What is the purpose of making a serial dilution?

Q: Define base excision pair

A: Base excision repair can be defined as the mechanism in which DNA correction is done by correcting…

Q: How are the Kato Katz Technique results reported?

A: The Kato–Katz technique is a laboratory procedure for preparing human stool samples  parasite egg…

Q: What is the methods used for identifying unknown bacteria?

A: Ability of identifying various microorganisms have many important application.For example,in the…

Q: How is the fragment cut and annealed to the vector?

A: Vector is the organism whose DNA is being used to carry the part of DNA from another organism. In…

Q: How would I know if my PCR product is pure?

A: Polymerase Chain Reaction (PCR) is one of the most significant tools of molecular biology. It was…

Q: Why is pure culture and culture collection important in identifying novel species of bacteria?

A: A pure culture is a population of cells or multicellular organisms growing in the absence of other…

Q: Based on the same two strains of E. coli from the previous question, why do you see growth in the…

A: Ampicillin is a penicillin type of antibiotic substances containing medium So the  strain II can…

Q: What order should the steps be in for this culture method?

A: A conditional mutant is a mutant that exhibits a wild-type phenotype under permissive conditions but…

Q: How can the enrichment culture technique be used medically?

A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…

Q: What is the purpose of the TSI test?

A: A triple sugar iron test or TSI test is used to test a microorganism's ability to ferment sugar and…

Q: Why was it necessary to soak the gel in hydroxide before performing the blot?

A: Gel electrophoresis is a technique which is used to separate the molecules based on the size of the…

Q: What is the application of differential centrifugation?

A: In the laboratories, different kinds of techniques and devices are used to separate components of a…

Q: What are the advantages and disadvantages of multitest systems for bacterial identification?

A: A multitest system is the combination of multiple biochemical tests useful in identification for the…

Q: What is the purpose of the S-9 in the Ames test?

A: The Ames test is a generally utilized technique that utilizes bacteria to test whether a given…

Q: What would a yellow green spot on the microarray mean?

A: The microarray is a technique to determine the expression of thousands of genes at the same time.…

Q: Why do serial dilution? Does serial dilution make it easier to count bacteria on a spread/streak…

A: Dilution is the process of making a solution weaker or less concentrated.

Q: What is the difference between streak plate method and spread plate technique?

A: Answer of the question given below...

Q: What is the procedure of beta method?

A: Beta-lactamase test is used for the detection of enzyme beta-lactamase. This enzyme provides the…

Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…

A: What is motility? Motility is the ability of an organism to move by itself. Eucaryotic cells use…

Q: What is the focus of the Housemann-Rodrik-Velasco growth diagnostic framework?

A: This growth diagnostic framework was give by Hausmann-Rodrik-Velasco.Framework suggests an…

Q: What are the readings that are displayed on the screen of the semi automated analyzer?

A: Before the arrival of automated machines, scientists used to conduct reactions on test tubes or…

Q: What is the purpose of Aseptic technique?

A: The living environment is always surrounded by various microbes, the room temperature paves the…

Q: How can a suspect bacterium be fingerprinted?

A: DNA fingerprinting is a technique for detecting a high number of minisatellites in the genome at the…

Q: When using the blue/ white colony screening method, what do blue colonies represent ?

A: Blue- white screening is used for the identification of recombinant bacteria.

Q: If the five-digit code that is tabulated cannot be found in the EnteroPluri-Test Codebook, what that…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: What is the principle of the gel electrophoresis separation technique?

A: Biological techniques are procedures or strategies for studying biological things. Biological…

Q: What is the proper way of Modified Allen test?

A: Allen test are the tests used to assess the arterial blood supply of the hands. This test is used…

Q: Name the substance used as a medium in gel electrophoresis.

A: Electrophoresis is the process of separation of charged macromolecules under the influence of…

Q: How was Escherichia coli modified to produce a photograph?

A: Bacteria are a group of prokaryotic microscopic single celled organisms. They live in diverse…

Q: What is the Results of the fluctuation test and replica platingexperiments?

A: Mutation is defined as a change or alteration in the nucleotide sequence which in turn alters the…

Q: What is the procedure for methylene blue stain on e.coli?

A: Methylene Blue is a cationic stain, which binds to negatively charged parts of the cells, such as…

Q: How does the agar dilution method differ from streaking toobtain isolated colonies?

A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…

Q: what are two differences between agarose gel electrophoresis and SDS PAGE?

A: Agarose gel electrophoresis and the SDS page technique are used to separate the molecules. The…

Q: What is the use of a “ladder” on a gel electrophoresis?

A: When there is a mixture of nucleic acids and proteins, they can be separated according to their…

Q: Can gel electrophoresis be a quantitative technique? How so?

A: DNA, proteins, RNA are biomolecules which are very crucial in a cell. Many different techniques are…

Q: Why does RNA give a positive result to Biuret's test?

A: Biuret test is a chemical test used for detecting the presence of peptide bonds. In presence of…

Q: How to identify the type of genomic DNA isolation contamination and how to eliminate this…

A: It is chromosomal DNA, in contrast to extra chromosomal DNAs like plasmids. Most organisms have the…

Q: What are the different manufacturing methods of Suppositories dosage form? Explain heat molding…

A: Suppositories are the solid medication  that delivers drug in rectum ,vagina and or urethera. Most…

Q: What could be done to further isolate organisms if multiple types of colonies were found on the…

A: Streaking is the simple process of isolation dilution which is performed by using a sterile tool,…

Q: What is gel electrophoresis?

A: All living systems are made up of chemical molecules, which interacts with each other to perform…

Q: What was the purpose for doing the colony PCR?

A: Colony PCR is a method done to screen the colonies of yeast or bacteria that have grown with the…

Q: If the five-digit code that is tabulated cannot be found in the EnteroPluri-Test Codebook, what…

A: Introduction: EnteroPluri-Test is an identification system that offers complete identification of…

Q: How Gel electrophoresis is used ?

A: DNA profiling is the process of determining an individual's unique DNA pattern. DNA profiling can…

Q: What technique is used to stain bacteria? Who developed this technique?

A: Microorganisms are such small living organisms that are less than 0.1 mm, and can be seen only under…

Q: Why do DNA polymerases fromthermophilic microbes significantly improve the PCRprocedure?

A: Step 1 :Introduction PCR has been used widely in fields such as forensics, genetic testing, and…

Q: What is the purpose of using the streak plate method?

A: Solution-  Introduction -  Streak plate technique is used  for the isolation into a pure culture of…

Q: What was the direction of centrifugation (which fractions correspond to the bottom of the centrifuge…

A: Centrifugal force : centrifugal force is the force that applies on a particle moving in a circular…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.