What is the total size of the mature i.e. fully processed mRNA in nucleotides? How many amino acids would the encoded protein be? Assume that the N- terminal Met encoded by the AUG start codon, is NOT cleaved from the protein?

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter29: Transcription And The Regulation Of Gene Expression
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What is the total size of the mature i.e. fully processed mRNA in nucleotides? How many amino acids would the encoded protein be? Assume that the N- terminal Met encoded by the AUG start codon, is NOT cleaved from the protein?
Part B
A hypothetical mouse gene Minnie (Min) has the following gene structure:
A
5',
3'
B
Transcription start
site
5' X₁
5'UTR
202 105
AUG
312
Transcription termination
62
UAG
280
site
3'UTR
3'
5'
(A) 24 3′
The top diagram (A) shows the gene structure of the Minnie gene and the lower
panel (B) shows the mature mRNA generated from that gene by transcription and
splicing, which is exported to the cytoplasm for translation.
In panel A the size of the exons (black) and introns (grey) are given in basepairs.
The location of the transcription start site and transcription termination site are
indicated by the arrows.
In panel B the 5' untranslated region (5'UTR) of the mRNA is 61 nucleotides
(excluding the modified base X at the 5' end) and the 3' untranslated region
(3′UTR) is 127 nucleotides: This includes a 24 nucleotide poly-A sequence added
post-transcriptionally at the 3' end.
NB: You can assume that the poly-A sequence is added to the 3′ end of the third
exon i.e. as defined by the transcription termination site.
Transcribed Image Text:Part B A hypothetical mouse gene Minnie (Min) has the following gene structure: A 5', 3' B Transcription start site 5' X₁ 5'UTR 202 105 AUG 312 Transcription termination 62 UAG 280 site 3'UTR 3' 5' (A) 24 3′ The top diagram (A) shows the gene structure of the Minnie gene and the lower panel (B) shows the mature mRNA generated from that gene by transcription and splicing, which is exported to the cytoplasm for translation. In panel A the size of the exons (black) and introns (grey) are given in basepairs. The location of the transcription start site and transcription termination site are indicated by the arrows. In panel B the 5' untranslated region (5'UTR) of the mRNA is 61 nucleotides (excluding the modified base X at the 5' end) and the 3' untranslated region (3′UTR) is 127 nucleotides: This includes a 24 nucleotide poly-A sequence added post-transcriptionally at the 3' end. NB: You can assume that the poly-A sequence is added to the 3′ end of the third exon i.e. as defined by the transcription termination site.
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