What is the procedure for methylene blue stain on e.coli?

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing
Problem 2RQ
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What is the procedure for methylene blue stain on e.coli?

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Methylene Blue is a cationic stain, which binds to negatively charged parts of the cells, such as nucleus and RNA in the cytoplasm. It is a common stain used in the identification of bacteria, cellular structure in both plant and animal cells.  

 

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