of the spectrum, biological macromolecules including proteins and nucleic…

Q: You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis.…

A: TAE buffer used in agarose gel electrophoresis for the seperation of DNA and RNA.

Q: Which protein standard curve would you use to read off the protein concentration of an unknown…

A: Proteins are composed of twenty standard amino acids attached together via peptide bonds. Protein's…

Q: If you had a mixture of proteins with different sizes, shapes, and charges and you separated them…

A: Anode is an electrode through which the conventional current enters into a polarised electrical…

Q: o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the…

A: A working solution is different from Stock solution. A Working solution is prepared from stock…

Q: What will be the size of the larger product? O 5254 bp O 5192 bp O 5231 bp

A: Base Pairs -- Base pairs describes the relationship between the building blocks on the strands of…

Q: In protein electrophoresis, what reagent present in the sample buffer is used to eliminate…

A: Protein electrophoresis is a test used for the measurement of specific protein in a sample. The…

Q: Why do we need to place the lipid samples under direct heat after adding ethanol in a solubility…

A: The solubility test determines the presence of all lipids as a preliminary test.

Q: How will you know when the endpoint of the titration is reached? Why is it necessary to mix the…

A: Reaction It is a process in which reactant molecules react to each other and form another molecule…

Q: You dilute 1 mL of bacterial culture with 99 mL of water. What is the resulting dilution factor?

A: Dilution factor:In order to calculate the Dilution factor, the final volume is to be divided by the…

Q: what is the is the dilution factor when you add 2 mL ssample of 8 mL diluent?

A: Dilution is a process of changing the concentration of the solution by addition of solvent, which is…

Q: You are titrating an acid to determine the concentration of the base. The endpoint of neutralization…

A: Introduction In an acid-base titration, the equivalence point in a neutralization reaction indicates…

Q: If I had a standard with 2.0 mL of Bradford reagent then added 50μL of 250 μL/mL ; what would be the…

A: The Bradford protein assay is commonly used due to its sensitivity, speed, convenience, lack of a…

Q: He then ran the sample on an agarose gel and observed a strong band at approximately 400bp. What…

A: The band represent a small piece of DNA that was cut with restriction endonuclease and then…

Q: Dilute silver nitrate is sometimes applied to the eyes of newborn infants. Explain why a dilute…

A: Eye is an organ which helps us to provide vision it has several photoreceptors which receives light…

Q: What concentration of ethanol is considered optimum for disinfectant? Why not 100%?

A: Disinfectant refers to the chemical agents that destroy or inactivate microorganisms on an inert…

Q: What is the basis for the separation of proteins by the following techniques? (a) gel-filtration…

A: Chromatography is a technique in biophysics that determines three important steps of a mixture of…

Q: If the agar instructions say to mix 50 grams of agar in 1 liter of water, how many grams of agar…

A: 1 litre/ 1000 mL needs 50 grams of agar 1 mL needs 50/1000= 0.05 grams of agar 100 mL needs 0.05 X…

Question

Expert Solution

Step 1

In biochemistry, a stock solution is a high volume of a standard reagent. They're utilized for a variety of things. They are used to create essential materials in chemistry, biochemistry, and microbiology laboratories. To calculate the amount needed to prepare the requisite concentration significant for the experiment, one must be familiar with standard calculating methods.

Trending now

This is a popular solution!

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Similar questions

A 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?
Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?
If the agar instructions say to mix 50 grams of agar in 1 liter of water, how many grams of agar would you mix into 100 milliliters of water to get the proper concentration of agar?
If you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.
Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?
You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis. How much stock should you use?
2 L of 5 mg/mL gelatin solution from 1 g/mL gelatin solution
Draw the titration curve for an 800 mL 0.25 M solution of Arginine. (Graph pH vs. mole OH-.) Where onthe curve is the charge on Arginine +1.5? Identify it on the graph.
If equal portions of tretinoin gel, 0.1% w/w and 0.04% w/w are combined, what would be the resultant percentage strength?
An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography.The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.
An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.
How many mL of 13x stock solution would you need to make 387 mL of 1x PBS. Report your answer to 1 decimal place.