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Q: Why is it important to limit the quantity of cells used to prepare a smear?
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Q: What are Romanowsky stains? Name four of these.
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- What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handleWhy is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observedThe presence of a capsule around bacterial cells usually indi-cates their increased disease-causing potential and resistance todisinfection. Capsules are generally viewed by:(a) Spore staining(b) Scanning electron microscopy(c) Gram staining(d) Ziehl-Neelsen staining(e) Negative staining
- The benefits of the negative stain include: Mark all that apply: 1. No shrinkage or distortion of cells due to no heat fixing 2. Because the cells don't take up stain they don't get shrunk or distorted 3. One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells 4. Negatively charged dyes are safer to work with than positively charged dyes 5. One can more accurately determine cell size and shape because the cells stand out against the backgroundobserve the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram staining c. Negative staining d Direct staining eAll the aboveBacteria may be Gram + or Gram - due to their difference in staining. This stain is significant to treating bacterial diseases because A. Some people are immune to one type of bacteria but not the other B. The types of antibiotics prescribed will be based in part on which type of bacteria is causing the infection. C. Gram - always cause disease, Gram positives don't D. You can eliminate a large group of bacteria by knowing if infection is caused by either a Gram + or a Gram - which makes it easier to treat. E. B and D are correct
- 4. You partner stained bacterial cells and saw only the background and not the actual cell was stained. Your partner thought this was a mistake. Please explain what type of staining method this is, how it works and why the background and cell is not stained.Crystal violet ( choose all that apply) A. Is not a good differential stain B. Will stain the cytoplasm of the cell walls C. Can be used to stain peptidoglycan on bacteria cell wall D. Can be used as an effective antimicrobialIf there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? No; the clumps will wash away later. Yes; the clumps will hide the individual bacteria. The clumps will not stain. Yes; the clumps of bacterial cells may retain the stains and lead to incorrect data being collected. SubmitRequest Answer
- Which of the following describes the usefulness of a Gram stain. Select all that apply. a. A Gram stain can be used to check for bacteria at the site of a suspected infection or in certain body fluids, such as blood or urine. b. A Gram stain can be used to determine whether penicillin will be effective at treating the patient or whether a different antibiotic should be used. c. A Gram stain can determine whether the bacteria have a thick or thin layer of peptidoglycan in their cell wall.How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: A smear made from liquid media will be thinner than a smear made from solid media A smear made from solid media doesn't have to be fixed but a smear from liquid media does A smear made from liquid media will be more thick than a smear made from solid Water must be added to a smear made from solid media but not liquid media A smear made from liquid media doesn't have to be fixed but a smear from solid media does Water must be added to a smear from liquid media but not from solidWhat is the purpose of a bacterial smear? What is the purpose of a bacterial smear? It spreads the bacteria out into a single layer. It kills the bacteria. It helps the bacteria stick to the slide so they do not wash off during staining. All of the above. None of the above.